Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1984 Oct;46(1):245–250. doi: 10.1128/iai.46.1.245-250.1984

Cell surface proteins of oral streptococci.

B Appelbaum, B Rosan
PMCID: PMC261463  PMID: 6480109

Abstract

Whole cells of representative strains of oral streptococci (Streptococcus sanguis, S. mitis, and S. salivarius) were radiolabeled by the lactoperoxidase method of radioiodination. The labeled polypeptides obtained by extraction of whole cells with boiling sodium dodecyl sulfate were analyzed by polyacrylamide gel electrophoresis and autoradiography. Of the total radioactivity, ca. 70% was released by treating whole cells with trypsin, suggesting that the labeling was confined to proteins located on the cell surface. Most S. sanguis strains studied gave a characteristic banding pattern consisting of a high-molecular-weight (120,000 [120K] to 63K) group of six proteins. Three low-molecular-weight (12K, 16K, and 18K) proteins were also detected in many strains.

Full text

PDF
245

Images in this article

246Image
on p.246
247Image
on p.247
247Image
on p.247
247Image
on p.247
248Image
on p.248
248Image
on p.248
249Image
on p.249

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum B., Golub E., Holt S. C., Rosan B. In vitro studies of dental plaque formation: adsorption of oral streptococci to hydroxyaptite. Infect Immun. 1979 Aug;25(2):717–728. doi: 10.1128/iai.25.2.717-728.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barenkamp S. J., Munson R. S., Jr, Granoff D. M. Outer membrane protein and biotype analysis of pathogenic nontypable Haemophilus influenzae. Infect Immun. 1982 May;36(2):535–540. doi: 10.1128/iai.36.2.535-540.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Björck L., Kronvall G. Analysis of bacterial cell wall proteins and human serum proteins bound to bacterial cell surfaces. Acta Pathol Microbiol Scand B. 1981 Feb;89(1):1–6. doi: 10.1111/j.1699-0463.1981.tb00144_89b.x. [DOI] [PubMed] [Google Scholar]
  4. Booth B. R. Cell surface proteins of E. coli. Biochem Biophys Res Commun. 1980 Jun 30;94(4):1029–1036. doi: 10.1016/0006-291x(80)90522-7. [DOI] [PubMed] [Google Scholar]
  5. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  6. Brener D., DeVoe I. W., Holbein B. E. Increased virulence of Neisseria meningitidis after in vitro iron-limited growth at low pH. Infect Immun. 1981 Jul;33(1):59–66. doi: 10.1128/iai.33.1.59-66.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carlsson J., Grahnén H., Jonsson G., Wikner S. Establishment of Streptococcus sanguis in the mouths of infants. Arch Oral Biol. 1970 Dec;15(12):1143–1148. doi: 10.1016/0003-9969(70)90005-1. [DOI] [PubMed] [Google Scholar]
  8. Coykendall A. L., Specht P. A. DNA base sequence homologies among strains of Streptococcus sanguis. J Gen Microbiol. 1975 Nov;91(1):92–98. doi: 10.1099/00221287-91-1-92. [DOI] [PubMed] [Google Scholar]
  9. Di Rienzo J. M., Spieler E. L. Identification and characterization of the major cell envelope proteins of oral strains of Actinobacillus actinomycetemcomitans. Infect Immun. 1983 Jan;39(1):253–261. doi: 10.1128/iai.39.1.253-261.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Douglas C. W., Russell R. R. Effect of specific antisera on adherence properties of the oral bacterium Streptococcus mutans. Arch Oral Biol. 1982;27(12):1039–1045. doi: 10.1016/0003-9969(82)90009-7. [DOI] [PubMed] [Google Scholar]
  11. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  12. Golub E. E., Thaler M., Davis C., Malamud D. Bacterial aggregating activity in human saliva: simultaneous determination of free and bound cells. Infect Immun. 1979 Dec;26(3):1028–1034. doi: 10.1128/iai.26.3.1028-1034.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hamada S., Mizuno J. Isoelectric focusing in polyacrylamide gel of the membrane proteins of Streptococcus mutans and related streptococci. J Dent Res. 1974 May-Jun;53(3):547–553. doi: 10.1177/00220345740530030601. [DOI] [PubMed] [Google Scholar]
  14. Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hansen E. J., Frisch C. F., Johnston K. H. Detection of antibody-accessible proteins on the cell surface of Haemophilus influenzae type b. Infect Immun. 1981 Sep;33(3):950–953. doi: 10.1128/iai.33.3.950-953.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jones S. J. A special relationship between spherical and filamentous microorganisms in mature human dental plaque. Arch Oral Biol. 1972 Mar;17(3):613–616. doi: 10.1016/0003-9969(72)90081-7. [DOI] [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Lancy P., Jr, Appelbaum B., Holt S. C., Rosan B. Quantitative in vitro assay for "corncob" formation. Infect Immun. 1980 Aug;29(2):663–670. doi: 10.1128/iai.29.2.663-670.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Larsen S. A., Webb C. D., Moody M. D. Acrylamide gel electrophoresis of group A streptococcal cell walls. Appl Microbiol. 1969 Jan;17(1):31–33. doi: 10.1128/am.17.1.31-33.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Liljemark W. F., Bloomquist C. G. Isolation of a protein-containing cell surface component from Streptococcus sanguis which affects its adherence to saliva-coated hydroxyapatite. Infect Immun. 1981 Nov;34(2):428–434. doi: 10.1128/iai.34.2.428-434.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Loeb M. R., Smith D. H. Lactoperoxidase and Iodo-Gen-catalyzed iodination labels inner and outer membrane proteins of Haemophilus influenzae. J Bacteriol. 1983 Jul;155(1):443–446. doi: 10.1128/jb.155.1.443-446.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moro I., Russell M. W. Ultrastructural localization of protein antigens I/II and III in Streptococcus mutans. Infect Immun. 1983 Jul;41(1):410–413. doi: 10.1128/iai.41.1.410-413.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Morrison M. Lactoperoxidase-catalyzed iodination as a tool for investigation of proteins. Methods Enzymol. 1980;70(A):214–220. doi: 10.1016/s0076-6879(80)70051-4. [DOI] [PubMed] [Google Scholar]
  25. Mouton C., Reynolds H. S., Genco R. J. Characterization of tufted streptococci isolated from the "corn cob" configuration of human dental plaque. Infect Immun. 1980 Jan;27(1):235–245. doi: 10.1128/iai.27.1.235-245.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Nesbitt W. E., Staat R. H., Rosan B., Taylor K. G., Doyle R. J. Association of protein with the cell wall of Streptococcus mutans. Infect Immun. 1980 Apr;28(1):118–126. doi: 10.1128/iai.28.1.118-126.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Overbeeke N., Van Scharrenburg G., Lugtenberg B. Antigenic relationships between pore proteins of Escherichia coli K12. Eur J Biochem. 1980 Sep;110(1):247–254. doi: 10.1111/j.1432-1033.1980.tb04862.x. [DOI] [PubMed] [Google Scholar]
  28. Ranhand J. M. Inhibition of the development of competence in Streptococcus sanguis (Wicky) by reagents that interact with sulfhydryl groups: discernment of the competence process. J Bacteriol. 1974 Jun;118(3):1041–1050. doi: 10.1128/jb.118.3.1041-1050.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Reusch V. M., Jr Isolation and analysis of sacculi from Streptococcus sanguis. J Bacteriol. 1982 Sep;151(3):1543–1552. doi: 10.1128/jb.151.3.1543-1552.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rosan B. Antigens of Streptococcus sanguis. Infect Immun. 1973 Feb;7(2):205–211. doi: 10.1128/iai.7.2.205-211.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rosan B., Malamud D., Appelbaum B., Golub E. Characteristic differences between saliva-dependent aggregation and adhesion of streptococci. Infect Immun. 1982 Jan;35(1):86–90. doi: 10.1128/iai.35.1.86-90.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Russell R. R. Comparison of oral Streptococcus mutans AHT with strains of serotypes a and g by biochemical and electrophoretic methods. Arch Oral Biol. 1979;24(8):617–619. doi: 10.1016/0003-9969(79)90023-2. [DOI] [PubMed] [Google Scholar]
  33. Russell R. R. Distribution of cross-reactive antigens A and B in Streptococcus mutans and other oral streptococci. J Gen Microbiol. 1980 Jun;118(2):383–388. doi: 10.1099/00221287-118-2-383. [DOI] [PubMed] [Google Scholar]
  34. Schöller M., Klein J. P., Frank R. M. Common antigens of streptococcal and non-streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall-associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius, and Actinomyces viscosus. Infect Immun. 1981 Jan;31(1):52–60. doi: 10.1128/iai.31.1.52-60.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Siegel J. L., Hurst S. F., Liberman E. S., Coleman S. E., Bleiweis A. S. Mutanolysin-induced spheroplasts of Streptococcus mutants are true protoplasts. Infect Immun. 1981 Feb;31(2):808–815. doi: 10.1128/iai.31.2.808-815.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Simonson L. G., Shklair I. L. Gel electrophoresis of intracellular components of some cariogenic streptococci. J Dent Res. 1972 Mar-Apr;51(2):488–491. doi: 10.1177/00220345720510024001. [DOI] [PubMed] [Google Scholar]
  37. Swanson J. Surface-exposed protein antigens of the gonococcal outer membrane. Infect Immun. 1981 Dec;34(3):804–816. doi: 10.1128/iai.34.3.804-816.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Swanstrom R., Shank P. R. X-Ray Intensifying Screens Greatly Enhance the Detection by Autoradiography of the Radioactive Isotopes 32P and 125I. Anal Biochem. 1978 May;86(1):184–192. doi: 10.1016/0003-2697(78)90333-0. [DOI] [PubMed] [Google Scholar]
  39. Terleckyj B., Willett N. P., Shockman G. D. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun. 1975 Apr;11(4):649–655. doi: 10.1128/iai.11.4.649-655.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Zanders E. D., Lehner T. Separation and characterization of a protein antigen from cells of Streptococcus mutans. J Gen Microbiol. 1981 Feb;122(2):217–225. doi: 10.1099/00221287-122-2-217. [DOI] [PubMed] [Google Scholar]
  41. de Cueninck B. J. Surface protein of a Streptococcus agalactiae isolate. Infect Immun. 1979 Jun;24(3):637–641. doi: 10.1128/iai.24.3.637-641.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES