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. 1986 Jun;52(3):682–687. doi: 10.1128/iai.52.3.682-687.1986

Local active gingival immunization by a 3,800-molecular-weight streptococcal antigen in protection against dental caries.

T Lehner, A Mehlert, J Caldwell
PMCID: PMC260911  PMID: 3710580

Abstract

Local gingival immunization was attempted in an effort to confine the immune response to the oral cavity and bypass the systemic immune response. A low-molecular-weight (3.8K) streptococcal antigen (SA) I/II was applied 10 times over a period of 1 year to the gingival crevices of rhesus monkeys. The antigen was maintained in situ by means of silicone rubber appliances. Serial examinations over a period of 1 year showed that topical gingival immunization with the 3.8K SA results in a significantly lower incidence of dental caries and colonization of Streptococcus mutans compared with that of the sham-immunized controls. This was associated with an increase in gingival crevicular immunoglobulin G and salivary immunoglobulin A anti-SA I/II antibodies, whereas no change occurred in serum antibodies to SA I/II. The immune mechanism which prevents the colonization of S. mutans and the development of caries may involve antibodies that prevent the adherence of S. mutans to the teeth and facilitate phagocytosis and killing by the local neutrophils. This novel route of local immunization is noninvasive, does not cause side effects, and bypasses systemic immunization.

