Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1985 Mar;47(3):752–759. doi: 10.1128/iai.47.3.752-759.1985

Surface properties of Streptococcus sanguis FW213 mutants nonadherent to saliva-coated hydroxyapatite.

P M Fives-Taylor, D W Thompson
PMCID: PMC261379  PMID: 2857684

Abstract

Seventeen mutants of Streptococcus sanguis FW213 nonadherent to saliva-coated spheroidal hydroxyapatite were isolated after mutagenesis with ethyl methanesulfonate, nitrosoguanidine, nitrous acid, hydroxylamine, or 2-aminopurine. Enrichment for nonadherent mutants was accomplished by successive adsorptions of the adherent strains to saliva-coated hydroxyapatite. After enrichment, variant colonial morphology on tryptic agar was used as a screening technique for selection of nonadherent mutants, with loss of colonial opacity frequently associated with loss of adherence ability. These mutants were further characterized for additional surface properties, including twitching motility, saliva-induced aggregation, coaggregation with Actinomyces species, surface hydrophobicity, and presence of fimbriae. Results from these assays indicated that the nonadherent mutants fell into six phenotypic groups. A correlation between the loss of adherence ability, a decrease in cell fimbriation, and a decrease in surface hydrophobicity is apparent.

Full text

PDF
752

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. doi: 10.1093/infdis/143.3.325. [DOI] [PubMed] [Google Scholar]
  2. Carlsson J., Grahnén H., Jonsson G., Wikner S. Establishment of Streptococcus sanguis in the mouths of infants. Arch Oral Biol. 1970 Dec;15(12):1143–1148. doi: 10.1016/0003-9969(70)90005-1. [DOI] [PubMed] [Google Scholar]
  3. Cisar J. O., Kolenbrander P. E., McIntire F. C. Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. Infect Immun. 1979 Jun;24(3):742–752. doi: 10.1128/iai.24.3.742-752.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cole R. M., Calandra G. B., Huff E., Nugent K. M. Attributes of potential utility in differentiating among "group H" streptococci or Streptococcus sanguis. J Dent Res. 1976 Jan;55:A142–A153. doi: 10.1177/002203457605500106011. [DOI] [PubMed] [Google Scholar]
  5. Edén C. S., Hansson H. A. Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect Immun. 1978 Jul;21(1):229–237. doi: 10.1128/iai.21.1.229-237.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elder B. L., Boraker D. K., Fives-Taylor P. M. Whole-bacterial cell enzyme-linked immunosorbent assay for Streptococcus sanguis fimbrial antigens. J Clin Microbiol. 1982 Jul;16(1):141–144. doi: 10.1128/jcm.16.1.141-144.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ellen R. P., Gibbons R. J. Parameters affecting the adherence and tissue tropisms of Streptococcus pyogenes. Infect Immun. 1974 Jan;9(1):85–91. doi: 10.1128/iai.9.1.85-91.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gibbons R. J., Etherden I., Skobe Z. Association of fimbriae with the hydrophobicity of Streptococcus sanguis FC-1 and adherence to salivary pellicles. Infect Immun. 1983 Jul;41(1):414–417. doi: 10.1128/iai.41.1.414-417.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gibbons R. J., Moreno E. C., Etherden I. Concentration-dependent multiple binding sites on saliva-treated hydroxyapatite for Streptococcus sanguis. Infect Immun. 1983 Jan;39(1):280–289. doi: 10.1128/iai.39.1.280-289.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gibbons R. J., Moreno E. C., Spinell D. M. Model delineating the effects of a salivary pellicle on the adsorption of Streptococcus miteor onto hydroxyapatite. Infect Immun. 1976 Oct;14(4):1109–1112. doi: 10.1128/iai.14.4.1109-1112.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Henriksen S. D., Henrichsen J. Twitching motility and possession of polar fimbriae in spreading Streptococcus sanguis isolates from the human throat. Acta Pathol Microbiol Scand B. 1975 Apr;83(2):133–140. doi: 10.1111/j.1699-0463.1975.tb00083.x. [DOI] [PubMed] [Google Scholar]
