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. 2008 Nov 25;5:104. doi: 10.1186/1742-4690-5-104

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Copyright © 2008 Kim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoblot analysis of CAgag in culture media from ICR-A, R1A and P8A cell lines. (A) Western blot analysis for expression of CAgag (40 μl of culture media) using anti-CAgag antibody. Zpl 2-1 culture media: a negative control; SAMP8 brain (12-month-old) and P8A9 cell lysate (P8A9-CL) (50 μg): positive controls. (B) Densitometry of CAgag in cell culture media. CAgag protein levels in P8A cell culture media were significantly higher than in R1A cell culture media. *statistically significant difference (p < 0.01).