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. 1985 Nov;50(2):595–597. doi: 10.1128/iai.50.2.595-597.1985

Neutralization of Chlamydia trachomatis cell culture infection by serovar-specific monoclonal antibodies.

M E Lucero, C C Kuo
PMCID: PMC262000  PMID: 4055037

Abstract

Seventeen monoclonal antibodies (MAs) were tested. Five of seven serovar-specific MAs from five different serovars and two of five subspecies-specific MAs showed neutralizing activity as well as serovar specificity. No species- or genus-specific MAs showed neutralizing activity. No neutralization occurred without complement. Results indicate neutralization was serovar specific and complement dependent.

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Selected References

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  1. Barnes R. C., Wang S. P., Kuo C. C., Stamm W. E. Rapid immunotyping of Chlamydia trachomatis with monoclonal antibodies in a solid-phase enzyme immunoassay. J Clin Microbiol. 1985 Oct;22(4):609–613. doi: 10.1128/jcm.22.4.609-613.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benjamin D. C., Berzofsky J. A., East I. J., Gurd F. R., Hannum C., Leach S. J., Margoliash E., Michael J. G., Miller A., Prager E. M. The antigenic structure of proteins: a reappraisal. Annu Rev Immunol. 1984;2:67–101. doi: 10.1146/annurev.iy.02.040184.000435. [DOI] [PubMed] [Google Scholar]
  3. Caldwell H. D., Perry L. J. Neutralization of Chlamydia trachomatis infectivity with antibodies to the major outer membrane protein. Infect Immun. 1982 Nov;38(2):745–754. doi: 10.1128/iai.38.2.745-754.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark R. B., Nachamkin I., Schatzki P. F., Dalton H. P. Localization of distinct surface antigens on Chlamydia trachomatis HAR-13 by immune electron microscopy with monoclonal antibodies. Infect Immun. 1982 Dec;38(3):1273–1278. doi: 10.1128/iai.38.3.1273-1278.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ey P. L., Russell-Jones G. J., Jenkin C. R. Isotypes of mouse IgG--I. Evidence for 'non-complement-fixing' IgG1 antibodies and characterization of their capacity to interfere with IgG2 sensitization of target red blood cells for lysis by complement. Mol Immunol. 1980 Jun;17(6):699–710. doi: 10.1016/0161-5890(80)90139-x. [DOI] [PubMed] [Google Scholar]
  6. FURNESS G., GRAHAM D. M., REEVE P. The titration of trachoma and inclusion blennorrhoea viruses in cell cultures. J Gen Microbiol. 1960 Dec;23:613–619. doi: 10.1099/00221287-23-3-613. [DOI] [PubMed] [Google Scholar]
  7. Howard L. V. Neutralization of Chlamydia trachomatis in cell culture. Infect Immun. 1975 Apr;11(4):698–703. doi: 10.1128/iai.11.4.698-703.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klaus G. G., Pepys M. B., Kitajima K., Askonas B. A. Activation of mouse complement by different classes of mouse antibody. Immunology. 1979 Dec;38(4):687–695. [PMC free article] [PubMed] [Google Scholar]
  9. Peeling R., Maclean I. W., Brunham R. C. In vitro neutralization of Chlamydia trachomatis with monoclonal antibody to an epitope on the major outer membrane protein. Infect Immun. 1984 Nov;46(2):484–488. doi: 10.1128/iai.46.2.484-488.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stephens R. S., Kuo C. C., Tam M. R. Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture. J Clin Microbiol. 1982 Jul;16(1):4–7. doi: 10.1128/jcm.16.1.4-7.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stephens R. S., Tam M. R., Kuo C. C., Nowinski R. C. Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization. J Immunol. 1982 Mar;128(3):1083–1089. [PubMed] [Google Scholar]
  12. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]
  13. Wang S. P., Kuo C. C., Barnes R. C., Stephens R. S., Grayston J. T. Immunotyping of Chlamydia trachomatis with monoclonal antibodies. J Infect Dis. 1985 Oct;152(4):791–800. doi: 10.1093/infdis/152.4.791. [DOI] [PubMed] [Google Scholar]

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