  12. Hillman J. D., Van Houte J., Gibbons R. J. Sorption of bacteria to human enamel powder. Arch Oral Biol. 1970 Sep;15(9):899–903. doi: 10.1016/0003-9969(70)90163-9. [DOI] [PubMed] [Google Scholar]
  13. KAUDEWITZ F. Production of bacterial mutants with nitrous acid. Nature. 1959 Jun 27;183:1829–1830. doi: 10.1038/1831829a0. [DOI] [PubMed] [Google Scholar]
  14. LIE S. THE MUTAGENIC EFFECT OF HYDROXYLAMINE ON ESCHERICHIA COLI. Acta Pathol Microbiol Scand. 1964;62:575–580. doi: 10.1111/apm.1964.62.4.575. [DOI] [PubMed] [Google Scholar]
  15. LOVELESS A., HOWARTH S. Mutation of bacteria at high levels of survival by ethyl methane sulphonate. Nature. 1959 Dec 5;184:1780–1782. doi: 10.1038/1841780a0. [DOI] [PubMed] [Google Scholar]
  16. Liljemark W. F., Bloomquist C. G. Isolation of a protein-containing cell surface component from Streptococcus sanguis which affects its adherence to saliva-coated hydroxyapatite. Infect Immun. 1981 Nov;34(2):428–434. doi: 10.1128/iai.34.2.428-434.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Liljemark W. F., Schauer S. V. Studies on the bacterial components which bind Streptococcus sanguis and Streptococcus mutans to hydroxyapatite. Arch Oral Biol. 1975 Sep;20(9):609–615. doi: 10.1016/0003-9969(75)90082-5. [DOI] [PubMed] [Google Scholar]
  18. Lindahl M., Faris A., Wadström T., Hjertén S. A new test based on 'salting out' to measure relative surface hydrophobicity of bacterial cells. Biochim Biophys Acta. 1981 Nov 5;677(3-4):471–476. doi: 10.1016/0304-4165(81)90261-0. [DOI] [PubMed] [Google Scholar]
  19. McBride B. C., Gisslow M. T. Role of sialic acid in saliva-induced aggregation of Streptococcus sanguis. Infect Immun. 1977 Oct;18(1):35–40. doi: 10.1128/iai.18.1.35-40.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morris E. J., McBride B. C. Adherence of Streptococcus sanguis to saliva-coated hydroxyapatite: evidence for two binding sites. Infect Immun. 1984 Feb;43(2):656–663. doi: 10.1128/iai.43.2.656-663.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Murchison H., Larrimore S., Curtiss R., 3rd Isolation and characterization of Streptococcus mutans mutants defective in adherence and aggregation. Infect Immun. 1981 Dec;34(3):1044–1055. doi: 10.1128/iai.34.3.1044-1055.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nesbitt W. E., Doyle R. J., Taylor K. G. Hydrophobic interactions and the adherence of Streptococcus sanguis to hydroxylapatite. Infect Immun. 1982 Nov;38(2):637–644. doi: 10.1128/iai.38.2.637-644.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rosan B., Malamud D., Appelbaum B., Golub E. Characteristic differences between saliva-dependent aggregation and adhesion of streptococci. Infect Immun. 1982 Jan;35(1):86–90. doi: 10.1128/iai.35.1.86-90.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rölla G., Robrish S. A., Bowen W. H. Interaction of hydroxyapatite and protein-coated hydroxyapatite with Streptococcus mutans and Streptococcus sanguis. Acta Pathol Microbiol Scand B. 1977 Oct;85B(5):341–346. doi: 10.1111/j.1699-0463.1977.tb01985.x. [DOI] [PubMed] [Google Scholar]
  25. Swaney L. M., Liu Y. P., To C. M., To C. C., Ippen-Ihler K., Brinton C. C., Jr Isolation and characterization of Escherichia coli phase variants and mutants deficient in type 1 pilus production. J Bacteriol. 1977 Apr;130(1):495–505. doi: 10.1128/jb.130.1.495-505.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Swanson J. Studies on gonococcus infection. XIV. Cell wall protein differences among color/opacity colony variants of Neisseria gonorrhoeae. Infect Immun. 1978 Jul;21(1):292–302. doi: 10.1128/iai.21.1.292-302.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES