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. 1984 Nov;46(2):384–388. doi: 10.1128/iai.46.2.384-388.1984

Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3.

M Caroff, D R Bundle, M B Perry, J W Cherwonogrodzky, J R Duncan
PMCID: PMC261543  PMID: 6437981

Abstract

Antigenic phenol-phase soluble lipopolysaccharide isolated from Brucella abortus 1119-3 by hot phenol-water extraction was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, controlled hydrolysis, periodate oxidation, methylation, and 1H and 13C nuclear magnetic resonance studies to be an S-type lipopolysaccharide which could be cleaved to yield a lipid A and an O-chain polysaccharide identified as an unbranched linear homopolymer of 1,2-linked 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl residues. The serological reactivity of bovine antiserum to B. abortus 1119-3 with the lipopolysaccharides of Yersinia enterocolitica serotype O:9 and Vibrio cholerae species has now been related to the occurrence of 1,2-linked N-acylated 4-amino-4,6-dideoxy-alpha-D-mannopyranosyl units in the O-chain polysaccharides of their lipopolysaccharides.

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Selected References

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  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Caroff M., Bundle D. R., Perry M. B. Structure of the O-chain of the phenol-phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9. Eur J Biochem. 1984 Feb 15;139(1):195–200. doi: 10.1111/j.1432-1033.1984.tb07994.x. [DOI] [PubMed] [Google Scholar]
  3. Diaz R., Jones L. M., Leong D., Wilson J. B. Surface antigens of smooth brucellae. J Bacteriol. 1968 Oct;96(4):893–901. doi: 10.1128/jb.96.4.893-901.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diaz R., Levieux D. Rôle respiectif en sérologie de la brucellose bovine des antigènes et des immunoglobulines G 1 et G 2 dans les tests d'agglutination, de Coombs et au Rose Bengale ainsi que dans le phénomène de zone. C R Acad Sci Hebd Seances Acad Sci D. 1972 Mar 6;274(10):1593–1596. [PubMed] [Google Scholar]
  5. Gatt R., Berman E. R. A rapid procedure for the estimation of amino sugars on a micro scale. Anal Biochem. 1966 Apr;15(1):167–171. doi: 10.1016/0003-2697(66)90262-4. [DOI] [PubMed] [Google Scholar]
  6. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  7. Janda J., Work E. A colorimetric estimation of lipopolysaccharides. FEBS Lett. 1971 Sep 1;16(4):343–345. doi: 10.1016/0014-5793(71)80386-1. [DOI] [PubMed] [Google Scholar]
  8. Kenne L., Lindberg B., Unger P., Holme T., Holmgren J. Structural studies of the Vibrio cholerae O-antigen. Carbohydr Res. 1979 Jan;68(1):C14–C16. doi: 10.1016/s0008-6215(00)84073-2. [DOI] [PubMed] [Google Scholar]
  9. Lamb V. L., Jones L. M., Schurig G. G., Berman D. T. Enzyme-linked immunosorbent assay for bovine immunoglobulin subclass-specific response to Brucella abortus lipopolysaccharides. Infect Immun. 1979 Oct;26(1):240–247. doi: 10.1128/iai.26.1.240-247.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lindberg A. A., Haeggman S., Karlson K., Carlsson H. E., Mair N. S. Enzyme immunoassay of the antibody response to Brucella and Yersinia enterocolitica 09 infections in humans. J Hyg (Lond) 1982 Apr;88(2):295–307. doi: 10.1017/s0022172400070157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Magee J. T. An enzyme-labelled immunosorbent assay for Brucella abortus antibodies. J Med Microbiol. 1980 Feb;13(1):167–172. doi: 10.1099/00222615-13-1-167. [DOI] [PubMed] [Google Scholar]
  12. Moreno E., Berman D. T., Boettcher L. A. Biological activities of Brucella abortus lipopolysaccharides. Infect Immun. 1981 Jan;31(1):362–370. doi: 10.1128/iai.31.1.362-370.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moreno E., Pitt M. W., Jones L. M., Schurig G. G., Berman D. T. Purification and characterization of smooth and rough lipopolysaccharides from Brucella abortus. J Bacteriol. 1979 May;138(2):361–369. doi: 10.1128/jb.138.2.361-369.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perry M. B., Daoust V. Analysis of 2-amino-2,6-dideoxyhexoses by partition chromatography. Can J Biochem. 1973 Sep;51(9):1335–1339. doi: 10.1139/o73-176. [DOI] [PubMed] [Google Scholar]
  15. Redmond J. W. The structure of the O-antigenic side chain of the lipopolysaccharide of Vibrio cholerae 569B (Inaba). Biochim Biophys Acta. 1979 May 1;584(2):346–352. doi: 10.1016/0304-4165(79)90280-0. [DOI] [PubMed] [Google Scholar]
  16. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]

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