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Fluorescence intensity decays of WALP23 in the presence of varying concentrations of DHE in bilayers of DMPC at pH 7.0 and 30 °C. The bilayers contained either no cholesterol (A) or equimolar amounts of cholesterol (B). Global analysis of the data according to the model for a random distribution of DHE (eqs 2 and 3) resulted in the fitting curves shown yielding global χG2 values of 1.43 for panel A and 1.35 for panel B.
