Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1987 Sep;55(9):2314–2316. doi: 10.1128/iai.55.9.2314-2316.1987

Transposon mutagenesis of group B streptococcus beta-hemolysin biosynthesis.

J N Weiser, C E Rubens
PMCID: PMC260700  PMID: 3040595

Abstract

Beta-hemolysin production by group B streptococci (GBS) is speculated to be a major virulence factor of the organism. A virulent, beta-hemolytic group B streptococcus strain was mutagenized with the self-conjugative transposon Tn916 to derive isogenic strains with mutations only in the gene(s) responsible for beta-hemolysin biosynthesis. There was no significant difference between the virulence of the parent strain and that of the mutant strains in a neonatal rat sepsis model.

Full text

PDF
2314

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gaillard J. L., Berche P., Sansonetti P. Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect Immun. 1986 Apr;52(1):50–55. doi: 10.1128/iai.52.1.50-55.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gawron-Burke C., Clewell D. B. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria. J Bacteriol. 1984 Jul;159(1):214–221. doi: 10.1128/jb.159.1.214-221.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hull R. A., Gill R. E., Hsu P., Minshew B. H., Falkow S. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun. 1981 Sep;33(3):933–938. doi: 10.1128/iai.33.3.933-938.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ike Y., Hashimoto H., Clewell D. B. Hemolysin of Streptococcus faecalis subspecies zymogenes contributes to virulence in mice. Infect Immun. 1984 Aug;45(2):528–530. doi: 10.1128/iai.45.2.528-530.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Marchlewicz B. A., Duncan J. L. Properties of a hemolysin produced by group B streptococci. Infect Immun. 1980 Dec;30(3):805–813. doi: 10.1128/iai.30.3.805-813.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nida K., Cleary P. P. Insertional inactivation of streptolysin S expression in Streptococcus pyogenes. J Bacteriol. 1983 Sep;155(3):1156–1161. doi: 10.1128/jb.155.3.1156-1161.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  8. Roe M. H., Todd J. K., Favara B. E. Nonhemolytic group B streptococcal infections. J Pediatr. 1976 Jul;89(1):75–77. doi: 10.1016/s0022-3476(76)80931-6. [DOI] [PubMed] [Google Scholar]
  9. Zeligs B. J., Armstrong C. D., Walser J. B., Bellanti J. A. Age-dependent susceptibility of neonatal rats to group B streptococcal type III infection: correlation of severity of infection and response of myeloid pools. Infect Immun. 1982 Jul;37(1):255–263. doi: 10.1128/iai.37.1.255-263.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES