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. 1986 Aug;53(2):294–297. doi: 10.1128/iai.53.2.294-297.1986

Endotoxin-inactivating activity in normal and pathological human blood samples.

T Obayashi, H Tamura, S Tanaka, M Ohki, S Takahashi, T Kawai
PMCID: PMC260873  PMID: 3733219

Abstract

The endotoxin-specific chromogenic test revealed that plasma endotoxin-inactivating activity was markedly diminished by endotoxemia, but not by fungemia or by dialysis with cellulose membranes, suggesting that fungal polysaccharides and other nonendotoxic, Limulus-reactive materials do not consume endotoxin-inactivating factors in the blood. There was a close negative correlation between plasma endotoxin concentration and endotoxin-inactivating activity. The specificity of the test was improved by fractionating amebocyte lysate and using only the factors that constitute the endotoxin-sensitive coagulation pathway of the horseshoe crab. This test was able to differentiate endotoxemia from fungemia and from contamination with other nonendotoxic, Limulus-reactive materials.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Johnson K. J., Ward P. A., Goralnick S., Osborn M. J. Isolation from human serum of an inactivator of bacterial lipopolysaccharide. Am J Pathol. 1977 Sep;88(3):559–574. [PMC free article] [PubMed] [Google Scholar]
  2. Johnson K. J., Ward P. A. The requirement for serum complement in the detoxification of bacterial endotoxin. J Immunol. 1972 Mar;108(3):611–616. [PubMed] [Google Scholar]
  3. KEENE W. R., LANDY M., SHEAR M. J. Inactivation of endotoxin by a humoral component. VII. Enzymatic degradation of endotoxin by blood plasma. J Clin Invest. 1961 Feb;40:302–310. doi: 10.1172/JCI104257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mathison J. C., Ulevitch R. J. The clearance, tissue distribution, and cellular localization of intravenously injected lipopolysaccharide in rabbits. J Immunol. 1979 Nov;123(5):2133–2143. [PubMed] [Google Scholar]
  5. Nakamura T., Morita T., Iwanaga S. Lipopolysaccharide-sensitive serine-protease zymogen (factor C) found in Limulus hemocytes. Isolation and characterization. Eur J Biochem. 1986 Feb 3;154(3):511–521. doi: 10.1111/j.1432-1033.1986.tb09427.x. [DOI] [PubMed] [Google Scholar]
  6. Obayashi T. Addition of perchloric acid to blood samples for colorimetric limulus test using chromogenic substrate: comparison with conventional procedures and clinical applications. J Lab Clin Med. 1984 Sep;104(3):321–330. [PubMed] [Google Scholar]
  7. Obayashi T., Tamura H., Tanaka S., Ohki M., Takahashi S., Arai M., Masuda M., Kawai T. A new chromogenic endotoxin-specific assay using recombined limulus coagulation enzymes and its clinical applications. Clin Chim Acta. 1985 Jun 30;149(1):55–65. doi: 10.1016/0009-8981(85)90273-6. [DOI] [PubMed] [Google Scholar]
  8. Pearson F. C., Bohon J., Lee W., Bruszer G., Sagona M., Dawe R., Jakubowski G., Morrison D., Dinarello C. Comparison of chemical analyses of hollow-fiber dialyzer extracts. Artif Organs. 1984 Aug;8(3):291–298. doi: 10.1111/j.1525-1594.1984.tb04293.x. [DOI] [PubMed] [Google Scholar]
  9. Skarnes R. C. In vivo interaction of endotoxin with a plasma lipoprotein having esterase activity. J Bacteriol. 1968 Jun;95(6):2031–2034. doi: 10.1128/jb.95.6.2031-2034.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ulevitch R. J., Johnston A. R., Weinstein D. B. New function for high density lipoproteins. Isolation and characterization of a bacterial lipopolysaccharide-high density lipoprotein complex formed in rabbit plasma. J Clin Invest. 1981 Mar;67(3):827–837. doi: 10.1172/JCI110100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Yoshioka M., Konno S. Characteristics of Endotoxin-Altering Fractions Derived from Normal Serum III. Isolation and Properties of Horse Serum alpha(2)-Macroglobulin. Infect Immun. 1970 May;1(5):431–439. doi: 10.1128/iai.1.5.431-439.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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