Abstract
The effect of hypothermia on pig leukocyte migration in vitro and in vivo was studied. Neutrophil chemotaxis in vitro under agarose was significantly impaired at 29 degrees C (2.7 +/- 0.6 [mean +/- standard error]; 37 degrees C, 7.1 +/- 1.1). Leukocytes isolated from hypothermic pigs and tested at 37 degrees C migrated normally (7.8 +/- 0.6). Neutrophil and monocyte migration in vivo was markedly reduced at 29 degrees C. Reduced inflammatory responses may contribute to increased infections during hypothermia.
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- Biggar W. D., Bohn D., Kent G. Neutrophil circulation and release from bone marrow during hypothermia. Infect Immun. 1983 May;40(2):708–712. doi: 10.1128/iai.40.2.708-712.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EISEMAN B., MALETTE W. G., SUMMERS W. B., TONG J. L., WOTKYNS R. S. Prolonged hypothermia in experimental pneumococcal peritonitis. J Clin Invest. 1956 Sep;35(9):940–946. doi: 10.1172/JCI103353. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frewen T. C., Swedlow D. B., Watcha M., Raphaely R. C., Godinez R. I., Heiser M. S., Kettrick R. G., Bruce D. A. Outcome in severe Reye syndrome with early pentobarbital coma and hypothermia. J Pediatr. 1982 Apr;100(4):663–665. doi: 10.1016/s0022-3476(82)80782-8. [DOI] [PubMed] [Google Scholar]
- GOWEN G. F., FRIOU G. J. The influence of hypothermia on experimental bacteremia in dogs. Surgery. 1961 Dec;50:919–921. [PubMed] [Google Scholar]
- HELMSWORTH J. A., STILES W. J., ELSTUN W. Changes in blood cellular elements in dogs during hypothermia. Surgery. 1955 Nov;38(5):843–846. [PubMed] [Google Scholar]
- Issekutz A. C., Biggar W. D. Influence of serum-derived chemotactic factors and bacterial products on human neutrophil chemotaxis. Infect Immun. 1977 Jan;15(1):212–220. doi: 10.1128/iai.15.1.212-220.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Issekutz A. C., Movat H. Z. The in vivo quantitation and kinetics of rabbit neutrophil leukocyte accumulation in the skin in response to chemotactic agents and Escherichia coli. Lab Invest. 1980 Mar;42(3):310–317. [PubMed] [Google Scholar]
- Issekutz T. B., Issekutz A. C., Movat H. Z. The in vivo quantitation and kinetics of monocyte migration into acute inflammatory tissue. Am J Pathol. 1981 Apr;103(1):47–55. [PMC free article] [PubMed] [Google Scholar]
- Lewin S., Brettman L. R., Holzman R. S. Infections in hypothermic patients. Arch Intern Med. 1981 Jun;141(7):920–925. [PubMed] [Google Scholar]
- Nelson R. D., Quie P. G., Simmons R. L. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol. 1975 Dec;115(6):1650–1656. [PubMed] [Google Scholar]
- Orr W., Ward P. A. Quantitation of leukotaxis in agarose by three different methods. J Immunol Methods. 1978;20:95–107. doi: 10.1016/0022-1759(78)90248-x. [DOI] [PubMed] [Google Scholar]
- PAZ R. A., SPECTOR W. G. The mononuclear-cell response to injury. J Pathol Bacteriol. 1962 Jul;84:85–103. doi: 10.1002/path.1700840111. [DOI] [PubMed] [Google Scholar]
- Ryan G. B. The origin and sequence of the cells found in the acute inflammatory response. Aust J Exp Biol Med Sci. 1967 Apr;45(2):149–162. doi: 10.1038/icb.1967.12. [DOI] [PubMed] [Google Scholar]
- Sabiston B. H., Rose J. E. Effect of cold exposure on the metabolism of immunoglobulins in rabbits. J Immunol. 1976 Jan;116(1):106–111. [PubMed] [Google Scholar]
- VILLALOBOS T. J., ADELSON E., BARILA T. G. Hematologic changes in hypothermic dogs. Proc Soc Exp Biol Med. 1955 Jun;89(2):192–196. doi: 10.3181/00379727-89-21754. [DOI] [PubMed] [Google Scholar]
- Walker R. I., Willemze R. Neutrophil kinetics and the regulation of granulopoiesis. Rev Infect Dis. 1980 Mar-Apr;2(2):282–292. doi: 10.1093/clinids/2.2.282. [DOI] [PubMed] [Google Scholar]
- van Oss C. J., Absolom D. R., Moore L. L., Park B. H., Humbert J. R. Effect of temperature on the chemotaxis, phagocytic engulfment, digestion and O2 consumption of human polymorphonuclear leukocytes. J Reticuloendothel Soc. 1980 Jun;27(6):561–565. [PubMed] [Google Scholar]