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Selected References

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  1. Bowen W. H., Cohen B., Cole M. F., Colman G. Immunization against dental caries. Br Dent J. 1975 Jul 15;139(2):45–58. [PubMed] [Google Scholar]
  2. Caldwell J., Challacombe S. J., Lehner T. A sequential bacteriological and serological investigation of Rhesus monkeys immunised against dental caries with Streptococcus mutans. J Med Microbiol. 1977 May;10(2):213–224. doi: 10.1099/00222615-10-2-213. [DOI] [PubMed] [Google Scholar]
  3. Challacombe S. J., Lehner T. Salivary antibody responses in rhesus monkeys immunized with Streptococcus mutans by the oral, submucosal or subcutaneous routes. Arch Oral Biol. 1979;24(12):917–925. doi: 10.1016/0003-9969(79)90217-6. [DOI] [PubMed] [Google Scholar]
  4. Evans R. T., Emmings F. G., Genco R. J. Prevention of Streptococcus mutans infection of tooth surfaces by salivary antibody in Irus monkeys (Macaca fascicularis). Infect Immun. 1975 Aug;12(2):293–302. doi: 10.1128/iai.12.2.293-302.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gahnberg L., Krasse B. Salivary immunoglobulin A antibodies and recovery from challenge of Streptococcus mutans after oral administration of Streptococcus mutans vaccine in humans. Infect Immun. 1983 Feb;39(2):514–519. doi: 10.1128/iai.39.2.514-519.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giasuddin A. S., Lehner T., Evans R. W. Identification, purification and characterization of a streptococcal protein antigen with a molecular weight of 3800. Immunology. 1983 Dec;50(4):651–658. [PMC free article] [PubMed] [Google Scholar]
  7. Krasse B., Jordan H. V. Effect of orally applied vaccines on oral colonization by Streptococcus mutans in rodents. Arch Oral Biol. 1977;22(8-9):479–484. doi: 10.1016/0003-9969(77)90041-3. [DOI] [PubMed] [Google Scholar]
  8. Lehner T., Caldwell J., Avery J. Sequential development of helper and suppressor functions, antibody titers and functional avidities to a streptococcal antigen in rhesus monkeys. Eur J Immunol. 1984 Sep;14(9):814–819. doi: 10.1002/eji.1830140909. [DOI] [PubMed] [Google Scholar]
  9. Lehner T., Caldwell J., Giasuddin A. S. Comparative immunogenicity and protective effect against dental caries of a low (3800) and a high (185,000) molecular weight protein in rhesus monkeys (Macaca mulatta). Arch Oral Biol. 1985;30(3):207–212. doi: 10.1016/0003-9969(85)90034-2. [DOI] [PubMed] [Google Scholar]
  10. Lehner T., Caldwell J., Smith R. Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries. Infect Immun. 1985 Dec;50(3):796–799. doi: 10.1128/iai.50.3.796-799.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lehner T., Challacombe S. J., Caldwell J. An experimental model for immunological studies of dental caries in the rhesus monkey. Arch Oral Biol. 1975 May-Jun;20(5-6):299–304. doi: 10.1016/0003-9969(75)90018-7. [DOI] [PubMed] [Google Scholar]
  12. Lehner T., Challacombe S. J., Caldwell J. Immunological and bacteriological basis for vaccination against dental caries in rhesus monkeys. Nature. 1975 Apr 10;254(5500):517–520. doi: 10.1038/254517a0. [DOI] [PubMed] [Google Scholar]
  13. Lehner T., Challacombe S. J., Caldwell J. Oral immunization with Streptococcus mutants in rhesus monkeys and the development of immune response and dental caries. Immunology. 1980 Dec;41(4):857–864. [PMC free article] [PubMed] [Google Scholar]
  14. Lehner T., Challacombe S. J., Wilton J. M., Caldwell J. Cellular and humoral immune responses in vaccination against dental caries in monkeys. Nature. 1976 Nov 4;264(5581):69–72. doi: 10.1038/264069a0. [DOI] [PubMed] [Google Scholar]
  15. Lehner T., Mehlert A., Avery J., Jones T., Caldwell J. The helper and suppressor functions of primate T cells elicited by a 185K streptococcal antigen, as compared with the helper function elicited by a 4K streptococcal antigen. J Immunol. 1985 Aug;135(2):1437–1442. [PubMed] [Google Scholar]
  16. Lehner T., Russell M. W., Caldwell J., Smith R. Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infect Immun. 1981 Nov;34(2):407–415. doi: 10.1128/iai.34.2.407-415.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lehner T., Russell M. W., Challacombe S. J., Scully C. M., Hawkes J. E. Passive immunisation with serum and immunoglobulins against dental caries in rhesus monkeys. Lancet. 1978 Apr 1;1(8066):693–695. doi: 10.1016/s0140-6736(78)90803-6. [DOI] [PubMed] [Google Scholar]
  18. McBride B. C., Song M., Krasse B., Olsson J. Biochemical and immunological differences between hydrophobic and hydrophilic strains of Streptococcus mutans. Infect Immun. 1984 Apr;44(1):68–75. doi: 10.1128/iai.44.1.68-75.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mestecky J., McGhee J. R., Arnold R. R., Michalek S. M., Prince S. J., Babb J. L. Selective induction of an immune response in human external secretions by ingestion of bacterial antigen. J Clin Invest. 1978 Mar;61(3):731–737. doi: 10.1172/JCI108986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Michalek S. M., McGhee J. R., Mestecky J., Arnold R. R., Bozzo L. Ingestion of Streptococcus mutans induces secretory immunoglobulin A and caries immunity. Science. 1976 Jun 18;192(4245):1238–1240. doi: 10.1126/science.1273589. [DOI] [PubMed] [Google Scholar]
  21. Moro I., Russell M. W. Ultrastructural localization of protein antigens I/II and III in Streptococcus mutans. Infect Immun. 1983 Jul;41(1):410–413. doi: 10.1128/iai.41.1.410-413.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Page R. C., Schroeder H. E. Pathogenesis of inflammatory periodontal disease. A summary of current work. Lab Invest. 1976 Mar;34(3):235–249. [PubMed] [Google Scholar]
  23. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infect Immun. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Russell M. W., Lehner T. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Arch Oral Biol. 1978;23(1):7–15. doi: 10.1016/0003-9969(78)90047-x. [DOI] [PubMed] [Google Scholar]
  25. Schiött C. R., Löe H. The origin and variation in number of leukocytes in the human saliva. J Periodontal Res. 1970;5(1):36–41. doi: 10.1111/j.1600-0765.1970.tb01835.x. [DOI] [PubMed] [Google Scholar]
  26. Scully C. M., Lehner T. Opsonization, phagocytosis and killing of Streptococcus mutans by polymorphonuclear leukocytes, in relation to dental caries in the rhesus monkey (Macaca mulatta). Arch Oral Biol. 1979;24(4):307–312. doi: 10.1016/0003-9969(79)90093-1. [DOI] [PubMed] [Google Scholar]
  27. Skapski H., Lehner T. A crevicular washing method for investigating immune components of crevicular fluid in man. J Periodontal Res. 1976 Feb;11(1):19–24. doi: 10.1111/j.1600-0765.1976.tb00046.x. [DOI] [PubMed] [Google Scholar]
  28. Smith R., Lehner T. A radioimmunoassay for serum and gingival crevicular fluid antibodies to a purified protein of Streptococcus mutans. Clin Exp Immunol. 1981 Feb;43(2):417–424. [PMC free article] [PubMed] [Google Scholar]
  29. Smith R., Lehner T., Beverley P. C. Characterization of monoclonal antibodies to Streptococcus mutans antigenic determinants I/II, I, II, and III and their serotype specificities. Infect Immun. 1984 Oct;46(1):168–175. doi: 10.1128/iai.46.1.168-175.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Talbman M. A., Smith D. J. Effects of local immunization with Streptococcus mutans on induction of salivary immunoglobulin A antibody and experimental dental caries in rats. Infect Immun. 1974 Jun;9(6):1079–1091. doi: 10.1128/iai.9.6.1079-1091.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tolo K., Brandtzaeg P., Jonsen J. Mucosal penetration of antigen in the presence or absence of serum-derived antibody. Immunology. 1977 Nov;33(5):733–743. [PMC free article] [PubMed] [Google Scholar]
  32. Walker J. Antibody responses of monkeys to oral and local immunization with Streptococcus mutans. Infect Immun. 1981 Jan;31(1):61–70. doi: 10.1128/iai.31.1.61-70.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wilton J. M., Renggli H. H., Lehner T. The isolation and identification of mononuclear cells from the gingival crevice in man. J Periodontal Res. 1976 Sep;11(5):262–268. doi: 10.1111/j.1600-0765.1976.tb00080.x. [DOI] [PubMed] [Google Scholar]
  34. Zanders E. D., Lehner T. Separation and characterization of a protein antigen from cells of Streptococcus mutans. J Gen Microbiol. 1981 Feb;122(2):217–225. doi: 10.1099/00221287-122-2-217. [DOI] [PubMed] [Google Scholar]
  35. de Stoppelaar J. D., van Houte J., de Moor C. E. The presence of dextran-forming bacteria, resembling Streptococcus bovis and Streptococcus anguis, in human dental plaque. Arch Oral Biol. 1967 Oct;12(10):1199–1202. doi: 10.1016/0003-9969(67)90069-6. [DOI] [PubMed] [Google Scholar]

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