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. 1985 Jan;47(1):80–83. doi: 10.1128/iai.47.1.80-83.1985

Multiple forms of genes in pyelonephritogenic Escherichia coli encoding adhesins binding globoseries glycolipid receptors.

S Hull, S Clegg, C Sanborg Eden, R Hull
PMCID: PMC261471  PMID: 2856916

Abstract

Earlier studies have shown that the majority of Escherichia coli isolates from urinary tract infections which possess a D-mannose-resistant adhesin contain gene sequences homologous to the pap pilus gene sequences encoded on the recombinant plasmid pRHU845. In this report, several E. coli isolates from urinary tract infections were examined to determined the arrangement of pap-related sequences. Copies of gene sequences homologous to the entire pap operon were found to exist at multiple sites in the chromosomes of some strains. The position of the pap operon(s) with respect to adjacent sequences was also found to be variable.

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Selected References

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  1. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. doi: 10.1093/infdis/143.3.325. [DOI] [PubMed] [Google Scholar]
  2. Båga M., Normark S., Hardy J., O'Hanley P., Lark D., Olsson O., Schoolnik G., Falkow S. Nucleotide sequence of the papA gene encoding the Pap pilus subunit of human uropathogenic Escherichia coli. J Bacteriol. 1984 Jan;157(1):330–333. doi: 10.1128/jb.157.1.330-333.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clegg S. Cloning of genes determining the production of mannose-resistant fimbriae in a uropathogenic strain of Escherichia coli belonging to serogroup O6. Infect Immun. 1982 Nov;38(2):739–744. doi: 10.1128/iai.38.2.739-744.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clegg S., Pierce J. K. Organization of genes responsible for the production of mannose-resistant fimbriae of a uropathogenic Escherichia coli isolate. Infect Immun. 1983 Dec;42(3):900–906. doi: 10.1128/iai.42.3.900-906.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edén C. S., Freter R., Hagberg L., Hull R., Hull S., Leffler H., Schoolnik G. Inhibition of experimental ascending urinary tract infection by an epithelial cell-surface receptor analogue. Nature. 1982 Aug 5;298(5874):560–562. doi: 10.1038/298560a0. [DOI] [PubMed] [Google Scholar]
  6. Edén C. S., Hansson H. A. Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect Immun. 1978 Jul;21(1):229–237. doi: 10.1128/iai.21.1.229-237.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hull R. A., Gill R. E., Hsu P., Minshew B. H., Falkow S. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun. 1981 Sep;33(3):933–938. doi: 10.1128/iai.33.3.933-938.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hull R. A., Hull S. I., Falkow S. Frequency of gene sequences necessary for pyelonephritis-associated pili expression among isolates of Enterobacteriaceae from human extraintestinal infections. Infect Immun. 1984 Mar;43(3):1064–1067. doi: 10.1128/iai.43.3.1064-1067.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hull S. I., Hull R. A., Minshew B. H., Falkow S. Genetics of hemolysin of Escherichia coli. J Bacteriol. 1982 Aug;151(2):1006–1012. doi: 10.1128/jb.151.2.1006-1012.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones G. W., Isaacson R. E. Proteinaceous bacterial adhesins and their receptors. Crit Rev Microbiol. 1983;10(3):229–260. doi: 10.3109/10408418209113564. [DOI] [PubMed] [Google Scholar]
  11. Korhonen T. K., Nurmiaho E. L., Ranta H., Edén C. S. New Method for isolation of immunologically pure pili from Escherichia coli. Infect Immun. 1980 Feb;27(2):569–575. doi: 10.1128/iai.27.2.569-575.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Low D., David V., Lark D., Schoolnik G., Falkow S. Gene clusters governing the production of hemolysin and mannose-resistant hemagglutination are closely linked in Escherichia coli serotype O4 and O6 isolates from urinary tract infections. Infect Immun. 1984 Jan;43(1):353–358. doi: 10.1128/iai.43.1.353-358.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Normark S., Lark D., Hull R., Norgren M., Båga M., O'Hanley P., Schoolnik G., Falkow S. Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain. Infect Immun. 1983 Sep;41(3):942–949. doi: 10.1128/iai.41.3.942-949.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ofek I., Beachey E. H. Bacterial adherence. Adv Intern Med. 1980;25:503–532. [PubMed] [Google Scholar]
  16. Orskov I., Orskov F. Serology of Escherichia coli fimbriae. Prog Allergy. 1983;33:80–105. [PubMed] [Google Scholar]
  17. Svanborg Edén C., Gotschlich E. C., Korhonen T. K., Leffler H., Schoolnik G. Aspects on structure and function of pili on uropathogenic Escherichia coli. Prog Allergy. 1983;33:189–202. doi: 10.1159/000318330. [DOI] [PubMed] [Google Scholar]
  18. Svanborg Edén C., Hagberg L., Hanson L. A., Hull S., Hull R., Jodal U., Leffler H., Lomberg H., Straube E. Bacterial adherence--a pathogenetic mechanism in urinary tract infections caused by Escherichia coli. Prog Allergy. 1983;33:175–188. [PubMed] [Google Scholar]
  19. Svanborg Edén C., Hull R., Falkow S., Leffler H. Target cell specificity of wild-type E. coli and mutants and clones with genetically defined adhesins. Prog Food Nutr Sci. 1983;7(3-4):75–89. [PubMed] [Google Scholar]
  20. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Mäkelä P. H., Svanborg-Edén C., Källenius G., Svenson S. B., Hultberg H., Korhonen T. Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet. 1981 Dec 19;2(8260-61):1366–1369. doi: 10.1016/s0140-6736(81)92796-3. [DOI] [PubMed] [Google Scholar]

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