Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 Jan;51(1):250–253. doi: 10.1128/iai.51.1.250-253.1986

Immunogenic proteins of Trichomonas vaginalis as demonstrated by the immunoblot technique.

G E Garber, E M Proctor, W R Bowie
PMCID: PMC261094  PMID: 3510170

Abstract

Fifteen clinical isolates of Trichomonas vaginalis were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with transfer to nitrocellulose and by immunoblots probed with human sera. All T. vaginalis isolates showed similar banding patterns by Coomassie brilliant blue and silver staining of the electrophoresis gels and by amido black staining of the nitrocellulose. However, by the immunoblot technique, differences in banding patterns were noted, particularly in the high-molecular-weight zone (greater than 1.5 X 10(5], which were consistent in numerous experiments. A common immunogenic band was noted at a molecular weight of approximately 100,000 in all T. vaginalis isolates probed with six sera reactive in an enzyme-linked immunosorbent assay but was not seen or was only faintly visible when isolates were probed with sera considered to be nonreactive by the assay. Many other bands were identified, some of which appeared common to all isolates, but were not recognized by all sera tested. These studies demonstrate the antigenic heterogeneity of T. vaginalis and show that different individuals appear to respond immunologically to different T. vaginalis antigens.

Full text

PDF
250

Images in this article

251Image
on p.251
252Image
on p.252

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackers J. P., Lumsden W. H., Catterall R. D., Coyle R. Antitrichomonal antibody in the vaginal secretions of women infected with T. vaginalis. Br J Vener Dis. 1975 Oct;51(5):319–323. doi: 10.1136/sti.51.5.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alderete J. F. Antigen analysis of several pathogenic strains of Trichomonas vaginalis. Infect Immun. 1983 Mar;39(3):1041–1047. doi: 10.1128/iai.39.3.1041-1047.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alderete J. F. Enzyme linked immunosorbent assay for detecting antibody to Trichomonas vaginalis: use of whole cells and aqueous extract as antigen. Br J Vener Dis. 1984 Jun;60(3):164–170. doi: 10.1136/sti.60.3.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Alderete J. F. Identification of immunogenic and antibody-binding membrane proteins of pathogenic Trichomonas vaginalis. Infect Immun. 1983 Apr;40(1):284–291. doi: 10.1128/iai.40.1.284-291.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown M. T. Trichomoniasis. Practitioner. 1972 Nov;209(253):639–644. [PubMed] [Google Scholar]
  6. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  7. KOTT H., ADLER S. A serological study of Trichomonas sp. parasitic in man. Trans R Soc Trop Med Hyg. 1961 Jul;55:333–344. doi: 10.1016/0035-9203(61)90102-x. [DOI] [PubMed] [Google Scholar]
  8. Kuberski T. Trichomonas vaginalis associated with nongonococcal urethritis and prostatitis. Sex Transm Dis. 1980 Jul-Sep;7(3):135–136. doi: 10.1097/00007435-198007000-00010. [DOI] [PubMed] [Google Scholar]
  9. Mason P. R. Serodiagnosis of Trichomonas vaginalis infection by the indirect fluorescent antibody test. J Clin Pathol. 1979 Dec;32(12):1211–1215. doi: 10.1136/jcp.32.12.1211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Peterson K. M., Alderete J. F. Host plasma proteins on the surface of pathogenic Trichomonas vaginalis. Infect Immun. 1982 Aug;37(2):755–762. doi: 10.1128/iai.37.2.755-762.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Soliman M. A., Ackers J. P., Catterall R. D. Isoenzyme characterisation of Trichomonas vaginalis. Br J Vener Dis. 1982 Aug;58(4):250–256. doi: 10.1136/sti.58.4.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Street D. A., Taylor-Robinson D., Ackers J. P., Hanna N. F., McMillan A. Evaluation of an enzyme-linked immunosorbent assay for the detection of antibody to Trichomonas vaginalis in sera and vaginal secretions. Br J Vener Dis. 1982 Oct;58(5):330–333. doi: 10.1136/sti.58.5.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES