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Journal of Nematology logoLink to Journal of Nematology
. 1983 Jul;15(3):444–449.

On the Methodology of Nematode Extraction from Field Samples: Density Flotation Techniques

David R Viglierchio, Tom T Yamashita
PMCID: PMC2618295  PMID: 19295831

Abstract

Density flotation has been frequently used for the extraction of nematodes from field samples. Density flotation curves for four nematode species and five solutes have been prepared. The curves confirm that flotation was governed by several factors: solute density, solute osmotic activity, and physiological properties of the nematode species. Nematode viability and function can be adversely affected by improper selection of solute for density extraction of nematodes; nevertheless, some nematode species can be enriched from mixtures by density and solute selection.

Keywords: osmosis, viability

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