Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1984 Dec;46(3):854–856. doi: 10.1128/iai.46.3.854-856.1984

Identification and properties of distinct sucrose and glucose phosphotransferase enzyme II activities in Streptococcus mutans 6715g.

G R Jacobson, C S Mimura, P J Scott, P W Thompson
PMCID: PMC261626  PMID: 6500714

Abstract

We investigated phosphoenolpyruvate-dependent phosphotransferase system enzyme II activities for sucrose and glucose in Streptococcus mutans 6715g. Two integral membrane proteins, enzyme IIscr and enzyme IIglc, each specific for its sugar substrate, sucrose or glucose, were identified by their abilities to catalyze specific sugar:sugar-phosphate exchange reactions. Some of the properties of these two transport proteins are also presented.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bozzola J. J., Kuramitsu H. K., Maynard M. T. Localization of Streptococcus mutans GS-5 glucosyltransferases and intracellular invertase. Infect Immun. 1981 May;32(2):830–839. doi: 10.1128/iai.32.2.830-839.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown A. T., Wittenberger C. L. Mannitol and sorbitol catabolism in Streptococcus mutans. Arch Oral Biol. 1973 Jan;18(1):117–126. doi: 10.1016/0003-9969(73)90026-5. [DOI] [PubMed] [Google Scholar]
  3. Calmes R. Involvement of phosphoenolpyruvate in the catabolism of caries-conducive disaccharides by Streptococcus mutans: lactose transport. Infect Immun. 1978 Mar;19(3):934–942. doi: 10.1128/iai.19.3.934-942.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Erni B., Trachsel H., Postma P. W., Rosenbusch J. P. Bacterial phosphotransferase system. Solubilization and purification of the glucose-specific enzyme II from membranes of Salmonella typhimurium. J Biol Chem. 1982 Nov 25;257(22):13726–13730. [PubMed] [Google Scholar]
  5. Jacobson G. R., Lee C. A., Saier M. H., Jr Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system. J Biol Chem. 1979 Jan 25;254(2):249–252. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Liberman E. S., Bleiweis A. S. Glucose phosphoenolpyruvate-dependent phosphotransferase system of Streptococcus mutans GS5 studied by using cell-free extracts. Infect Immun. 1984 May;44(2):486–492. doi: 10.1128/iai.44.2.486-492.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maryanski J. H., Wittenberger C. L. Mannitol transport in Streptococcus mutans. J Bacteriol. 1975 Dec;124(3):1475–1481. doi: 10.1128/jb.124.3.1475-1481.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mimura C. S., Eisenberg L. B., Jacobson G. R. Resolution of the phosphotransferase enzymes of Streptococcus mutans: purification and preliminary characterization of a heat-stable phosphocarrier protein. Infect Immun. 1984 Jun;44(3):708–715. doi: 10.1128/iai.44.3.708-715.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Padan E., Zilberstein D., Schuldiner S. pH homeostasis in bacteria. Biochim Biophys Acta. 1981 Dec;650(2-3):151–166. doi: 10.1016/0304-4157(81)90004-6. [DOI] [PubMed] [Google Scholar]
  11. Saier M. H., Jr Bacterial phosphoenolpyruvate: sugar phosphotransferase systems: structural, functional, and evolutionary interrelationships. Bacteriol Rev. 1977 Dec;41(4):856–871. doi: 10.1128/br.41.4.856-871.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Saier M. H., Jr, Feucht B. U. Coordinate regulation of adenylate cyclase and carbohydrate permeases by the phosphoenolpyruvate:sugar phosphotransferase system in Salmonella typhimurium. J Biol Chem. 1975 Sep 10;250(17):7078–7080. [PubMed] [Google Scholar]
  13. Saier M. H., Jr, Feucht B. U., Mora W. K. Sugar phosphate: sugar transphosphorylation and exchange group translocation catalyzed by the enzyme 11 complexes of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1977 Dec 25;252(24):8899–8907. [PubMed] [Google Scholar]
  14. Schachtele C. F. Glucose transport in Streptococcus mutans: preparation of cytoplasmic membranes and characteristics of phosphotransferase activity. J Dent Res. 1975 Mar-Apr;54(2):330–338. [PubMed] [Google Scholar]
  15. Schachtele C. F., Mayo J. A. Phosphoenolpyruvate-dependent glucose transport in oral streptococci. J Dent Res. 1973 Nov-Dec;52(6):1209–1215. doi: 10.1177/00220345730520060801. [DOI] [PubMed] [Google Scholar]
  16. Schmid K., Schupfner M., Schmitt R. Plasmid-mediated uptake and metabolism of sucrose by Escherichia coli K-12. J Bacteriol. 1982 Jul;151(1):68–76. doi: 10.1128/jb.151.1.68-76.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simoni R. D., Nakazawa T., Hays J. B., Roseman S. Sugar transport. IV. Isolation and characterization of the lactose phosphotransferase system in Staphylococcus aureus. J Biol Chem. 1973 Feb 10;248(3):932–940. [PubMed] [Google Scholar]
  18. Simoni R. D., Roseman S., Saier M. H., Jr Sugar transport. Properties of mutant bacteria defective in proteins of the phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1976 Nov 10;251(21):6584–6597. [PubMed] [Google Scholar]
  19. Slee A. M., Tanzer J. M. Phosphoenolpyruvate-dependent sucrose phosphotransferase activity in Streptococcus mutans NCTC 10449. Infect Immun. 1979 Jun;24(3):821–828. doi: 10.1128/iai.24.3.821-828.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. St Martin E. J., Wittenberger C. L. Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans. Infect Immun. 1979 Jun;24(3):865–868. doi: 10.1128/iai.24.3.865-868.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vadeboncoeur C., Mayrand D., Trahan L. A comparative study of enzymes involved in glucose phosphorylation in oral streptococci. J Dent Res. 1982 Jan;61(1):60–65. doi: 10.1177/00220345820610011401. [DOI] [PubMed] [Google Scholar]
  22. Vadeboncoeur C. Structure and properties of the phosphoenolpyruvate: glucose phosphotransferase system of oral streptococci. Can J Microbiol. 1984 Apr;30(4):495–502. doi: 10.1139/m84-073. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES