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. 1985 Jan;47(1):294–300. doi: 10.1128/iai.47.1.294-300.1985

Enhancement of experimental bacteremia and endocarditis caused by dysgonic fermenter (DF-2) bacterium after treatment with methylprednisolone and after splenectomy.

T Butler, K H Johnston, Y Gutierrez, M Aikawa, R Cardaman
PMCID: PMC261511  PMID: 3965402

Abstract

The dysgonic fermenter-2 bacterium is a newly recognized fastidious gram-negative bacillus that causes bacteremia and sometimes endocarditis in immunocompromised persons after they are bitten by dogs. To develop an experimental model of this infection, we placed polyethylene catheters across the aortic valves of New Zealand white rabbits, which were inoculated intravenously the next day with dysgonic fermenter-2 bacteria. After 1 week, the rabbits were killed and the endocardial vegetations were homogenized for quantitative culture. Large inocula (1.3 X 10(10) to 2.1 X 10(10) viable bacteria) were required to produce infected vegetations. All infected rabbits had negative blood cultures at the time of autopsy and most developed serum agglutinins against dysgonic fermenter-2 bacteria. Three daily injections of methylprednisolone (30 mg/kg), starting the day before inoculation, significantly increased the incidence of endocarditis and the number of bacteria per gram of infected vegetation (P less than 0.05). Treatment with methylprednisolone prolonged the initial bacteremia and caused significant increases in the numbers of bacteria per gram of blood, spleen, and liver compared with those of untreated controls (P less than 0.05). Rabbits that had previously undergone splenectomy showed prolongation of the initial bacteremia but no significant increase in the incidence of infected vegetations. These results showed that the dysgonic fermenter-2 bacterium is a pathogen that causes endocarditis in rabbits but that it requires a large inoculum and produces blood culture-negative infections. Treatment with methylprednisolone enhances infection by prolonging the initial bacteremia and probably by diminishing bactericidal activity in the vegetations.

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Selected References

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  1. Archer G. L., Johnston J. L. Effect of type-specific active immunization on the development and progression of experimental Pseudomonas aeruginosa endocarditis. Infect Immun. 1979 Apr;24(1):167–173. doi: 10.1128/iai.24.1.167-173.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Badger S. J., Butler T., Kim C. K., Johnston K. H. Experimental Eikenella corrodens endocarditis in rabbits. Infect Immun. 1979 Mar;23(3):751–757. doi: 10.1128/iai.23.3.751-757.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bailie W. E., Stowe E. C., Schmitt A. M. Aerobic bacterial flora of oral and nasal fluids of canines with reference to bacteria associated with bites. J Clin Microbiol. 1978 Feb;7(2):223–231. doi: 10.1128/jcm.7.2.223-231.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown E. J., Hosea S. W., Frank M. M. The role of the spleen in experimental pneumococcal bacteremia. J Clin Invest. 1981 Apr;67(4):975–982. doi: 10.1172/JCI110148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chaudhuri A. K., Hartley R. B., Maddocks A. C. Waterhouse-Friderichsen syndrome caused by a DF-2 bacterium in a splenectomised patient. J Clin Pathol. 1981 Feb;34(2):172–173. doi: 10.1136/jcp.34.2.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Durack D. T., Beeson P. B., Petersdorf R. G. Experimental bacterial endocarditis. 3. Production and progress of the disease in rabbits. Br J Exp Pathol. 1973 Apr;54(2):142–151. [PMC free article] [PubMed] [Google Scholar]
  7. Durack D. T., Beeson P. B. Protective role of complement in experimental Escherichia coli endocarditis. Infect Immun. 1977 Apr;16(1):213–217. doi: 10.1128/iai.16.1.213-217.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Durack D. T. Experimental bacterial endocarditis. IV. Structure and evolution of very early lesions. J Pathol. 1975 Feb;115(2):81–89. doi: 10.1002/path.1711150204. [DOI] [PubMed] [Google Scholar]
  9. Findling J. W., Pohlmann G. P., Rose H. D. Fulminant gram-negative bacillemia (DF-2) following a dog bite in an asplenic woman. Am J Med. 1980 Jan;68(1):154–156. doi: 10.1016/0002-9343(80)90188-6. [DOI] [PubMed] [Google Scholar]
  10. Francioli P. B., Freedman L. R. Streptococcal infection of endocardial and other intravascular vegetations in rabbits: natural history and effect of dexamethasone. Infect Immun. 1979 May;24(2):483–491. doi: 10.1128/iai.24.2.483-491.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Garrison P. K., Freedman L. R. Experimental endocarditis I. Staphylococcal endocarditis in rabbits resulting from placement of a polyethylene catheter in the right side of the heart. Yale J Biol Med. 1970 Jun;42(6):394–410. [PMC free article] [PubMed] [Google Scholar]
  12. Golshan N., Barker C. F., MacGregor R. R. Granulocyte function in recipients of renal transplant from live related donors and from cadavers. J Infect Dis. 1980 Dec;142(6):876–883. doi: 10.1093/infdis/142.6.876. [DOI] [PubMed] [Google Scholar]
  13. Hinrichs J. H., Dunkelberg W. E. DF-2 septicemia after splenectomy: epidemiology and immunologic response. South Med J. 1980 Dec;73(12):1638–1640. doi: 10.1097/00007611-198012000-00030. [DOI] [PubMed] [Google Scholar]
  14. Kaspar R. L., Drutz D. J. Perihepatitis and hepatitis as complications of experimental endocarditis due to Neisseria gonorrhoeae in the rabbit. J Infect Dis. 1977 Jul;136(1):37–42. doi: 10.1093/infdis/136.1.37. [DOI] [PubMed] [Google Scholar]
  15. MARTIN S. P., KERBY G. P., HOLLAND B. C. The effect of thorotrast on the removal of bacteria in the splanchnic area of the intact animal. J Immunol. 1952 Mar;68(3):293–296. [PubMed] [Google Scholar]
  16. Manganiello A. D., Socransky S. S., Smith C., Propas D., Oram V., Dogon I. L. Attempts to increase viable count recovery of human supragingival dental plaque. J Periodontal Res. 1977 Mar;12(2):107–119. doi: 10.1111/j.1600-0765.1977.tb00113.x. [DOI] [PubMed] [Google Scholar]
  17. Martone W. J., Zuehl R. W., Minson G. E., Scheld W. M. Postsplenectomy sepsis with DF-2: report of a case with isolation of the organism from the patient's dog. Ann Intern Med. 1980 Sep;93(3):457–458. doi: 10.7326/0003-4819-93-3-457. [DOI] [PubMed] [Google Scholar]
  18. Meddens M. J., Thompson J., Bauer W. C., van Furth R. Role of granulocytes and monocytes in experimental Escherichia coli endocarditis. Infect Immun. 1984 Feb;43(2):491–496. doi: 10.1128/iai.43.2.491-496.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Meddens M. J., Thompson J., Eulderink F., Bauer W. C., Mattie H., van Furth R. Role of granulocytes in experimental Streptococcus sanguis endocarditis. Infect Immun. 1982 Apr;36(1):325–332. doi: 10.1128/iai.36.1.325-332.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moxon E. R., Goldthorn J. F., Schwartz A. D. Haemophilus influenzae b infection in rats: effect of splenectomy on bloodstream and meningeal invasion after intravenous and intranasal inoculations. Infect Immun. 1980 Mar;27(3):872–875. doi: 10.1128/iai.27.3.872-875.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ofori-Adjei D., Blackledge P., O'Neill P. Meningitis caused by dysgonic fermenter type 2 (DF 2) organism in a previously healthy adult. Br Med J (Clin Res Ed) 1982 Jul 24;285(6337):263–264. doi: 10.1136/bmj.285.6337.263-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sande M. A., Johnson M. L. Antimicrobial therapy of experimental endocarditis caused by Staphylococcus aureus. J Infect Dis. 1975 Apr;131(4):367–375. doi: 10.1093/infdis/131.4.367. [DOI] [PubMed] [Google Scholar]
  23. Schlossberg D. Septicemia caused by DF-2. J Clin Microbiol. 1979 Feb;9(2):297–298. doi: 10.1128/jcm.9.2.297-298.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schoen R. T., Wohlgelernter D., Barden G. E., Swartz T. J. Infection with CDC group DF-2 gram-negative rod: report of two cases. Arch Intern Med. 1980 May;140(5):657–658. [PubMed] [Google Scholar]
  25. Shankar P. S., Scott J. H., Anderson C. L. Atypical endocarditis due to gram-negative bacillus transmitted by dog bite. South Med J. 1980 Dec;73(12):1640–1641. doi: 10.1097/00007611-198012000-00031. [DOI] [PubMed] [Google Scholar]
  26. Whitaker A. N. The effect of previous splenectomy on the course of pneumococcal bacteriaemia in mice. J Pathol Bacteriol. 1968 Apr;95(2):357–376. doi: 10.1002/path.1700950203. [DOI] [PubMed] [Google Scholar]
  27. Yersin B. R., Glauser M. P., Freedman L. R. Effect of nitrogen mustard on natural history of right-sided streptococcal endocarditis in rabbits: role for cellular host defenses. Infect Immun. 1982 Jan;35(1):320–325. doi: 10.1128/iai.35.1.320-325.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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