Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1989 Oct;57(10):3234–3236. doi: 10.1128/iai.57.10.3234-3236.1989

Virulence, persistence, and immunogenicity of Yersinia enterocolitica O:8 aroA mutants.

F Bowe 1, P O'Gaora 1, D Maskell 1, M Cafferkey 1, G Dougan 1
PMCID: PMC260796  PMID: 2777382

Abstract

The virulent Yersinia enterocolitica strain 8081 killed BALB/c mice within 5 days of oral infection with a 50% lethal dose of log10 7.1, whereas an aroA mutant of 8081, YAM.1, and the plasmidless variant 8081c failed to kill mice. Unlike 8081, YAM.1 and 8081c did not persist or grow in the Peyer's patches, mesenteric lymph nodes, livers, or spleens of mice. Mice immunized orally with single doses of live YAM.1 were poorly protected against a lethal 8081 challenge, whereas mice immunized with three doses of YAM.1 were moderately well protected.

Full text

PDF
3234

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahvonen P., Sievers K., Aho K. Arthritis associated with Yersinia enterocolitica infection. Acta Rheumatol Scand. 1969;15(3):232–253. doi: 10.3109/rhe1.1969.15.issue-1-4.32. [DOI] [PubMed] [Google Scholar]
  2. Black R. E., Jackson R. J., Tsai T., Medvesky M., Shayegani M., Feeley J. C., MacLeod K. I., Wakelee A. M. Epidemic Yersinia enterocolitica infection due to contaminated chocolate milk. N Engl J Med. 1978 Jan 12;298(2):76–79. doi: 10.1056/NEJM197801122980204. [DOI] [PubMed] [Google Scholar]
  3. Bradford W. D., Noce P. S., Gutman L. T. Pathologic features of enteric infection with Yersinia enterocolitica. Arch Pathol. 1974 Jul;98(1):17–22. [PubMed] [Google Scholar]
  4. Carniel E., Mazigh D., Mollaret H. H. Expression of iron-regulated proteins in Yersinia species and their relation to virulence. Infect Immun. 1987 Jan;55(1):277–280. doi: 10.1128/iai.55.1.277-280.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carter P. B. Pathogenecity of Yersinia enterocolitica for mice. Infect Immun. 1975 Jan;11(1):164–170. doi: 10.1128/iai.11.1.164-170.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chiesa C., Pacifico L., Cianfrano V., Midulla M. Italian experience with yersiniosis (1978-1985). Contrib Microbiol Immunol. 1987;9:76–88. [PubMed] [Google Scholar]
  7. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cornelis G., Laroche Y., Balligand G., Sory M. P., Wauters G. Yersinia enterocolitica, a primary model for bacterial invasiveness. Rev Infect Dis. 1987 Jan-Feb;9(1):64–87. doi: 10.1093/clinids/9.1.64. [DOI] [PubMed] [Google Scholar]
  9. Gutman L. T., Ottesen E. A., Quan T. J., Noce P. S., Katz S. L. An inter-familial outbreak of Yersinia enterocolitica enteritis. N Engl J Med. 1973 Jun 28;288(26):1372–1377. doi: 10.1056/NEJM197306282882604. [DOI] [PubMed] [Google Scholar]
  10. Hoiseth S. K., Stocker B. A. Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines. Nature. 1981 May 21;291(5812):238–239. doi: 10.1038/291238a0. [DOI] [PubMed] [Google Scholar]
  11. Leirisalo-Repo M. Yersinia arthritis. Acute clinical picture and long-term prognosis. Contrib Microbiol Immunol. 1987;9:145–154. [PubMed] [Google Scholar]
  12. Lian C. J., Hwang W. S., Kelly J. K., Pai C. H. Penetration of the intestinal mucosa by Yersinia enterocolitica lacking the virulence plasmid. Contrib Microbiol Immunol. 1987;9:239–242. [PubMed] [Google Scholar]
  13. Maskell D. J., Sweeney K. J., O'Callaghan D., Hormaeche C. E., Liew F. Y., Dougan G. Salmonella typhimurium aroA mutants as carriers of the Escherichia coli heat-labile enterotoxin B subunit to the murine secretory and systemic immune systems. Microb Pathog. 1987 Mar;2(3):211–221. doi: 10.1016/0882-4010(87)90022-2. [DOI] [PubMed] [Google Scholar]
  14. Mollaret H. H. L'infection humaine a "Yersinia enterocolitica". Pathol Biol. 1966 Oct;14(19):981–990. [PubMed] [Google Scholar]
  15. O'Callaghan D., Maskell D., Liew F. Y., Easmon C. S., Dougan G. Characterization of aromatic- and purine-dependent Salmonella typhimurium: attention, persistence, and ability to induce protective immunity in BALB/c mice. Infect Immun. 1988 Feb;56(2):419–423. doi: 10.1128/iai.56.2.419-423.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schiemann D. A., Devenish J. A. Virulence of Yersinia enterocolitica determined by lethality in Mongolian gerbils and by the Serény test. Infect Immun. 1980 Aug;29(2):500–506. doi: 10.1128/iai.29.2.500-506.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simonet M., Berche P., Mazigh D., Veron M. Protection against Yersinia infection induced by non-virulence-plasmid-encoded antigens. J Med Microbiol. 1985 Oct;20(2):225–231. doi: 10.1099/00222615-20-2-225. [DOI] [PubMed] [Google Scholar]
  19. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES