Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 May;52(2):390–396. doi: 10.1128/iai.52.2.390-396.1986

Comparison of in vivo degradation of 125I-labeled peptidoglycan-polysaccharide fragments from group A and group D streptococci.

S A Stimpson, R E Esser, W J Cromartie, J H Schwab
PMCID: PMC261011  PMID: 3516872

Abstract

The in vivo degradation and persistence of 125I-labeled peptidoglycan-polysaccharide (PG-PS) fragments from the cell walls of group A and D streptococci were compared by group after intraperitoneal injection into rats. The quantity of PG-PS in the livers and spleens of group D PG-PS-injected rats was less than the quantity in rats injected with group A PG-PS throughout the course of the experiment. Gel filtration analyses of liver and spleen homogenates indicated that group A PG-PS was relatively resistant to degradation, whereas group D PG-PS was extensively degraded to yield a heterogeneous mixture of fragments of lower molecular weight. There was no significant difference in the content of group A PG-PS versus that of group D in joints or blood samples. Analysis of fragment sizes in these tissues also indicated more extensive degradation of group D PG-PS. However, the majority of group A PG-PS in blood samples and joints was a lower molecular weight than that found in the livers or spleens. We conclude that group A PG-PS undergoes a significant but low level of degradation and that group D PG-PS is much less persistent and more extensively degraded than group A PG-PS is in vivo. These differences in PG-PS catabolism may account, in part, for the capacity of group A PG-PS to induce chronic, recurrent arthritis of longer duration than that induced by group D PG-PS.

Full text

PDF
390

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chetty C., Klapper D. G., Schwab J. H. Soluble peptidoglycan-polysaccharide fragments of the bacterial cell wall induce acute inflammation. Infect Immun. 1982 Dec;38(3):1010–1019. doi: 10.1128/iai.38.3.1010-1019.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark R. L., Cuttino J. T., Jr, Anderle S. K., Cromartie W. J., Schwab J. H. Radiologic analysis of arthritis in rats after systemic injection of streptococcal cell walls. Arthritis Rheum. 1979 Jan;22(1):25–35. doi: 10.1002/art.1780220105. [DOI] [PubMed] [Google Scholar]
  3. Clark R. L., Marr M. C., Schwab J. H., Cromartie W. J. Microangiographic studies of experimental erosive synovitis in rats. Invest Radiol. 1983 May-Jun;18(3):257–263. doi: 10.1097/00004424-198305000-00008. [DOI] [PubMed] [Google Scholar]
  4. Coligan J. E., Kindt T. J., Krause R. M. Structure of the streptococcal groups A, A-variant and C carbohydrates. Immunochemistry. 1978 Nov;15(10-11):755–760. doi: 10.1016/0161-5890(78)90105-0. [DOI] [PubMed] [Google Scholar]
  5. Cromartie W. J., Craddock J. G., Schwab J. H., Anderle S. K., Yang C. H. Arthritis in rats after systemic injection of streptococcal cells or cell walls. J Exp Med. 1977 Dec 1;146(6):1585–1602. doi: 10.1084/jem.146.6.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dalldorf F. G., Cromartie W. J., Anderle S. K., Clark R. L., Schwab J. H. The relation of experimental arthritis to the distribution of streptococcal cell wall fragments. Am J Pathol. 1980 Aug;100(2):383–402. [PMC free article] [PubMed] [Google Scholar]
  7. David G. S., Reisfeld R. A. Protein iodination with solid state lactoperoxidase. Biochemistry. 1974 Feb 26;13(5):1014–1021. doi: 10.1021/bi00702a028. [DOI] [PubMed] [Google Scholar]
  8. Eisenberg R., Fox A., Greenblatt J. J., Anderle S. K., Cromartie W. J., Schwab J. H. Measurement of bacterial cell wall in tissues by solid-phase radioimmunoassay: correlation of distribution and persistence with experimental arthritis in rats. Infect Immun. 1982 Oct;38(1):127–135. doi: 10.1128/iai.38.1.127-135.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Esser R. E., Stimpson S. A., Cromartie W. J., Schwab J. H. Reactivation of streptococcal cell wall-induced arthritis by homologous and heterologous cell wall polymers. Arthritis Rheum. 1985 Dec;28(12):1402–1411. doi: 10.1002/art.1780281213. [DOI] [PubMed] [Google Scholar]
  10. Fox A., Brown R. R., Anderle S. K., Chetty C., Cromartie W. J., Gooder H., Schwab J. H. Arthropathic properties related to the molecular weight of peptidoglycan-polysaccharide polymers of streptococcal cell walls. Infect Immun. 1982 Mar;35(3):1003–1010. doi: 10.1128/iai.35.3.1003-1010.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gallis H. A., Miller S. E., Wheat R. W. Degradation of 14C-labeled streptococcal cell walls by egg white lysozyme and lysosomal enzymes. Infect Immun. 1976 May;13(5):1459–1466. doi: 10.1128/iai.13.5.1459-1466.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ginsburg I., Lahav M., Giesbrecht P. Effect of leukocyte hydrolases on bacteria XVI. Activation by leukocyte factors and cationic substances of autolytic enzymes in Staphylococcus aureus: modulation by anionic polyelectrolytes in relation to survival of bacteria in inflammatory exudates. Inflammation. 1982 Sep;6(3):269–284. doi: 10.1007/BF00916408. [DOI] [PubMed] [Google Scholar]
  13. Janusz M. J., Chetty C., Eisenberg R. A., Cromartie W. J., Schwab J. H. Treatment of experimental erosive arthritis in rats by injection of the muralytic enzyme mutanolysin. J Exp Med. 1984 Nov 1;160(5):1360–1374. doi: 10.1084/jem.160.5.1360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kandler O., Schleifer K. H., Dandl R. Differentiation of Streptococcus faecalis Andrewes and Horder and Streptococcus faecium Orla-Jensen based on the amino acid composition of their murein. J Bacteriol. 1968 Dec;96(6):1935–1939. doi: 10.1128/jb.96.6.1935-1939.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ohanian S. H., Schwab J. H. Persistence of group a streptococcal cell walls related to chronic inflammation of rabbit dermal connective tissue. J Exp Med. 1967 Jun 1;125(6):1137–1148. doi: 10.1084/jem.125.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schleifer K. H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972 Dec;36(4):407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schwab J. H., Ohanian S. H. Degradation of streptococcal cell wall antigens in vivo. J Bacteriol. 1967 Nov;94(5):1346–1352. doi: 10.1128/jb.94.5.1346-1352.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smialowicz R. J., Schwab J. H. Processing of streptococcal cell walls by rat macrophages and human monocytes in vitro. Infect Immun. 1977 Sep;17(3):591–598. doi: 10.1128/iai.17.3.591-598.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Spitznagel J. K., Goodrum K. J., Warejcka D. J. Rat arthritis due to whole group B streptococci. Clinical and histopathologic features compared with groups A and D. Am J Pathol. 1983 Jul;112(1):37–47. [PMC free article] [PubMed] [Google Scholar]
  20. Stimpson S. A., Brown R. R., Anderle S. K., Klapper D. G., Clark R. L., Cromartie W. J., Schwab J. H. Arthropathic properties of cell wall polymers from normal flora bacteria. Infect Immun. 1986 Jan;51(1):240–249. doi: 10.1128/iai.51.1.240-249.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ulevitch R. J. The preparation and characterization of a radioiodinated bacterial lipopolysaccharide. Immunochemistry. 1978 Mar;15(3):157–164. doi: 10.1016/0161-5890(78)90144-x. [DOI] [PubMed] [Google Scholar]
  22. Westmacott D., Perkins H. R. Effects of lysozyme on Bacillus cereus 569: rupture of chains of bacteria and enhancement of sensitivity to autolysins. J Gen Microbiol. 1979 Nov;115(1):1–11. doi: 10.1099/00221287-115-1-1. [DOI] [PubMed] [Google Scholar]
  23. Wilder R. L., Calandra G. B., Garvin A. J., Wright K. D., Hansen C. T. Strain and sex variation in the susceptibility to streptococcal cell wall-induced polyarthritis in the rat. Arthritis Rheum. 1982 Sep;25(9):1064–1072. doi: 10.1002/art.1780250906. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES