Abstract
The production of fimbrial adhesins K99 and F41 by enterotoxigenic Escherichia coli has been measured in steady-state chemostat experiments at various specific growth rates (microseconds) and in a recycling fermentor across a range of mu values falling to less than 0.004 h-1. It has been demonstrated that the production of K99 and F41 fimbriae is correlated with mu both in aerobic and anaerobic chemostat experiments. A significant production of fimbriae was only detected at mu values higher than 0.2 h-1. This behavior was further examined by culturing the bacteria in a recycling fermentor with complete biomass retention. It could be shown that the production of K99 and F41 fimbriae only occurred during balanced growth, with a high biomass yield at mu values higher than 0.04 h-1 corresponding to mass doubling times (td) of less than 17 h. The production of both fimbriae halted during balanced growth with a lower biomass yield (at mu values between 0.012 and 0.04 h-1 corresponding to td values between 17 and 58 h) and unbalanced stringent growth (at mu values lower than 0.012 h-1 or td values higher than 58 h). The external pH of the medium greatly influenced the production of both K99 and F41 fimbriae. At pH values lower than 7, the production of fimbriae was strongly inhibited. Also, at pH values higher than 7, a decrease in production was observed. The consequences of the observed phenomena for the pathogenic behavior of this E. coli strain are discussed.
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Selected References
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- Burrows M. R., Sellwood R., Gibbons R. A. Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli. J Gen Microbiol. 1976 Oct;96(2):269–275. doi: 10.1099/00221287-96-2-269. [DOI] [PubMed] [Google Scholar]
- Contrepois M., Girardeau J. P., Gouet P., Der Vartanian M. Expression of K99 pilus of E. coli. Ann Rech Vet. 1983;14(4):400–407. [PubMed] [Google Scholar]
- Girardeau J. P., Dubourguier H. C., Gouet P. Effect of glucose and amino acids on expression of K99 antigen in Escherichia coli. J Gen Microbiol. 1982 Oct;128(10):2243–2249. doi: 10.1099/00221287-128-10-2243. [DOI] [PubMed] [Google Scholar]
- Girardeau J. P., Dubourguier H. C., Gouet P. Inhibition of K99 antigen synthesis by L-alanine enterotoxigenic Escherichia coli. J Gen Microbiol. 1982 Mar;128(3):463–470. doi: 10.1099/00221287-128-3-463. [DOI] [PubMed] [Google Scholar]
- Isaacson R. E. Factors affecting expression of the Escherichia coli pilus K99. Infect Immun. 1980 Apr;28(1):190–194. doi: 10.1128/iai.28.1.190-194.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isaacson R. E. Regulation of expression of Escherichia coli pilus K99. Infect Immun. 1983 May;40(2):633–639. doi: 10.1128/iai.40.2.633-639.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mooi F. R., de Graaf F. K., van Embden J. D. Cloning, mapping and expression of the genetic determinant that encodes for the K88ab antigen. Nucleic Acids Res. 1979 Mar;6(3):849–865. doi: 10.1093/nar/6.3.849. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moon H. W., Nagy B., Isaacson R. E., Orskov I. Occurrence of K99 antigen on Escherichia coli isolated from pigs and colonization of pig ileum by K99+ enterotoxigenic E. coli from calves and pigs. Infect Immun. 1977 Feb;15(2):614–620. doi: 10.1128/iai.15.2.614-620.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moon H. W. Pathogenesis of enteric diseases caused by Escherichia coli. Adv Vet Sci Comp Med. 1974;18(0):179–211. [PubMed] [Google Scholar]
- Stouthamer A. H., Bettenhaussen C. W. Determination of the efficiency of oxidative phosphorylation in continuous cultures of Aerobacter aerogenes. Arch Microbiol. 1975 Mar 10;102(3):187–192. doi: 10.1007/BF00428367. [DOI] [PubMed] [Google Scholar]
- de Graaf F. K., Klaasen-Boor P., van Hees J. E. Biosynthesis of the K99 surface antigen is repressed by alanine. Infect Immun. 1980 Oct;30(1):125–128. doi: 10.1128/iai.30.1.125-128.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Graaf F. K., Klemm P., Gaastra W. Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99. Infect Immun. 1981 Sep;33(3):877–883. doi: 10.1128/iai.33.3.877-883.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Graaf F. K., Krenn B. E., Klaasen P. Organization and expression of genes involved in the biosynthesis of K99 fimbriae. Infect Immun. 1984 Feb;43(2):508–514. doi: 10.1128/iai.43.2.508-514.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Graaf F. K., Roorda I. Production, purification, and characterization of the fimbrial adhesive antigen F41 isolated from calf enteropathogenic Escherichia coli strain B41M. Infect Immun. 1982 May;36(2):751–758. doi: 10.1128/iai.36.2.751-758.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Graaf F. K., Wientjes F. B., Klaasen-Boor P. Production of K99 antigen by enterotoxigenic Escherichia coli strains of antigen groups o8, o9, o20, and o101 grown at different conditions. Infect Immun. 1980 Jan;27(1):216–221. doi: 10.1128/iai.27.1.216-221.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Verseveld H. W., Chesbro W. R., Braster M., Stouthamer A. H. Eubacteria have 3 growth modes keyed to nutrient flow. Consequences for the concept of maintenance and maximal growth yield. Arch Microbiol. 1984 Feb;137(2):176–184. doi: 10.1007/BF00414463. [DOI] [PubMed] [Google Scholar]