Abstract
Monoclonal antibodies (MAbs) of two different specificities were produced by immunizing mice with the semipurified M antigen of histoplasmin. One type, from clone CB4, was an immunoglobulin M that precipitated a polysaccharide present in histoplasmin and also formed immunoprecipitates with a cross-reactive polysaccharide present in extracts of Blastomyces dermatitidis and Coccidioides immitis. The second type of MAb, from clone EC2, was an immunoglobulin G that reacted in the enzyme-linked immunoelectrotransfer blot (EITB) assay with a doublet of proteins with an apparent molecular size of 70 to 75 kilodaltons. This molecule is proposed as the authentic M protein antigen that is recognized by M antibodies in sera from mice and rabbits immunized with Histoplasma capsulatum and from persons with histoplasmosis. The M factor also occurs in an abundant disulfide-bridged dimer which has a molecular size of 150 kilodaltons and is nonimmunoreactive under the conditions of sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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- Azuma I., Kanetsuna F., Tanaka Y., Yamamura Y., Carbonell L. M. Chemical and immunological properties of galactomannans obtained from Histoplasma duboisii, Histoplasma capsulatum, Paracoccidioides brasiliensis and Blasomyces dermatitidis. Mycopathol Mycol Appl. 1974 Oct 15;54(1):111–125. doi: 10.1007/BF02055979. [DOI] [PubMed] [Google Scholar]
- Biguet J., Tran-Van-Ky P., Andrieu S., Vaucelle T. Premières caractérisations d'activités enzymatiques sur les immunoélectrophorégrammes des extraits antigéniques de Histoplasma capsulatum. Conséquences diagnostiques pratiques. Ann Soc Belges Med Trop Parasitol Mycol. 1967;47(6):425–433. [PubMed] [Google Scholar]
- Bradley G., Pine L., Reeves M. W., Moss C. W. Purification, composition, and serological characterization of histoplasmin-H and M antigens. Infect Immun. 1974 May;9(5):870–880. doi: 10.1128/iai.9.5.870-880.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRABAR P., WILLIAMS C. A., Jr Méthode immuno-électrophorétique d'analyse de mélanges de substances antigéniques. Biochim Biophys Acta. 1955 May;17(1):67–74. doi: 10.1016/0006-3002(55)90320-6. [DOI] [PubMed] [Google Scholar]
- HEINER D. C. Diagnosis of histoplasmosis using precipitin reactions in agargel. Pediatrics. 1958 Oct;22(4 Pt 1):616–627. [PubMed] [Google Scholar]
- Pine L., Gross H., Malcolm G. B., George J. R., Gray S. B., Moss C. W. Procedures for the production and separation of H and M antigens in histoplasmin: chemical and serological properties of the isolated products. Mycopathologia. 1977 Oct 28;61(3):131–141. doi: 10.1007/BF00468007. [DOI] [PubMed] [Google Scholar]
- Pine L., Malcolm G. B., Gross H., Gray S. B. Evaluation of purified H and M antigens of histoplasmin as reagents in the complement fixation test. Sabouraudia. 1978 Dec;16(4):257–269. doi: 10.1080/00362177885380361. [DOI] [PubMed] [Google Scholar]
- Tsang V. C., Peralta J. M., Simons A. R. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 1983;92:377–391. doi: 10.1016/0076-6879(83)92032-3. [DOI] [PubMed] [Google Scholar]
- Wheat L. J., Kohler R. B., Tewari R. P. Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N Engl J Med. 1986 Jan 9;314(2):83–88. doi: 10.1056/NEJM198601093140205. [DOI] [PubMed] [Google Scholar]