Figure 3.
Western Blot. ET-B protein is expressed in 1. YFP negative cells (i.e. enterocytes and smooth muscle precursors) and 2. YFP-positive ENCCs isolated from the intestines of Ednrb+/− preparations. In contrast the expression of ET-B protein is increased in 3. YFP negative cells but is absent from 4. YFP+ ENCCs from null animals. A 50 Kd marker protein is shown in the rightmost column. Equal amounts of lysate were loaded as measured by staining of ß-actin (not shown). The protein came from cells obtained from preparations ranging in age between E14 to P 7.