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. 1985 Jan;47(1):169–175. doi: 10.1128/iai.47.1.169-175.1985

Defective neutrophil and monocyte motility in patients with early onset periodontitis.

R C Page, T J Sims, F Geissler, L C Altman, D A Baab
PMCID: PMC261493  PMID: 3965394

Abstract

Several studies have documented suppressed polymorphonuclear neutrophil (PMN) chemotaxis in most patients with juvenile periodontitis. In contrast, data regarding PMN chemotaxis in patients with rapidly progressive periodontitis are very limited, and monocyte (MN) chemotaxis and random migration of PMNs or MNs from these patients have not been studied previously. Accordingly, we examined cell motility of PMNs and MNs from 27 patients with rapidly progressive periodontitis, 5 patients with juvenile periodontitis, and 37 normal control subjects by using a microchamber technique and the synthetic peptide N-formylmethionyl-leucyl-phenylalanine (FMLP) as the chemoattractant. As a group, PMNs and MNs from patients with rapidly progressive periodontitis manifested significantly enhanced random migration relative to control cells (P less than 0.001), suppressed directed migration (chemotaxis) at FMLP doses of 10(-9) and 10(-8) M (P less than 0.05), and enhanced directed migration at a dose of 10(-6) M FMLP (P less than 0.01). In contrast, PMNs from patients with juvenile periodontitis exhibited normal random migration, and directed migration was significantly suppressed at all doses of FMLP tested (P less than 0.05). An abnormality of either PMN or MN motility was observed in 26 of 27 patients with rapidly progressive periodontitis. Enhanced random migration was seen in PMNs in 63%, MNs in 39%, and both cell types in 26% of the patients. Suppressed chemotaxis was seen in PMNs in 85%, in MNs in 74%, and in both cell types in 69% of the patients. The prevalence and magnitude of abnormalities in motility were somewhat lower in treated than in untreated patients. Thus, most, if not all, of this subgroup of patients with early onset, highly destructive periodontitis have abnormalities in PMN or MN motility, and these defects may differ from those seen in cells from patients with the juvenile form of the disease.

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Selected References

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  1. Altman L. C., Page R. C., Ebersole J. L., Vandesteen E. G. Assessment of host defenses and serum antibodies to suspected periodontal pathogens in patients with various types of periodontitis. J Periodontal Res. 1982 Sep;17(5):495–497. doi: 10.1111/j.1600-0765.1982.tb02037.x. [DOI] [PubMed] [Google Scholar]
  2. Bowen T. J., Ochs H. D., Altman L. C., Price T. H., Van Epps D. E., Brautigan D. L., Rosin R. E., Perkins W. D., Babior B. M., Klebanoff S. J. Severe recurrent bacterial infections associated with defective adherence and chemotaxis in two patients with neutrophils deficient in a cell-associated glycoprotein. J Pediatr. 1982 Dec;101(6):932–940. doi: 10.1016/s0022-3476(82)80013-9. [DOI] [PubMed] [Google Scholar]
  3. Cainciola L. J., Genco R. J., Patters M. R., McKenna J., van Oss C. J. Defective polymorphonuclear leukocyte function in a human periodontal disease. Nature. 1977 Feb 3;265(5593):445–447. doi: 10.1038/265445a0. [DOI] [PubMed] [Google Scholar]
  4. Clark R. A., Kimball H. R. Defective granulocyte chemotaxis in the Chediak-Higashi syndrome. J Clin Invest. 1971 Dec;50(12):2645–2652. doi: 10.1172/JCI106765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clark R. A., Page R. C., Wilde G. Defective neutrophil chemotaxis in juvenile periodontitis. Infect Immun. 1977 Dec;18(3):694–700. doi: 10.1128/iai.18.3.694-700.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Genco R. J., Van Dyke T. E., Park B., Ciminelli M., Horoszewicz H. Neutrophil chemotaxis impairment in juvenile periodontitis: evaluation of specificity, adherence, deformability, and serum factors. J Reticuloendothel Soc. 1980 Dec;28(Suppl):81s–91s. [PubMed] [Google Scholar]
  7. LOE H., SILNESS J. PERIODONTAL DISEASE IN PREGNANCY. I. PREVALENCE AND SEVERITY. Acta Odontol Scand. 1963 Dec;21:533–551. doi: 10.3109/00016356309011240. [DOI] [PubMed] [Google Scholar]
  8. Lavine W. S., Maderazo E. G., Stolman J., Ward P. A., Cogen R. B., Greenblatt I., Robertson P. B. Impaired neutrophil chemotaxis in patients with juvenile and rapidly progressing periodontitis. J Periodontal Res. 1979 Jan;14(1):10–19. doi: 10.1111/j.1600-0765.1979.tb00213.x. [DOI] [PubMed] [Google Scholar]
  9. McArthur W. P., Tsai C. C., Baehni P. C., Genco R. J., Taichman N. S. Leukotoxic effects of Actinobacillus actinomycetemcomitans. Modulation by serum components. J Periodontal Res. 1981 Mar;16(2):159–170. doi: 10.1111/j.1600-0765.1981.tb00962.x. [DOI] [PubMed] [Google Scholar]
  10. Page R. C., Altman L. C., Ebersole J. L., Vandesteen G. E., Dahlberg W. H., Williams B. L., Osterberg S. K. Rapidly progressive periodontitis. A distinct clinical condition. J Periodontol. 1983 Apr;54(4):197–209. doi: 10.1902/jop.1983.54.4.197. [DOI] [PubMed] [Google Scholar]
  11. Page R. C., Bowen T., Altman L., Vandesteen E., Ochs H., Mackenzie P., Osterberg S., Engel L. D., Williams B. L. Prepubertal periodontitis. I. Definition of a clinical disease entity. J Periodontol. 1983 May;54(5):257–271. doi: 10.1902/jop.1983.54.5.257. [DOI] [PubMed] [Google Scholar]
  12. Page R. C., Schroeder H. E. Current status of the host response in chronic marginal periodontitis. J Periodontol. 1981 Sep;52(9):477–491. doi: 10.1902/jop.1981.52.9.477. [DOI] [PubMed] [Google Scholar]
  13. SILNESS J., LOE H. PERIODONTAL DISEASE IN PREGNANCY. II. CORRELATION BETWEEN ORAL HYGIENE AND PERIODONTAL CONDTION. Acta Odontol Scand. 1964 Feb;22:121–135. doi: 10.3109/00016356408993968. [DOI] [PubMed] [Google Scholar]
  14. Shurin S. B., Socransky S. S., Sweeney E., Stossel T. P. A neutrophil disorder induced by capnocytophaga, a dental micro-organism. N Engl J Med. 1979 Oct 18;301(16):849–854. doi: 10.1056/NEJM197910183011601. [DOI] [PubMed] [Google Scholar]
  15. Van Dyke T. E., Bartholomew E., Genco R. J., Slots J., Levine M. J. Inhibition of neutrophil chemotaxis by soluble bacterial products. J Periodontol. 1982 Aug;53(8):502–508. doi: 10.1902/jop.1982.53.8.502. [DOI] [PubMed] [Google Scholar]
  16. Van Dyke T. E., Horoszewicz H. U., Cianciola L. J., Genco R. J. Neutrophil chemotaxis dysfunction in human periodontitis. Infect Immun. 1980 Jan;27(1):124–132. doi: 10.1128/iai.27.1.124-132.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Dyke T. E., Horoszewicz H. U., Genco R. J. The polymorphonuclear leukocyte (PMNL) locomotor defect in juvenile periodontitis. Study of random migration, chemokinesis and chemotaxis. J Periodontol. 1982 Nov;53(11):682–687. doi: 10.1902/jop.1982.53.11.682. [DOI] [PubMed] [Google Scholar]
  18. Van Dyke T. E., Levine M. J., Tabak L. A., Genco R. J. Reduced chemotactic peptide binding in juvenile periodontitis: a model for neutrophil function. Biochem Biophys Res Commun. 1981 Jun 16;100(3):1278–1284. doi: 10.1016/0006-291x(81)91962-8. [DOI] [PubMed] [Google Scholar]
  19. Vandesteen G. E., Altman L. C., Page R. C. Peripheral blood leukocyte abnormalities and periodontal disease. A family study. J Periodontol. 1981 Apr;52(4):174–180. doi: 10.1902/jop.1981.52.4.174. [DOI] [PubMed] [Google Scholar]

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