Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1985 May;48(2):292–297. doi: 10.1128/iai.48.2.292-297.1985

Adherence of Entamoeba histolytica trophozoites to rat and human colonic mucosa.

J I Ravdin, J E John, L I Johnston, D J Innes, R L Guerrant
PMCID: PMC261303  PMID: 2580787

Abstract

We studied the adherence of [3H]thymidine-labeled axenic Entamoeba histolytica (strain HM1-IMSS) to in vitro preparations of rat and human colonic mucosa. Studies were performed with fixed or unfixed rat colonic mucosa, unfixed rat mucosa exposed to trypsin, unfixed rat submucosa, and fixed human colonic mucosa. Twenty percent of the amebae adhered to fixed rat colonic mucosa; adherence was specifically inhibited by N-acetyl-D-galactosamine (GalNAc), galactose, and asialofetuin. The adherence of amebae to fixed human colonic mucosa was also GalNAc inhibitable. Greater adherence was found with unfixed rat colonic mucosa (40.9%) and was not GalNAc inhibitable unless the tissue was first exposed to trypsin. However, GalNAc did inhibit the adherence of amebae to unfixed rat submucosa. Glutaraldehyde fixation of amebae inactivates known amebic adhesion proteins; there was a markedly decreased adherence of fixed amebae to trypsin-exposed mucosa or fixed rat colonic mucosa. However, fixed or viable amebae had equal levels of adherence to unfixed rat colonic mucosa, suggesting the presence of a host adhesion protein that binds to receptors on amebae. Human (10%) and rabbit (5%) immune sera reduced the adherence of viable amebae to fixed rat colonic mucosa. We concluded that the GalNAc-inhibitable adhesion protein on the surface of E. histolytica trophozoites mediated adherence to fixed rat mucosa, fixed human colonic mucosa, trypsin-exposed unfixed rat mucosa, and unfixed rat submucosa. The surface of unfixed rat colonic mucosa contained a glutaraldehyde- and trypsin-sensitive host adhesion protein, perhaps in the overlying mucus blanket, which bound viable or fixed E. histolytica trophozoites.

