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. 1987 Aug;55(8):1867–1872. doi: 10.1128/iai.55.8.1867-1872.1987

Characterization of murine monoclonal antibodies that recognize neutralizing epitopes on human C5a.

J W Larrick, J Wang, B M Fendly, D E Chenoweth, S L Kunkel, T Deinhart
PMCID: PMC260616  PMID: 2440808

Abstract

We generated a panel of 10 murine monoclonal antibodies (MAbs) that recognize human complement fragment C5a. These MAbs were characterized for their ability to immunoprecipitate 125I-labeled C5a, bind C5a in solid-phase enzyme immunoassay, and block 125I-labeled C5a binding to polymorphonuclear leukocytes. Four of these MAbs had affinity constants for C5a in the 1 X 10(9) to 3 X 10(9) M-1 range. These MAbs blocked C5a-induced neutrophil polarization and chemiluminescence. They blocked the ability of passively administered C5a to cause neutropenia in rabbits. These anti-C5a neutralizing MAbs may have potential therapeutic use in states of complement activation.

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Selected References

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