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. 2009 Jan;181(1):65–79. doi: 10.1534/genetics.108.095513

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Copyright © 2009 by the Genetics Society of America

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Figure 7.— — Pds1p does not associate with chromatin during recombination. REC8-HA-tagged SK1/W303 strain (KCY392) harboring the Pds1p-FLAG expression plasmid (pKC7000) was grown to mid-log phase at 30° in synthetic acetate medium and then transferred to SPM and incubated at the same temperature. Samples were taken at the time points indicated (in hours) and whole-cell lysates (W) fractionated through a sucrose cushion to generate a chromatin bound pellet (P) and unbound supernatant (S) were analyzed by Western blotting and probed for the presence of Pds1p-FLAG (arrows, top) or Rec8p-HA (arrow, bottom). Molecular weight markers (in kilodaltons) are given on the left.