Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1985 Dec;50(3):944–946. doi: 10.1128/iai.50.3.944-946.1985

Detection of the major adhesin P1 in triton shells of virulent Mycoplasma pneumoniae.

I Kahane, S Tucker, D K Leith, J Morrison-Plummer, J B Baseman
PMCID: PMC261177  PMID: 3934082

Abstract

Filamentous structures designated Triton shells were obtained from virulent Mycoplasma pneumoniae by treatment with Triton X-100. Monoclonal antibodies directed against M. pneumoniae were used in conjunction with radioimmunoprecipitation and Western blotting to detect immunologically reactive polypeptides in Triton shells. The major adhesin, protein P1, was associated with these structures.

Full text

PDF
944

Images in this article

945Image
on p.945
945Image
on p.945

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baseman J. B., Cole R. M., Krause D. C., Leith D. K. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol. 1982 Sep;151(3):1514–1522. doi: 10.1128/jb.151.3.1514-1522.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett V., Stenbuck P. J. The membrane attachment protein for spectrin is associated with band 3 in human erythrocyte membranes. Nature. 1979 Aug 9;280(5722):468–473. doi: 10.1038/280468a0. [DOI] [PubMed] [Google Scholar]
  3. Biberfeld G., Biberfeld P. Ultrastructural features of Mycoplasma pneumoniae. J Bacteriol. 1970 Jun;102(3):855–861. doi: 10.1128/jb.102.3.855-861.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collier A. M., Clyde W. A. Relationships Between Mycoplasma pneumoniae and Human Respiratory Epithelium. Infect Immun. 1971 May;3(5):694–701. doi: 10.1128/iai.3.5.694-701.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feldner J., Göbel U., Bredt W. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature. 1982 Aug 19;298(5876):765–767. doi: 10.1038/298765a0. [DOI] [PubMed] [Google Scholar]
  6. Göbel U., Speth V., Bredt W. Filamentous structures in adherent Mycoplasma pneumoniae cells treated with nonionic detergents. J Cell Biol. 1981 Nov;91(2 Pt 1):537–543. doi: 10.1083/jcb.91.2.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  8. Hu P. C., Cole R. M., Huang Y. S., Graham J. A., Gardner D. E., Collier A. M., Clyde W. A., Jr Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science. 1982 Apr 16;216(4543):313–315. doi: 10.1126/science.6801766. [DOI] [PubMed] [Google Scholar]
  9. Hu P. C., Collier A. M., Baseman J. B. Alterations in the metabolism of hamster tracheas in organ culture after infection by virulent Mycoplasma pneumoniae. Infect Immun. 1975 Apr;11(4):704–710. doi: 10.1128/iai.11.4.704-710.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hu P. C., Collier A. M., Baseman J. B. Interaction of virulent Mycoplasma pneumoniae with hamster tracheal organ cultures. Infect Immun. 1976 Jul;14(1):217–224. doi: 10.1128/iai.14.1.217-224.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hu P. C., Collier A. M., Baseman J. B. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J Exp Med. 1977 May 1;145(5):1328–1343. doi: 10.1084/jem.145.5.1328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kahane I., Granek J., Reisch-Saada A. The adhesins of Mycoplasma gallisepticum and M. pneumoniae. Ann Microbiol (Paris) 1984 Jan-Feb;135A(1):25–32. doi: 10.1016/s0769-2609(84)80055-1. [DOI] [PubMed] [Google Scholar]
  13. Krause D. C., Leith D. K., Wilson R. M., Baseman J. B. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect Immun. 1982 Mar;35(3):809–817. doi: 10.1128/iai.35.3.809-817.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Leith D. K., Baseman J. B. Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography. J Bacteriol. 1984 Feb;157(2):678–680. doi: 10.1128/jb.157.2.678-680.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Leith D. K., Trevino L. B., Tully J. G., Senterfit L. B., Baseman J. B. Host discrimination of Mycoplasma pneumoniae proteinaceous immunogens. J Exp Med. 1983 Feb 1;157(2):502–514. doi: 10.1084/jem.157.2.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meng K. E., Pfister R. M. Intracellular structures of Mycoplasma pneumoniae revealed after membrane removal. J Bacteriol. 1980 Oct;144(1):390–399. doi: 10.1128/jb.144.1.390-399.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morrison-Plummer J., Jones D. H., Baseman J. B. An ELISA to detect monoclonal antibodies specific for lipid determinants of Mycoplasma pneumoniae. J Immunol Methods. 1983 Nov 11;64(1-2):165–178. doi: 10.1016/0022-1759(83)90395-2. [DOI] [PubMed] [Google Scholar]
  19. Wall F., Pfister R. M., Somerson N. L. Freeze-fracture confirmation of the presence of a core in the specialized tip structure of Mycoplasma pneumoniae. J Bacteriol. 1983 May;154(2):924–929. doi: 10.1128/jb.154.2.924-929.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wilson M. H., Collier A. M. Ultrastructural study of Mycoplasma pneumoniae in organ culture. J Bacteriol. 1976 Jan;125(1):332–339. doi: 10.1128/jb.125.1.332-339.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES