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. 1985 Aug;49(2):414–416. doi: 10.1128/iai.49.2.414-416.1985

Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.

M L Gilpin, R R Russell, P Morrissey
PMCID: PMC262032  PMID: 3160662

Abstract

Chromosomal DNA from Streptococcus mutans strain MFe28 (serotype h) was cloned in the bacteriophage vector lambda L47.1. Two classes of recombinants were found which expressed glucosyltransferase activity in phage plaques: (i) gtfS, which expressed a glucosyltransferase synthesizing a water-soluble, dextranase-sensitive glucan, and (ii) gtfI, which expressed a primer-dependent glucosyltransferase synthesizing an insoluble glucan.

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Selected References

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