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. 1985 Jan;47(1):338–340. doi: 10.1128/iai.47.1.338-340.1985

Enterotoxin production, presence of colonization factor antigen I, and adherence to HeLa cells by Escherichia coli O128 strains belonging to different O subgroups.

B E Guth, L M Silva, I C Scaletsky, M R Toledo, L R Trabulsi
PMCID: PMC261521  PMID: 3880728

Abstract

Strains of three subgroups of Escherichia coli O128 were studied. Enterotoxin production was observed in 30 (91%) O128ac strains, whereas strains of subgroups O128ab and O128ad were not toxigenic. CFA/I was only found in two serotypes of subgroup O128ac, all of them producing heat-stable enterotoxin except for one which produced both toxins. None of the strains studied produced CFA/II. In a binding test with HeLa cells, localized adherence was found only in strains of subgroup O128ab; diffuse adherence occurred in strains of subgroup O128ac. As flagellar antigens were specific in subgroups ab and ac and toxin production was observed only in subgroup ac, the present results suggest that subgroup and serotype are useful markers for O128 strains that are enterotoxigenic or enteropathogenic.

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Selected References

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  1. Cravioto A., Scotland S. M., Rowe B. Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans. Infect Immun. 1982 Apr;36(1):189–197. doi: 10.1128/iai.36.1.189-197.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darfeuille A., Lafeuille B., Joly B., Cluzel R. A new colonization factor antigen (CFA/III) produced by enteropathogenic Escherichia coli O128:B12. Ann Microbiol (Paris) 1983 Jan-Feb;134A(1):53–64. doi: 10.1016/0769-2609(83)90103-5. [DOI] [PubMed] [Google Scholar]
  3. DeBoy J. M., 2nd, Wachsmuth I. K., Davis B. R. Serotypes of enterotoxigenic Escherichia coli isolated in the United States. Infect Immun. 1980 Aug;29(2):361–368. doi: 10.1128/iai.29.2.361-368.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  5. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  6. Evans D. G., Evans D. J., Jr New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infect Immun. 1978 Aug;21(2):638–647. doi: 10.1128/iai.21.2.638-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans D. G., Evans D. J., Jr, Pierce N. F. Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli. Infect Immun. 1973 Jun;7(6):873–880. doi: 10.1128/iai.7.6.873-880.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gurwith M., Hinde D., Gross R., Rowe B. A prospective study of enteropathogenic Escherichia coli in endemic diarrheal disease. J Infect Dis. 1978 Mar;137(3):292–297. doi: 10.1093/infdis/137.3.292. [DOI] [PubMed] [Google Scholar]
  9. Levine M. M., Bergquist E. J., Nalin D. R., Waterman D. H., Hornick R. B., Young C. R., Sotman S. Escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet. 1978 May 27;1(8074):1119–1122. doi: 10.1016/s0140-6736(78)90299-4. [DOI] [PubMed] [Google Scholar]
  10. Levine M. M., Ristaino P., Sack R. B., Kaper J. B., Orskov F., Orskov I. Colonization factor antigens I and II and type 1 somatic pili in enterotoxigenic Escherichia coli: relation to enterotoxin type. Infect Immun. 1983 Feb;39(2):889–897. doi: 10.1128/iai.39.2.889-897.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Merson M. H., Rowe B., Black R. E., Huq I., Gross R. J., Eusof A. Use of antisera for identification of enterotoxigenic Escherichia coli. Lancet. 1980 Aug 2;2(8188):222–224. doi: 10.1016/s0140-6736(80)90118-x. [DOI] [PubMed] [Google Scholar]
  12. Orskov F., Orskov I., Evans D. J., Jr, Sack R. B., Sack D. A., Wadström T. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med Microbiol Immunol. 1976 Jun 1;162(2):73–80. doi: 10.1007/BF02121318. [DOI] [PubMed] [Google Scholar]
  13. Orskov I., Orskov F., Jann B., Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977 Sep;41(3):667–710. doi: 10.1128/br.41.3.667-710.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reis M. H., Castro A. F., Toledo M. R., Trabulsi L. R. Production of heat-stable enterotoxin by the O128 serogroup of Escherichia coli. Infect Immun. 1979 Apr;24(1):289–290. doi: 10.1128/iai.24.1.289-290.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reis M. H., Guth B. E., Gomes T. A., Murahovschi J., Trabulsi L. R. Frequency of Escherichia coli strains producing heat-labile toxin or heat-stable toxin or both in children with and without diarrhea in São Paulo. J Clin Microbiol. 1982 Jun;15(6):1062–1064. doi: 10.1128/jcm.15.6.1062-1064.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reis M. H., Matos D. P., de Castro A. F., Toledo M. R., Trabulsi L. R. Relationship among enterotoxigenic phenotypes, serotypes, and sources of strains in enterotoxigenic Escherichia coli. Infect Immun. 1980 Apr;28(1):24–27. doi: 10.1128/iai.28.1.24-27.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sack D. A., Merson M. H., Wells J. G., Sack R. B., Morris G. K. Diarrhoea associated with heat-stable enterotoxin-producing strains of Escherichia coli. Lancet. 1975 Aug 9;2(7928):239–241. doi: 10.1016/s0140-6736(75)90958-7. [DOI] [PubMed] [Google Scholar]
  18. Sack R. B. Human diarrheal disease caused by enterotoxigenic Escherichia coli. Annu Rev Microbiol. 1975;29:333–353. doi: 10.1146/annurev.mi.29.100175.002001. [DOI] [PubMed] [Google Scholar]
  19. Scaletsky I. C., Silva M. L., Trabulsi L. R. Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun. 1984 Aug;45(2):534–536. doi: 10.1128/iai.45.2.534-536.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scotland S. M., Day N. P., Cravioto A., Thomas L. V., Rowe B. Production of heat-labile or heat-stable enterotoxins by strains of Escherichia coli belonging to serogroups O44, O114, and O128. Infect Immun. 1981 Jan;31(1):500–503. doi: 10.1128/iai.31.1.500-503.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TAYLOR J., CHARTER R. E. Escherichia coli 0.128 causing gastroenteritis of infants. J Clin Pathol. 1955 Nov;8(4):276–281. doi: 10.1136/jcp.8.4.276. [DOI] [PMC free article] [PubMed] [Google Scholar]

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