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. 1985 Nov;50(2):506–509. doi: 10.1128/iai.50.2.506-509.1985

In vitro adherence of Escherichia coli to endometrial epithelial cells of rats and influence of estradiol.

Y Nishikawa, T Baba
PMCID: PMC261983  PMID: 3902646

Abstract

The influence of ovarian hormones on the adhesion of Escherichia coli to endometrial epithelial cells was investigated in an in vitro system. Endometrial cells liberated by collagenase from rat uteri were used. Optimal test conditions were obtained when 5 X 10(8) E. coli bacteria were added to 10(5) epithelial cells and incubated for 60 min. The adhesion of the organisms was inhibited by the addition of either mannose or alpha-methyl-D-mannopyranoside. When epithelial cells collected from uteri of estradiol-treated rats were used, the number of E. coli adhering to the cells was markedly lower than that adhering to epithelial cells collected from control rats. These results suggest that E. coli adheres to endometrial epithelial cells with so-called type 1 pili and that estradiol alters the nature of the endometrial epithelium and prevents the adherence of the organisms to the cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. T. Hormonal cohtrol of uterine lumen fluid retention in the rat. Am J Physiol. 1968 Apr;214(4):764–771. doi: 10.1152/ajplegacy.1968.214.4.764. [DOI] [PubMed] [Google Scholar]
  2. Baker D. A., Plotkin S. A. Enhancement of vaginal infection in mice by herpes simplex virus type II with progesterone. Proc Soc Exp Biol Med. 1978 Jun;158(2):131–134. doi: 10.3181/00379727-158-40156. [DOI] [PubMed] [Google Scholar]
  3. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. doi: 10.1093/infdis/143.3.325. [DOI] [PubMed] [Google Scholar]
  4. Buxton L. E., Murdoch R. N. Metabolic properties of mouse uterine endometrial cells after isolated with collagenase. Aust J Biol Sci. 1982;35(3):277–286. [PubMed] [Google Scholar]
  5. Catterall R. D. Influence of gestogenic contraceptive pills on vaginal candidosis. Br J Vener Dis. 1971 Feb;47(1):45–47. doi: 10.1136/sti.47.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cavalieri S. J., Snyder I. S. Effect of Escherichia coli alpha-hemolysin on human peripheral leukocyte function in vitro. Infect Immun. 1982 Sep;37(3):966–974. doi: 10.1128/iai.37.3.966-974.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cavalieri S. J., Snyder I. S. Effect of Escherichia coli alpha-hemolysin on human peripheral leukocyte viability in vitro. Infect Immun. 1982 May;36(2):455–461. doi: 10.1128/iai.36.2.455-461.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Davis C. P., Avots-Avotins A. E., Fader R. C. Evidence for a bladder cell glycolipid receptor for Escherichia coli and the effect of neuraminic acid and colominic acid on adherence. Infect Immun. 1981 Dec;34(3):944–948. doi: 10.1128/iai.34.3.944-948.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Edén C. S., Hanson L. A., Jodal U., Lindberg U., Akerlund A. S. Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. Lancet. 1976 Sep 4;1(7984):490–492. [PubMed] [Google Scholar]
  10. Gibbons R. J. Bacterial adherence to mucosal surfaces and its inhibition by secretory antibodies. Adv Exp Med Biol. 1974;45(0):315–325. doi: 10.1007/978-1-4613-4550-3_38. [DOI] [PubMed] [Google Scholar]
  11. HAWK H. W., BRINSFIELD T. H., TURNER G. D., WHITMORE G. W., NORCROSS M. A. EFFECT OF OVARIAN STATUS ON INDUCED ACUTE INFLAMMATORY RESPONSES IN CATTLE UTERI. Am J Vet Res. 1964 Mar;25:362–366. [PubMed] [Google Scholar]
  12. HAWK H. W., TURNER G. D., SYKES J. F. The effect of ovarian hormones on the uterine defense mechanism during the early stages of induced infection. Am J Vet Res. 1960 Jul;21:644–648. [PubMed] [Google Scholar]
  13. HAWK H. W., TURNER G. D., SYKES J. F. Variation in the inflammatory response and bactericidal activity of the sheep uterus during the estrous cycle. Am J Vet Res. 1961 Jul;22:689–692. [PubMed] [Google Scholar]
  14. Hilton A. L., Richmond S. J., Milne J. D., Hindley F., Clarke S. K. Chlamydia A in the female genital tract. Br J Vener Dis. 1974 Feb;50(1):1–10. doi: 10.1136/sti.50.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kita E., Matsuura H., Kashiba S. A mouse model for the study of gonococcal genital infection. J Infect Dis. 1981 Jan;143(1):67–70. doi: 10.1093/infdis/143.1.67. [DOI] [PubMed] [Google Scholar]
  16. Nishikawa Y. Adherence of Escherichia coli in pathogenesis of endometritis and effects of estradiol examined by scanning electron microscopy. Infect Immun. 1985 Jan;47(1):318–321. doi: 10.1128/iai.47.1.318-321.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nishikawa Y., Baba T. Effects of ovarian hormones on manifestation of purulent endometritis in rat uteruses infected with Escherichia coli. Infect Immun. 1985 Jan;47(1):311–317. doi: 10.1128/iai.47.1.311-317.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nishikawa Y., Baba T., Imori T. Effect of the estrous cycle on uterine infection induced by Escherichia coli. Infect Immun. 1984 Feb;43(2):678–683. doi: 10.1128/iai.43.2.678-683.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oriel J. D., Partridge B. M., Denny M. J., Coleman J. C. Genital yeast infections. Br Med J. 1972 Dec 30;4(5843):761–764. doi: 10.1136/bmj.4.5843.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Parsons C. L., Greenspan C., Moore S. W., Mulholland S. G. Role of surface mucin in primary antibacterial defense of bladder. Urology. 1977 Jan;9(1):48–52. doi: 10.1016/0090-4295(77)90284-9. [DOI] [PubMed] [Google Scholar]
  21. Pauli B. U., Anderson S. N., Memoli V. A., Kuettner K. E. The isolation and characterization in vitro of normal epithelial cells, endothelial cells and fibroblasts from rat urinary bladder. Tissue Cell. 1980;12(3):419–436. doi: 10.1016/0040-8166(80)90033-6. [DOI] [PubMed] [Google Scholar]
  22. Ramphal R., Small P. M., Shands J. W., Jr, Fischlschweiger W., Small P. A., Jr Adherence of Pseudomonas aeruginosa to tracheal cells injured by influenza infection or by endotracheal intubation. Infect Immun. 1980 Feb;27(2):614–619. doi: 10.1128/iai.27.2.614-619.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rank R. G., White H. J., Hough A. J., Jr, Pasley J. N., Barron A. L. Effect of estradiol on chlamydial genital infection of female guinea pigs. Infect Immun. 1982 Nov;38(2):699–705. doi: 10.1128/iai.38.2.699-705.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Smith H. W., Huggins M. B. The association of the O18, K1 and H7 antigens and the Co1V plasmid of a strain of Escherichia coli with its virulence and immunogenicity. J Gen Microbiol. 1980 Dec;121(2):387–400. doi: 10.1099/00221287-121-2-387. [DOI] [PubMed] [Google Scholar]
  25. Svanborg-Edén C., Svennerholm A. M. Secretory immunoglobulin A and G antibodies prevent adhesion of Escherichia coli to human urinary tract epithelial cells. Infect Immun. 1978 Dec;22(3):790–797. doi: 10.1128/iai.22.3.790-797.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Verweij-van Vught A. M., van den Bosch J. F., Namavar F., Sparrius M., MacLaren D. M. K antigens of Escherichia coli and virulence in urinary-tract infection: studies in a mouse model. J Med Microbiol. 1983 May;16(2):147–155. doi: 10.1099/00222615-16-2-147. [DOI] [PubMed] [Google Scholar]
  27. Welch R. A., Dellinger E. P., Minshew B., Falkow S. Haemolysin contributes to virulence of extra-intestinal E. coli infections. Nature. 1981 Dec 17;294(5842):665–667. doi: 10.1038/294665a0. [DOI] [PubMed] [Google Scholar]
  28. Williams P. H. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. doi: 10.1128/iai.26.3.925-932.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wira C. R., Sandoe C. P. Hormonal regulation of immunoglobulins: influence of estradiol on immunoglobulins A and G in the rat uterus. Endocrinology. 1980 Mar;106(3):1020–1026. doi: 10.1210/endo-106-3-1020. [DOI] [PubMed] [Google Scholar]

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