Figure 4.

qPCR confirms regulation of multiple genes by STX in the arcuate nucleus. The relative mRNA expression for selected control genes (A and B) and selected genes of interest (C and D) from microdissected arcuate tissue from vehicle-treated [propylene glycol (n = 5) or oil (n = 7); black bars] females and STX- (n = 7) or EB-treated (n = 6) females (gray bars). The expression values were calculated using the ΔΔCT method where the calibrator was the average ΔCT of the vehicle-treated samples. Bar graphs represent the mean ± sem. Statistics: two-tailed Student’s t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with vehicle).