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. 1984 Oct;46(1):64–67. doi: 10.1128/iai.46.1.64-67.1984

Adoptive transfer of immune enhancement of experimental ulcerative colitis.

A B Onderdonk, R M Steeves, R L Cisneros, R T Bronson
PMCID: PMC261421  PMID: 6480115

Abstract

Previous experiments with the carrageenan model for ulcerative colitis have shown that the inflammatory response in guinea pigs can be enhanced by immunization with and subsequent feeding of Bacteroides vulgatus to experimental animals. The present studies showed that only certain strains of B. vulgatus are capable of provoking immune enhancement of ulcerative colitis. Animals were fed carrageenan and various strains of viable B. vulgatus after immunization with a strain of B. vulgatus isolated from a guinea pig with experimentally induced colitis. Histological comparison of immune and nonimmune groups revealed that immune animals which received B. vulgatus from a patient with inflammatory bowel disease had a significantly (P less than 0.025) greater number of histopathological lesions at 21 days than did nonimmune animals. Immune animals receiving B. vulgatus isolated from a clinically normal source did not show any significant difference in disease status when compared to nonimmune animals. Additional experiments showed that adoptive transfer of spleen cells from animals immunized with B. vulgatus to nonimmune recipient animals is effective in transferring the immune enhancement demonstrated in actively immunized animals. Animals which received immune spleen cells with concurrent feeding of B. vulgatus showed a significant (P less than 0.005) increase in inflammation over control groups, in the absence of high titers of circulating antibody. These experiments indicate that B. vulgatus strain-specific factors are important to immune enhancement of experimental disease and also suggest an involvement of the cell-mediated immune system in this model.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bendixen G. Cellular hypersensitivity to components of intestinal mucosa in ulcerative colitis and Crohn's disease. Gut. 1969 Aug;10(8):631–636. doi: 10.1136/gut.10.8.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lambe D. W., Jr, Moroz D. A. Serogrouping of Bacteroides fragilis subsp. fragilis by the agglutination test. J Clin Microbiol. 1976 Jun;3(6):586–592. doi: 10.1128/jcm.3.6.586-592.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Monteiro E., Fossey J., Shiner M., Drasar B. S., Allison A. C. Antibacterial antibodies in rectal and colonic mucosa in ulcerative colitis. Lancet. 1971 Feb 6;1(7693):249–250. doi: 10.1016/s0140-6736(71)90997-4. [DOI] [PubMed] [Google Scholar]
  4. Moolenbeek C., Ruitenberg E. J. The "Swiss roll": a simple technique for histological studies of the rodent intestine. Lab Anim. 1981 Jan;15(1):57–59. doi: 10.1258/002367781780958577. [DOI] [PubMed] [Google Scholar]
  5. Onderdonk A. B., Bartlett J. G. Bacteriological studies of experimental ulcerative colitis. Am J Clin Nutr. 1979 Jan;32(1):258–265. doi: 10.1093/ajcn/32.1.258. [DOI] [PubMed] [Google Scholar]
  6. Onderdonk A. B., Cisneros R. L., Bronson R. T. Enhancement of experimental ulcerative colitis by immunization with Bacteroides vulgatus. Infect Immun. 1983 Nov;42(2):783–788. doi: 10.1128/iai.42.2.783-788.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Onderdonk A. B., Franklin M. L., Cisneros R. L. Production of experimental ulcerative colitis in gnotobiotic guinea pigs with simplified microflora. Infect Immun. 1981 Apr;32(1):225–231. doi: 10.1128/iai.32.1.225-231.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Onderdonk A. B., Hermos J. A., Bartlett J. G. The role of the intestinal microflora in experimental colitis. Am J Clin Nutr. 1977 Nov;30(11):1819–1825. doi: 10.1093/ajcn/30.11.1819. [DOI] [PubMed] [Google Scholar]
  9. Onderdonk A. B., Hermos J. A., Dzink J. L., Bartlett J. G. Protective effect of metronidazole in experimental ulcerative colitis. Gastroenterology. 1978 Mar;74(3):521–526. [PubMed] [Google Scholar]
  10. Rabin B. S., Rogers S. J. A cell-mediated immune model of inflammatory bowel disease in the rabbit. Gastroenterology. 1978 Jul;75(1):29–33. [PubMed] [Google Scholar]
  11. Watt J., Marcus R. Carrageenan-induced ulceration of the large intestine in the guinea pig. Gut. 1971 Feb;12(2):164–171. doi: 10.1136/gut.12.2.164. [DOI] [PMC free article] [PubMed] [Google Scholar]

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