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. 1986 May;52(2):628–630. doi: 10.1128/iai.52.2.628-630.1986

Monoclonal antibody to Streptococcus mutans type e cell wall polysaccharide antigen.

H Kato, F Ota, K Fukui, K Yagawa
PMCID: PMC261049  PMID: 3699899

Abstract

A monoclonal antibody against the polysaccharide antigen of Streptococcus mutans serotype e was prepared. It was found that beta-methyl-D-glucopyranoside and cellobiose markedly inhibited the precipitin reaction, whereas maltose showed no inhibition. The beta-glucosyl moiety of the type e polysaccharide seems to be the predominant antigenic determinant of the antigen.

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Selected References

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  1. Ackermans F., Klein J. P., Cormont F., Bazin H., Ogier J. A., Frank R. M., Vreven J. Antibody specificity and antigen characterization of rat monoclonal antibodies against Streptococcus mutans cell wall-associated protein antigens. Infect Immun. 1985 Aug;49(2):344–350. doi: 10.1128/iai.49.2.344-350.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FITZGERALD R. J., KEYES P. H. Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc. 1960 Jul;61:9–19. doi: 10.14219/jada.archive.1960.0138. [DOI] [PubMed] [Google Scholar]
  3. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  4. Gibbons R. J., Houte J. V. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. doi: 10.1146/annurev.mi.29.100175.000315. [DOI] [PubMed] [Google Scholar]
  5. Gustafsson B., Rosén A., Holme T. Monoclonal antibodies against Vibrio cholerae lipopolysaccharide. Infect Immun. 1982 Nov;38(2):449–454. doi: 10.1128/iai.38.2.449-454.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hamada S., Slade H. D. Purification and immunochemical characterization of type e polysaccharide antigen of Streptococcus mutans. Infect Immun. 1976 Jul;14(1):68–76. doi: 10.1128/iai.14.1.68-76.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iacono V. J., Taubman M. A., Smith D. J., Levine M. J. Isolation and immunochemical characterization of the group-specific antigen of Streptococcus mutants 6715. Infect Immun. 1975 Jan;11(1):117–128. doi: 10.1128/iai.11.1.117-128.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Ota F., Kiso M., Okada K., Kato H., Hirota K., Fukui K., Yasuoka M., Ono M., Uegaki K., Morimoto Y. Streptococcus mutans serotype b strain (St. rattus, Coykendall): first isolation in Japan from human dental plaque. Microbiol Immunol. 1985;29(10):1005–1010. doi: 10.1111/j.1348-0421.1985.tb02964.x. [DOI] [PubMed] [Google Scholar]
  11. SOBER H. A., PETERSON E. A. Protein chromatography on ion exchange cellulose. Fed Proc. 1958 Dec;17(4):1116–1126. [PubMed] [Google Scholar]
  12. Scherp H. W. Dental caries: prospects for prevention. Science. 1971 Sep 24;173(4003):1199–1205. doi: 10.1126/science.173.4003.1199. [DOI] [PubMed] [Google Scholar]
  13. Slade H. D. Extraction of Cell-Wall Polysaccharide Antigen from Streptococci. J Bacteriol. 1965 Sep;90(3):667–672. doi: 10.1128/jb.90.3.667-672.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wetherell J. F., Jr, Bleiweis A. S. Antigens of Streptococcus mutans: isolation of a serotype-specific and a cross-reactive antigen from walls of strain V-100 (serotype e). Infect Immun. 1978 Jan;19(1):160–169. doi: 10.1128/iai.19.1.160-169.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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