Figure 5.

Confocal microscopy images of immunostained corneal whole mounts. All eGFP⁺ donor cells (green) expressed varying levels of CD45 (red; colocalized results in yellow). Red only cells are of host origin (arrow). In major histocompatibility complex (MHC) class II stained whole mounts (b) approximately 25% of donor eGFP⁺ cells were MHC class II⁺ (yellow), with host MHC class II⁺ cells (red only) tending to be located in the posterior stroma of the cornea (b; arrows). The eGFP⁺ cells in the corneal stroma were CD11b⁺ (c–e) whereas the eGFP⁺ cells observed in the epithelium were CD11b⁻ and had the characteristic morphology of intraepithelial dendritic cells or Langerhans cells (e; arrows). Scale bars = 40 μm.