Isozyme Phenotypes for the Identification of Meloidogyne Species

Abstract

Extensive studies during the last 20 years have demonstrated that enzyme phenotypes, especially those of esterases, are species-specific for Meloidogyne and can be used as reliable taxonomic characters for identification of most major and several minor species of this genus. Recent progress in electrophoretic procedures and advanced computer technology have made available automated electrophoretic apparati that can process very thin polyacrylamide slab gels on which the phenotypes of two or more enzymes can be revealed from the protein extract of a single Meloidogyne female. Presently, such apparati facilitate objective species identification. They also are convenient for performing routine field surveys to determine the relative distribution of major Meloidogyne species, conducting population dynamics studies in the field and in microplots, and testing the purity of greenhouse cultures.

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