Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 May;52(2):562–567. doi: 10.1128/iai.52.2.562-567.1986

Immunity to heat-labile enterotoxins of porcine and human Escherichia coli strains achieved with synthetic cholera toxin peptides.

C O Jacob, R Arnon, R A Finkelstein
PMCID: PMC261037  PMID: 3009330

Abstract

Antiserum to a synthetic peptide, CTP3, consisting of residues 50 to 64 of the B subunit of cholera enterotoxin (CT), a sequence which is conserved in heat-labile enterotoxins from Escherichia coli strains of human (H-LT) or porcine (P-LT) origin, cross-reacted with and neutralized all three enterotoxins. It also cross-reacted with genetically engineered chimeric B subunit proteins in which H-LT amino acids had been substituted for the corresponding residues in P-LT. Antisera against another CT peptide, CTP1, consisting of residues 8 to 20, were weakly cross-reactive with H-LT but not with P-LT, whereas antisera against four other CT peptides were not cross-reactive with the heterologous enterotoxins. A single dose of CTP3-linked tetanus toxoid, by itself nonimmunogenic, primed rabbits to a vigorous immune response to subsequent administration of single, small, subimmunizing doses of any of the three enterotoxins.

Full text

PDF
562

Images in this article

564Image
on p.564

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belisle B. W., Twiddy E. M., Holmes R. K. Characterization of monoclonal antibodies to heat-labile enterotoxin encoded by a plasmid from a clinical isolate of Escherichia coli. Infect Immun. 1984 Mar;43(3):1027–1032. doi: 10.1128/iai.43.3.1027-1032.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clements J. D., Finkelstein R. A. Immunological cross-reactivity between a heat-labile enterotoxin(s) of Escherichia coli and subunits of Vibrio cholerae enterotoxin. Infect Immun. 1978 Sep;21(3):1036–1039. doi: 10.1128/iai.21.3.1036-1039.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clements J. D., Finkelstein R. A. Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures. Infect Immun. 1979 Jun;24(3):760–769. doi: 10.1128/iai.24.3.760-769.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dallas W. S., Falkow S. Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin. Nature. 1980 Dec 4;288(5790):499–501. doi: 10.1038/288499a0. [DOI] [PubMed] [Google Scholar]
  5. Finkelstein R. A., Burks M. F., Rieke L. C., McDonald R. J., Browne S. K., Dallas W. S. Application of monoclonal antibodies and genetically-engineered hybrid B-subunit proteins to the analysis of the cholera/coli enterotoxin family. Dev Biol Stand. 1985;59:51–62. [PubMed] [Google Scholar]
  6. Finkelstein R. A., Fujita K., LoSpalluto J. J. Procholeragenoid: an aggregated intermediate in the formation of choleragenoid. J Immunol. 1971 Oct;107(4):1043–1051. [PubMed] [Google Scholar]
  7. Geary S. J., Marchlewicz B. A., Finkelstein R. A. Comparison of heat-labile enterotoxins from porcine and human strains of Escherichia coli. Infect Immun. 1982 Apr;36(1):215–220. doi: 10.1128/iai.36.1.215-220.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harper J. F., Brooker G. Femtomole sensitive radioimmunoassay for cyclic AMP and cyclic GMP after 2'0 acetylation by acetic anhydride in aqueous solution. J Cyclic Nucleotide Res. 1975;1(4):207–218. [PubMed] [Google Scholar]
  9. Holmes R. K., Twiddy E. M. Characterization of monoclonal antibodies that react with unique and cross-reacting determinants of cholera enterotoxin and its subunits. Infect Immun. 1983 Dec;42(3):914–923. doi: 10.1128/iai.42.3.914-923.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holmgren J. Comparison of the tissue receptors for Vibrio cholerae and Escherichia coli enterotoxins by means of gangliosides and natural cholera toxoid. Infect Immun. 1973 Dec;8(6):851–859. doi: 10.1128/iai.8.6.851-859.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacob C. O., Pines M., Arnon R. Neutralization of heat-labile toxin of E. coli by antibodies to synthetic peptides derived from the B subunit of cholera toxin. EMBO J. 1984 Dec 1;3(12):2889–2893. doi: 10.1002/j.1460-2075.1984.tb02226.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jacob C. O., Sela M., Arnon R. Antibodies against synthetic peptides of the B subunit of cholera toxin: crossreaction and neutralization of the toxin. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7611–7615. doi: 10.1073/pnas.80.24.7611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jacob C. O., Sela M., Pines M., Hurwitz S., Arnon R. Both cholera toxin-induced adenylate cyclase activation and cholera toxin biological activity are inhibited by antibodies against related synthetic peptides. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7893–7896. doi: 10.1073/pnas.81.24.7893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Klipstein F. A., Engert R. F., Clements J. D., Houghten R. A. Protection against human and porcine enterotoxigenic strains of Escherichia coli in rats immunized with a cross-linked toxoid vaccine. Infect Immun. 1983 Jun;40(3):924–929. doi: 10.1128/iai.40.3.924-929.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Klipstein F. A., Engert R. F., Clements J. D. Immunization of rats with heat-labile enterotoxin provides uniform protection against heterologous serotypes of enterotoxigenic Escherichia coli. Infect Immun. 1981 Jun;32(3):1100–1104. doi: 10.1128/iai.32.3.1100-1104.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindholm L., Holmgren J., Wikström M., Karlsson U., Andersson K., Lycke N. Monoclonal antibodies to cholera toxin with special reference to cross-reactions with Escherichia coli heat-labile enterotoxin. Infect Immun. 1983 May;40(2):570–576. doi: 10.1128/iai.40.2.570-576.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Marchlewicz B. A., Finkelstein R. A. Immunological differences among the cholera/coli family of enterotoxins. Diagn Microbiol Infect Dis. 1983 Jun;1(2):129–138. doi: 10.1016/0732-8893(83)90042-1. [DOI] [PubMed] [Google Scholar]
  18. Moon H. W., Kohler E. M., Schneider R. A., Whipp S. C. Prevalence of pilus antigens, enterotoxin types, and enteropathogenicity among K88-negative enterotoxigenic Escherichia coli from neonatal pigs. Infect Immun. 1980 Jan;27(1):222–230. doi: 10.1128/iai.27.1.222-230.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moon H. W. Protection against enteric colibacillosis in pigs suckling orally vaccinated dams: evidence for pili as protective antigens. Am J Vet Res. 1981 Feb;42(2):173–177. [PubMed] [Google Scholar]
  20. Morgan R. L., Isaacson R. E., Moon H. W., Brinton C. C., To C. C. Immunization of suckling pigs against enterotoxigenic Escherichia coli-induced diarrheal disease by vaccinating dams with purified 987 or K99 pili: protection correlates with pilus homology of vaccine and challenge. Infect Immun. 1978 Dec;22(3):771–777. doi: 10.1128/iai.22.3.771-777.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Remmers E. F., Colwell R. R., Goldsby R. A. Production and characterization of monoclonal antibodies to cholera toxin. Infect Immun. 1982 Jul;37(1):70–76. doi: 10.1128/iai.37.1.70-76.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Robb M., Nichols J. C., Whoriskey S. K., Murphy J. R. Isolation of hybridoma cell lines and characterization of monoclonal antibodies against cholera enterotoxin and its subunits. Infect Immun. 1982 Oct;38(1):267–272. doi: 10.1128/iai.38.1.267-272.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Söderlind O., Olsson E., Smyth C. J., Möllby R. Effect of parenteral vaccination of dams on intestinal Escherichia coli in piglets with diarrhea. Infect Immun. 1982 Jun;36(3):900–906. doi: 10.1128/iai.36.3.900-906.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yamamoto T., Tamura T., Yokota T. Primary structure of heat-labile enterotoxin produced by Escherichia coli pathogenic for humans. J Biol Chem. 1984 Apr 25;259(8):5037–5044. [PubMed] [Google Scholar]
  26. Zick Y., Cesla R., Shaltiel S. cAMP-dependent protein kinase from mouse thymocytes. Localization, characterization, and evaluation of the physiological relevance of a massive cytosol to nucleus translocation. J Biol Chem. 1979 Feb 10;254(3):879–887. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES