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. 1985 Mar;47(3):648–653. doi: 10.1128/iai.47.3.648-653.1985

Role of nasopharyngeal colonization with and without bacteremia in the protection of infant rats against Haemophilus influenzae type b challenge.

J R Gilsdorf, P Ferrieri
PMCID: PMC261345  PMID: 3871734

Abstract

Nasopharyngeal colonization of infant rats with Haemophilus influenzae type b was investigated by two methods of intranasal inoculation. After traumatic instillation of the bacteria, 100% of the animals became colonized, compared with 75.5% of animals after atraumatic instillation. Among colonized rats, significantly more animals in the traumatic group developed bacteremia compared with those in the atraumatic group. Rats in the traumatic group had an onset of bacteremia at a mean of 2.6 days after inoculation compared with 7.3 days in the atraumatic group. The duration of colonization and bacteremia was the same in both groups. The majority of heavily colonized rats developed bacteremia compared with none of the lightly colonized rats. Thus, the development of bacteremia appeared to be related independently to both heavy colonization and traumatic instillation. Protection against intraperitoneal bacterial challenge with H. influenzae type b developed in rats that had been bacteremic; in the majority of animals, this correlated with the development of serum bactericidal activity. Protection and bactericidal activity were only rarely observed in nonbacteremic rats that had been either heavily or lightly colonized. The development of serum bactericidal activity was not related to either the duration or peak level of bacteremia. Thus, in this rat model, H. influenzae type b nasopharyngeal colonization without bacteremia did not appear to stimulate circulating antibodies that protected the animals against challenge.

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Selected References

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  1. Anderson P., Johnston R. B., Jr, Smith D. H. Human serum activities against Hemophilus influenzae, type b. J Clin Invest. 1972 Jan;51(1):31–38. doi: 10.1172/JCI106793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradshaw M. W., Schneerson R., Parke J. C., Jr, Robbins J. B. Bacterial antigens cross-reactive with the capsular polysaccharide of Haemophilus influenzae type b. Lancet. 1971 May 29;1(7709):1095–1096. doi: 10.1016/s0140-6736(71)91837-x. [DOI] [PubMed] [Google Scholar]
  3. Gigliotti F., Insel R. A. Protection from infection with Haemophilus influenzae type b by monoclonal antibody to the capsule. J Infect Dis. 1982 Aug;146(2):249–254. doi: 10.1093/infdis/146.2.249. [DOI] [PubMed] [Google Scholar]
  4. Ginsburg C. M., McCracken G. H., Jr, Schneerson R., Robbins J. B., Parke J. C., Jr Association between cross-reacting Escherichia coli K100 and disease caused by Haemophilus influenzae type b. Infect Immun. 1978 Nov;22(2):339–342. doi: 10.1128/iai.22.2.339-342.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Granoff D. M., Gilsdorf J., Gessert C. E., Lowe L. Haemophilus influenzae type b in a day care center: relationship of nasopharyngeal carriage to development of anticapsular antibody. Pediatrics. 1980 Jan;65(1):65–68. [PubMed] [Google Scholar]
  6. Granoff D. M., Rockwell R. Experimental Haemophilus influenzae type b meningitis: immunological investigation of the infant rat model. Infect Immun. 1978 Jun;20(3):705–713. doi: 10.1128/iai.20.3.705-713.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gray B. M., Converse G. M., 3rd, Huhta N., Johnston R. B., Jr, Pichichero M. E., Schiffman G., Dillon H. C., Jr Epidemiologic studies of Streptococcus pneumoniae in infants: antibody response to nasopharyngeal carriage of types 3, 19, and 23. J Infect Dis. 1981 Oct;144(4):312–318. doi: 10.1093/infdis/144.4.312. [DOI] [PubMed] [Google Scholar]
  8. Greenfield S., Peter G., Howie V. M., Ploussard J. H., Smith D. H. Acquisition of type-specific antibodies to Hemophilus influenzae type b. J Pediatr. 1972 Feb;80(2):204–208. doi: 10.1016/s0022-3476(72)80579-1. [DOI] [PubMed] [Google Scholar]
  9. Halsey N. A., Korock C., Johansen T. L., Glode M. P. Intralitter transmission of haemophilus influenzae type b in infant rats and rifampin eradication of nasopharyngeal colonization. J Infect Dis. 1980 Nov;142(5):739–743. doi: 10.1093/infdis/142.5.739. [DOI] [PubMed] [Google Scholar]
  10. Michaels R. H., Norden C. W. Pharyngeal colonization with Haemophilus influenzae type b: a longitudinal study of families with a child with meningitis or epiglottitis due to H. influenzae type b. J Infect Dis. 1977 Aug;136(2):222–228. doi: 10.1093/infdis/136.2.222. [DOI] [PubMed] [Google Scholar]
  11. Michaels R. H., Stonebraker F. E., Robbins J. B. Use of antiserum agar for detection of Haemophilus influenzae type b in the pharynx. Pediatr Res. 1975 May;9(5):513–516. doi: 10.1203/00006450-197505000-00010. [DOI] [PubMed] [Google Scholar]
  12. Moxon E. R., Anderson P. Meningitis caused by Haemophilus influenzae in infant rats: protective immunity and antibody priming by gastrointestinal colonization with Escherichia coli. J Infect Dis. 1979 Oct;140(4):471–478. doi: 10.1093/infdis/140.4.471. [DOI] [PubMed] [Google Scholar]
  13. Moxon E. R., Murphy P. A. Haemophilus influenzae bacteremia and meningitis resulting from survival of a single organism. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1534–1536. doi: 10.1073/pnas.75.3.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moxon E. R., Smith A. L., Averill D. R., Smith D. H. Haemophilus influenzae meningitis in infant rats after intranasal inoculation. J Infect Dis. 1974 Feb;129(2):154–162. doi: 10.1093/infdis/129.2.154. [DOI] [PubMed] [Google Scholar]
  15. Myerowitz R. L., Michaels R. H. Mechanism of potentiation of experimental Haemophilus influenzae type B disease in infant rats by influenza A virus. Lab Invest. 1981 May;44(5):434–441. [PubMed] [Google Scholar]
  16. Myerowitz R. L., Norden C. W. Effect of neonatal gastrointestinal colonization with cross reacting Escherichia coli on anticapsular antibody production and bacteremia in experimental Haemophilus influenzae type b disease of rats. Infect Immun. 1977 Jul;17(1):83–90. doi: 10.1128/iai.17.1.83-90.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Norden C. W., Feldman H. A. Hemophilus influenzae, type b, antibody frequencies determined with bactericidal and radioimmunoassay tests. J Clin Microbiol. 1976 Aug;2(2):136–138. [PMC free article] [PubMed] [Google Scholar]
  18. Peltola H., Käyhty H., Sivonen A., Mäkelä H. Haemophilus influenzae type b capsular polysaccharide vaccine in children: a double-blind field study of 100,000 vaccinees 3 months to 5 years of age in Finland. Pediatrics. 1977 Nov;60(5):730–737. [PubMed] [Google Scholar]
  19. Rubin L. G., Moxon E. R. Pathogenesis of bloodstream invasion with Haemophilus influenzae type b. Infect Immun. 1983 Jul;41(1):280–284. doi: 10.1128/iai.41.1.280-284.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schneerson R., Robbins J. B. Induction of serum Haemophilus influenzae type B capsular antibodies in adult volunteers fed cross-reacting Escherichia coli 075:K100:H5. N Engl J Med. 1975 May 22;292(21):1093–1096. doi: 10.1056/NEJM197505222922103. [DOI] [PubMed] [Google Scholar]
  21. Smith A. L., Smith D. H., Averill D. R., Jr, Marino J., Moxon E. R. Production of Haemophilus influenzae b meningitis in infant rats by intraperitoneal inoculation. Infect Immun. 1973 Aug;8(2):278–290. doi: 10.1128/iai.8.2.278-290.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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