Table 5.
PolyP content in E. coli expressing wild-type and mutated rPPK
| Protein expressed | PolyP content,* nmol/mg of cell protein |
|---|---|
| rPPK | 330 |
| H430Q | 311 |
| H441Q | 0.29 |
| H460Q | 0.17 |
| H598Q | 220 |
| H441A | 0.08 |
| H460A | 0.08 |
As Pi residues
PolyP content in E. coli expressing wild-type and mutated rPPK
| Protein expressed | PolyP content,* nmol/mg of cell protein |
|---|---|
| rPPK | 330 |
| H430Q | 311 |
| H441Q | 0.29 |
| H460Q | 0.17 |
| H598Q | 220 |
| H441A | 0.08 |
| H460A | 0.08 |
As Pi residues