Full text

PDF
292

Images in this article

294Image
on p.294

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aust-Kettis A., Sundqvist K. G. Dynamics of the interaction between Entamoeba histolytica and components of the immune response. I. Capping and endocytosis; influence of inhibiting and accelerating factors; variation of the expression of surface antigens. Scand J Immunol. 1978;7(1):35–44. doi: 10.1111/j.1365-3083.1978.tb00424.x. [DOI] [PubMed] [Google Scholar]
  2. Boland C. R., Montgomery C. K., Kim Y. S. Alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2051–2055. doi: 10.1073/pnas.79.6.2051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bracha R., Kobiler D., Mirelman D. Attachment and ingestion of bacteria by trophozoites of Entamoeba histolytica. Infect Immun. 1982 Apr;36(1):396–406. doi: 10.1128/iai.36.1.396-406.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bracha R., Mirelman D. Adherence and ingestion of Escherichia coli serotype 055 by trophozoites of Entamoeba histolytica. Infect Immun. 1983 Jun;40(3):882–887. doi: 10.1128/iai.40.3.882-887.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Calderón J., de Lourdes Muñoz M., Acosta H. M. Surface redistribution and release of antibody-induced caps in entamoebae. J Exp Med. 1980 Jan 1;151(1):184–193. doi: 10.1084/jem.151.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  7. Essner E., Schreiber J., Griewski R. A. Localization of carbohydrate components in rat colon with fluoresceinated lectins. J Histochem Cytochem. 1978 Jun;26(6):452–458. doi: 10.1177/26.6.670681. [DOI] [PubMed] [Google Scholar]
  8. Guerrant R. L., Brush J., Ravdin J. I., Sullivan J. A., Mandell G. L. Interaction between Entamoeba histolytica and human polymorphonuclear neutrophils. J Infect Dis. 1981 Jan;143(1):83–93. doi: 10.1093/infdis/143.1.83. [DOI] [PubMed] [Google Scholar]
  9. Kobiler D., Mirelman D. Lectin activity in Entamoeba histolytica trophozoites. Infect Immun. 1980 Jul;29(1):221–225. doi: 10.1128/iai.29.1.221-225.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leitch G. J., Dickey A. D., Udezulu I. A., Bailey G. B. Entamoeba histolytica trophozoites in the lumen and mucus blanket of rat colons studied in vivo. Infect Immun. 1985 Jan;47(1):68–73. doi: 10.1128/iai.47.1.68-73.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Levy D. A., Frondoza C. Immunity to intestinal parasites: role of mast cells and goblet cells. Fed Proc. 1983 Apr;42(6):1750–1755. [PubMed] [Google Scholar]
  12. Lushbaugh W. B., Kairalla A. B., Cantey J. R., Hofbauer A. F., Pittman F. E. Isolation of a cytotoxin-enterotoxin from Entamoeba histolytica. J Infect Dis. 1979 Jan;139(1):9–17. doi: 10.1093/infdis/139.1.9. [DOI] [PubMed] [Google Scholar]
  13. López-Revilla R., Cano-Mancera R. Adhesion of Entamoeba histolytica trophozoites to human erythrocytes. Infect Immun. 1982 Jul;37(1):281–285. doi: 10.1128/iai.37.1.281-285.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mahajan R. C., Agarwal S. C., Chhuttani P. N., Chitkara N. L. Coproantibodies in intestinal amoebiasis. Indian J Med Res. 1972 Apr;60(4):547–550. [PubMed] [Google Scholar]
  15. McGowan K., Deneke C. F., Thorne G. M., Gorbach S. L. Entamoeba histolytica cytotoxin: purification, characterization, strain virulence, and protease activity. J Infect Dis. 1982 Nov;146(5):616–625. doi: 10.1093/infdis/146.5.616. [DOI] [PubMed] [Google Scholar]
  16. Mora Galindo J., Martinez-Palomo A., Chavez B. Interacción entre Entamoeba histolytica y el epitelio cecal del cobayo. Arch Invest Med (Mex) 1978;9 (Suppl 1):261–274. [PubMed] [Google Scholar]
  17. Muńoz M. L., Calderón J., Rojkind M. The collagenase of Entamoeba histolytica. J Exp Med. 1982 Jan 1;155(1):42–51. doi: 10.1084/jem.155.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Orozco M. E., Martínez Palomo A., González Robles A., Guarneros G., Galindo J. M. Las interacciones entre lectina y receptor median la adherencia de E. histolytica a células epiteliales. Relación de la adhesión con la virulencia de las cepas. Arch Invest Med (Mex) 1982;13 (Suppl 3):159–167. [PubMed] [Google Scholar]
  19. Podolsky D. K., Isselbacher K. J. Composition of human colonic mucin. Selective alteration in inflammatory bowel disease. J Clin Invest. 1983 Jul;72(1):142–153. doi: 10.1172/JCI110952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ravdin J. I., Croft B. Y., Guerrant R. L. Cytopathogenic mechanisms of Entamoeba histolytica. J Exp Med. 1980 Aug 1;152(2):377–390. doi: 10.1084/jem.152.2.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ravdin J. I., Guerrant R. L. Role of adherence in cytopathogenic mechanisms of Entamoeba histolytica. Study with mammalian tissue culture cells and human erythrocytes. J Clin Invest. 1981 Nov;68(5):1305–1313. doi: 10.1172/JCI110377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ross G. W., Knight R. Dietary factors affecting the pathogenicity of Entamoeba histolytica in rats. Trans R Soc Trop Med Hyg. 1973;67(4):560–567. doi: 10.1016/0035-9203(73)90087-4. [DOI] [PubMed] [Google Scholar]
  23. Shaalan M., Baker R. P. Detection of coproantibodies in amebiasis of the colon: a preliminary report. Am J Clin Pathol. 1970 Oct;54(4):615–617. doi: 10.1093/ajcp/54.4.615. [DOI] [PubMed] [Google Scholar]
  24. Singh B. N., Srivastava R. V., Dutta G. P. Virulence of strains of Entamoeba histolytica to rats and the effect of cholesterol, rat caecal and hamster liver passage on the virulence of non-invasive strains. Indian J Exp Biol. 1971 Jan;9(1):21–27. [PubMed] [Google Scholar]
  25. Takeuchi A., Phillips B. P. Electron microscope studies of experimental Entamoeba histolytica infection in the guinea pig. I. Penetration of the intestinal epithelium by trophozoites. Am J Trop Med Hyg. 1975 Jan;24(1):34–48. doi: 10.4269/ajtmh.1975.24.34. [DOI] [PubMed] [Google Scholar]
  26. Tsutsumi V., Mena-Lopez R., Anaya-Velazquez F., Martinez-Palomo A. Cellular bases of experimental amebic liver abscess formation. Am J Pathol. 1984 Oct;117(1):81–91. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES