Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1987 Sep;55(9):2110–2116. doi: 10.1128/iai.55.9.2110-2116.1987

Stimulation of bone resorption by inflamed nasal mucosa, dermonecrotic toxin-containing conditioned medium from Pasteurella multocida, and purified dermonecrotic toxin from P. multocida.

T G Kimman, C W Löwik, L J van de Wee-Pals, C W Thesingh, P Defize, E M Kamp, O L Bijvoet
PMCID: PMC260664  PMID: 3623695

Abstract

The effects of inflamed nasal mucosa from pigs with atrophic rhinitis (AR), cell extract from Bordetella bronchiseptica, conditioned medium from Pasteurella multocida, and purified dermonecrotic toxin (DNT) from P. multocida on mouse fetal long bones in organ culture were studied. Inflamed nasal "AR mucosa" stimulated the release of 45Ca from prelabeled cultures, while histologically the formation of calcified matrix was impaired as well. B. bronchiseptica cell extract only transiently increased 45Ca release, but also impaired the formation of matrix. 45Ca release was also stimulated by DNT-containing conditioned medium from P. multocida and by purified DNT. The effect of DNT was biphasic: low doses (1 to 25 ng/ml) slightly stimulated bone resorption, higher doses were inhibitory. The stimulatory action of DNT on 45Ca release was accompanied by an increase in numbers of preosteoclasts and osteoclasts. The significance of these findings for the pathogenesis of AR is discussed.

Full text

PDF
2110

Images in this article

2112Image
on p.2112
2114Image
on p.2114

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertolini D. R., Nedwin G. E., Bringman T. S., Smith D. D., Mundy G. R. Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986 Feb 6;319(6053):516–518. doi: 10.1038/319516a0. [DOI] [PubMed] [Google Scholar]
  2. Dewhirst F. E. N-acetyl muramyl dipeptide stimulation of bone resorption in tissue culture. Infect Immun. 1982 Jan;35(1):133–137. doi: 10.1128/iai.35.1.133-137.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dewhirst F. E., Stashenko P. P., Mole J. E., Tsurumachi T. Purification and partial sequence of human osteoclast-activating factor: identity with interleukin 1 beta. J Immunol. 1985 Oct;135(4):2562–2568. [PubMed] [Google Scholar]
  4. Dominick M. A., Rimler R. B. Turbinate atrophy in gnotobiotic pigs intranasally inoculated with protein toxin isolated from type D Pasteurella multocida. Am J Vet Res. 1986 Jul;47(7):1532–1536. [PubMed] [Google Scholar]
  5. Elling F., Pedersen K. B. The pathogenesis of persistent turbinate atrophy induced by toxigenic Pasteurella multocida in pigs. Vet Pathol. 1985 Sep;22(5):469–474. doi: 10.1177/030098588502200506. [DOI] [PubMed] [Google Scholar]
  6. Fetter A. W., Switzer W. P., Capen C. C. Electron microscopic evaluation of bone cells in pigs with experimentally induced Bordetella rhinitis (turbinate osteoporosis). Am J Vet Res. 1975 Jan;36(1):15–22. [PubMed] [Google Scholar]
  7. Goodson J. M., Dewhirst F. E., Brunetti A. Prostaglandin E2 levels and human periodontal disease. Prostaglandins. 1974 Apr 10;6(1):81–85. doi: 10.1016/s0090-6980(74)80043-2. [DOI] [PubMed] [Google Scholar]
  8. Hausmann E., Lüderitz O., Knox K., Weinfeld N. Structural requirements for bone resorption by endotoxin and lipoteichoic acid. J Dent Res. 1975 Jun;54(SPEC):B94–B99. doi: 10.1177/00220345750540023401. [DOI] [PubMed] [Google Scholar]
  9. Hausmann E., Raisz L. G., Miller W. A. Endotoxin: stimulation of bone resorption in tissue culture. Science. 1970 May 15;168(3933):862–864. doi: 10.1126/science.168.3933.862. [DOI] [PubMed] [Google Scholar]
  10. Heath J. K., Saklatvala J., Meikle M. C., Atkinson S. J., Reynolds J. J. Pig interleukin 1 (catabolin) is a potent stimulator of bone resorption in vitro. Calcif Tissue Int. 1985 Jan;37(1):95–97. doi: 10.1007/BF02557686. [DOI] [PubMed] [Google Scholar]
  11. Holtrop M. E., Raisz L. G. Comparison of the effects of 1,25-dihydroxycholecalciferol, prostaglandin E2, and osteoclast-activating factor with parathyroid hormone on the ultrastructure of osteoclasts in cultured long bones of fetal rats. Calcif Tissue Int. 1979;29(3):201–205. doi: 10.1007/BF02408081. [DOI] [PubMed] [Google Scholar]
  12. Hopps R. M., Nuki K., Raisz L. G. Demonstration and preliminary characterization of bone resorbing activity in freeze-dried gingiva of dogs. Calcif Tissue Int. 1980;31(3):239–245. doi: 10.1007/BF02407187. [DOI] [PubMed] [Google Scholar]
  13. Horton J. E., Raisz L. G., Simmons H. A., Oppenheim J. J., Mergenhagen S. E. Bone resorbing activity in supernatant fluid from cultured human peripheral blood leukocytes. Science. 1972 Sep 1;177(4051):793–795. doi: 10.1126/science.177.4051.793. [DOI] [PubMed] [Google Scholar]
  14. Kamp E. M., Van der Heijden P. J., Tetenburg B. J. Purification of a heat labile dermonecrotic toxin from culture fluid of Pasteurella multocida. Vet Microbiol. 1987 Mar;13(3):235–248. doi: 10.1016/0378-1135(87)90086-1. [DOI] [PubMed] [Google Scholar]
  15. Lensgraf E. J., Greenblatt J. J., Bawden J. W. Effect of group A streptococcal peptidoglycan and group A streptococcal cell wall on bone in tissue culture. Arch Oral Biol. 1979;24(7):495–498. doi: 10.1016/0003-9969(79)90126-2. [DOI] [PubMed] [Google Scholar]
  16. Nakai T., Sawata A., Kume K. Intracellular locations of dermonecrotic toxins in Pasteurella multocida and in Bordetella bronchiseptica. Am J Vet Res. 1985 Apr;46(4):870–874. [PubMed] [Google Scholar]
  17. Nakai T., Sawata A., Tsuji M., Samejima Y., Kume K. Purification of dermonecrotic toxin from a sonic extract of Pasteurella multocida SP-72 serotype D. Infect Immun. 1984 Nov;46(2):429–434. doi: 10.1128/iai.46.2.429-434.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Novotny P., Chubb A. P., Cownley K., Montaraz J. A. Adenylate cyclase activity of a 68,000-molecular-weight protein isolated from the outer membrane of Bordetella bronchiseptica. Infect Immun. 1985 Oct;50(1):199–206. doi: 10.1128/iai.50.1.199-206.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pedersen K. B., Elling F. The pathogenesis of atrophic rhinitis in pigs induced by toxigenic Pasteurella multocida. J Comp Pathol. 1984 Apr;94(2):203–214. doi: 10.1016/0021-9975(84)90041-0. [DOI] [PubMed] [Google Scholar]
  20. Rutter J. M., Mackenzie A. Pathogenesis of atrophic rhinitis in pigs: a new perspective. Vet Rec. 1984 Jan 28;114(4):89–90. doi: 10.1136/vr.114.4.89. [DOI] [PubMed] [Google Scholar]
  21. Rutter J. M., Taylor R. J., Crighton W. G., Robertson I. B., Benson J. A. Epidemiological study of Pasteurella multocida and Bordetella bronchiseptica in atrophic rhinitis. Vet Rec. 1984 Dec 15;115(24):615–619. doi: 10.1136/vr.115.24.615. [DOI] [PubMed] [Google Scholar]
  22. Rutter J. M. Virulence of Pasteurella multocida in atrophic rhinitis of gnotobiotic pigs infected with Bordetella bronchiseptica. Res Vet Sci. 1983 May;34(3):287–295. [PubMed] [Google Scholar]
  23. Scheven B. A., Kawilarang-De Haas E. W., Wassenaar A. M., Nijweide P. J. Differentiation kinetics of osteoclasts in the periosteum of embryonic bones in vivo and in vitro. Anat Rec. 1986 Apr;214(4):418–423. doi: 10.1002/ar.1092140413. [DOI] [PubMed] [Google Scholar]
  24. Schöss P., Thiel C. P., Schimmelpfennig H. Rhinitis atrophicans des Schweines: Untersuchungen über das Vorkommen toxinbildender Stämme von Pasteurella multocida und Bordetella bronchiseptica. Dtsch Tierarztl Wochenschr. 1985 Sep 6;92(9):316–319. [PubMed] [Google Scholar]
  25. Silveira D., Edington N., Smith I. M. Ultrastructural changes in the nasal turbinate bones of pigs in early infection with Bordetella bronchiseptica. Res Vet Sci. 1982 Jul;33(1):37–42. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES