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. 1986 May;52(2):509–512. doi: 10.1128/iai.52.2.509-512.1986

Comparative efficacy of Bacillus anthracis live spore vaccine and protective antigen vaccine against anthrax in the guinea pig.

S F Little, G B Knudson
PMCID: PMC261029  PMID: 3084385

Abstract

Several strains of Bacillus anthracis have been reported previously to cause fatal infection in immunized guinea pigs. In this study, guinea pigs were immunized with either a protective antigen vaccine or a live Sterne strain spore vaccine, then challenged with virulent B. anthracis strains isolated from various host species from the United States and foreign sources. Confirmation of previously reported studies (which used only protective antigen vaccines) was made with the identification of 9 of the 27 challenge isolates as being vaccine resistant. However, guinea pigs immunized with the live Sterne strain spore vaccine were fully protected against these nine isolates. In experiments designed to determine the basis of vaccine resistance, guinea pigs which were immunized with individual toxin components and which demonstrated enzyme-linked immunosorbent assay antibody titers comparable to those induced by Sterne strain vaccine were not protected when challenged with a vaccine-resistant isolate. We concluded that antibodies to toxin components may not be sufficient to provide protection against all strains of B. anthracis and that other antigens may play a role in active immunity. As a practical matter, it follows that the efficacy of anthrax vaccines must be tested by using vaccine-resistant isolates if protection against all possible challenge strains is to be assured.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AUERBACH S., WRIGHT G. G. Studies on immunity in anthrax. VI. Immunizing activity of protective antigen against various strains of Bacillus anthracis. J Immunol. 1955 Aug;75(2):129–133. [PubMed] [Google Scholar]
  2. BEALL F. A., TAYLOR M. J., THORNE C. B. Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis. J Bacteriol. 1962 Jun;83:1274–1280. doi: 10.1128/jb.83.6.1274-1280.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BELTON F. C., DARLOW H. M., HENDERSON D. W. The use of anthrax antigen to immunise man and monkey. Lancet. 1956 Sep 8;271(6941):476–479. doi: 10.1016/s0140-6736(56)91968-7. [DOI] [PubMed] [Google Scholar]
  4. BELTON F. C., STRANGE R. E. Studies on a protective antigen produced in vitro from Bacillus anthracis: medium and methods of production. Br J Exp Pathol. 1954 Apr;35(2):144–152. [PMC free article] [PubMed] [Google Scholar]
  5. Brachman P. S., Gold H., Plotkin S. A., Fekety F. R., Werrin M., Ingraham N. R. Field Evaluation of a Human Anthrax Vaccine. Am J Public Health Nations Health. 1962 Apr;52(4):632–645. doi: 10.2105/ajph.52.4.632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ezzell J. W., Ivins B. E., Leppla S. H. Immunoelectrophoretic analysis, toxicity, and kinetics of in vitro production of the protective antigen and lethal factor components of Bacillus anthracis toxin. Infect Immun. 1984 Sep;45(3):761–767. doi: 10.1128/iai.45.3.761-767.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fish D. C., Mahlandt B. G., Dobbs J. P., Lincoln R. E. Purification and properties of in vitro-produced anthrax toxin components. J Bacteriol. 1968 Mar;95(3):907–918. doi: 10.1128/jb.95.3.907-918.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fubra E. S. Nonproteolytic, avirulent Bacillus anthracis as a live vaccine. J Bacteriol. 1966 Mar;91(3):930–933. doi: 10.1128/jb.91.3.930-933.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green B. D., Battisti L., Koehler T. M., Thorne C. B., Ivins B. E. Demonstration of a capsule plasmid in Bacillus anthracis. Infect Immun. 1985 Aug;49(2):291–297. doi: 10.1128/iai.49.2.291-297.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hambleton P., Carman J. A., Melling J. Anthrax: the disease in relation to vaccines. Vaccine. 1984 Jun;2(2):125–132. doi: 10.1016/0264-410x(84)90003-3. [DOI] [PubMed] [Google Scholar]
  11. JACKSON F. C., WRIGHT G. G., ARMSTRONG J. Immunization of cattle against experimental anthrax with alum-precipitated protective antigen or spore vaccine. Am J Vet Res. 1957 Oct;18(69):771–777. [PubMed] [Google Scholar]
  12. KEPPIE J., HARRIS-SMITH P. W., SMITH H. THE CHEMICAL BASIS OF THE VIRULENCE OF BACILLUS ANTHRACIS. IX. ITS AGGRESSINS AND THEIR MODE OF ACTION. Br J Exp Pathol. 1963 Aug;44:446–453. [PMC free article] [PubMed] [Google Scholar]
  13. KLEIN F., HAINES B. W., MAHLANDT B. G., LINCOLN R. E. IMMUNOLOGIC STUDIES OF ANTHRAX. III. COMPARISON OF ANTIBODY TITER AND IMMUNITY INDEX AFTER ANTHRAX IMMUNIZATION. J Immunol. 1963 Oct;91:431–437. [PubMed] [Google Scholar]
  14. Leppla S. H. Bacillus anthracis calmodulin-dependent adenylate cyclase: chemical and enzymatic properties and interactions with eucaryotic cells. Adv Cyclic Nucleotide Protein Phosphorylation Res. 1984;17:189–198. [PubMed] [Google Scholar]
  15. Mikesell P., Ivins B. E., Ristroph J. D., Dreier T. M. Evidence for plasmid-mediated toxin production in Bacillus anthracis. Infect Immun. 1983 Jan;39(1):371–376. doi: 10.1128/iai.39.1.371-376.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PERSONEUS G., COOPER M. S., PERCIVAL R. C. Studies on an anthrax vaccine prepared from nonencapsulated variants of Bacillus anthracis. Am J Vet Res. 1956 Jan;17(62):153–156. [PubMed] [Google Scholar]
  17. PUZISS M., WRIGHT G. G. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J Bacteriol. 1963 Jan;85:230–236. doi: 10.1128/jb.85.1.230-236.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ristroph J. D., Ivins B. E. Elaboration of Bacillus anthracis antigens in a new, defined culture medium. Infect Immun. 1983 Jan;39(1):483–486. doi: 10.1128/iai.39.1.483-486.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SAWYER W. D., KUEHNE R. W., GOCHENOUR W. S., Jr EFFECT OF EGG YOLK AND PHOSPHATIDES ON ANTHRAX INFECTION OF RATS AND GUINEA PIGS. Proc Soc Exp Biol Med. 1965 Jan;118:105–108. doi: 10.3181/00379727-118-29769. [DOI] [PubMed] [Google Scholar]
  20. SMITH H., GALLOP R. C. The chemical basis of the virulence of Bacillus anthracis. VI. An extracellular immunising aggressin isolated from exudates of infected guinea-pigs. Br J Exp Pathol. 1956 Apr;37(2):144–155. [PMC free article] [PubMed] [Google Scholar]
  21. SMITH N. R., GIBSON T., GORDON R. E., SNEATH P. H. TYPE CULTURES AND PROPOSED NEOTYPE CULTURES OF SOME SPECIES IN THE GENUS BACILLUS. J Gen Microbiol. 1964 Feb;34:269–272. doi: 10.1099/00221287-34-2-269. [DOI] [PubMed] [Google Scholar]
  22. STANLEY J. L., SMITH H. Purification of factor I and recognition of a third factor of the anthrax toxin. J Gen Microbiol. 1961 Sep;26:49–63. doi: 10.1099/00221287-26-1-49. [DOI] [PubMed] [Google Scholar]
  23. Smith H., Stoner H. B. Anthrax toxic complex. Fed Proc. 1967 Sep;26(5):1554–1557. [PubMed] [Google Scholar]
  24. Uchida I., Sekizaki T., Hashimoto K., Terakado N. Association of the encapsulation of Bacillus anthracis with a 60 megadalton plasmid. J Gen Microbiol. 1985 Feb;131(2):363–367. doi: 10.1099/00221287-131-2-363. [DOI] [PubMed] [Google Scholar]
  25. WARD M. K., MCGANN V. G., HOGGE A. L., Jr, HUFF M. L., KANODE R. G., Jr, ROBERTS E. O. STUDIES ON ANTHRAX INFECTIONS IN IMMUNIZED GUINEA PIGS. J Infect Dis. 1965 Feb;115:59–67. doi: 10.1093/infdis/115.1.59. [DOI] [PubMed] [Google Scholar]
  26. WRIGHT G. G., GREEN T. W., KANODE R. G., Jr Studies on immunity in anthrax. V. Immunizing activity of alum-precipitated protective antigen. J Immunol. 1954 Dec;73(6):387–391. [PubMed] [Google Scholar]
  27. WRIGHT G. G., HEDBERG M. A., SLEIN J. B. Studies on immunity in anthrax. III. Elaboration of protective antigen in a chemically defined, non-protein medium. J Immunol. 1954 Apr;72(4):263–269. [PubMed] [Google Scholar]
  28. ZWARTOUW H. T., SMITH H. Polyglutamic acid from Bacillus anthracis grown in vivo; structure and aggressin activity. Biochem J. 1956 Jul;63(3):437–442. doi: 10.1042/bj0630437. [DOI] [PMC free article] [PubMed] [Google Scholar]

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