Skip to main content
Journal of Nematology logoLink to Journal of Nematology
. 1988 Jan;20(1):70–78.

A Method for Staining Nematode Secretions and Structures

D Premachandran, N Von Mende, R S Hussey, M A McClure
PMCID: PMC2618786  PMID: 19290186

Abstract

Secretions from amphids, phasmids, and excretory system were stained by incubating nematodes in 0.1% coomassie brilliant blue G-250 in 40% aqueous methanol containing 10% acetic acid on slides with coverslips sealed with nail polish or Zut. Nematodes incubated in this staining solution usually produced copious amounts of secretions from their amphids and excretory pore. Phasmids also stained dark blue, enabling them to be easily observed. Other biological dyes stained these secretions or were useful for differentiating specific morphological features of nematodes.

Keywords: secretion, amphid, phasmid, excretory system, stain, coomassie brilliant blue, exudate

Full Text

The Full Text of this article is available as a PDF (1.7 MB).


Articles from Journal of Nematology are provided here courtesy of Society of Nematologists

RESOURCES