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. 1985 Oct;50(1):250–254. doi: 10.1128/iai.50.1.250-254.1985

Phagocytosis of staphylococci by human polymorphonuclear leukocytes is enhanced in the presence of endothelial cells.

C M Vandenbroucke-Grauls, H M Thijssen, J Verhoef
PMCID: PMC262164  PMID: 4044039

Abstract

The role of various surfaces in the phagocytosis of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) was studied. Uptake of both opsonized and unopsonized staphylococci on the surface of a monolayer of human venous endothelial cells was compared with uptake on an inert plastic surface, with an assay that uses radiolabeled bacteria. Uptake of unopsonized S. aureus was threefold higher on the endothelial cell surface than on the plastic surface and was followed by efficient killing of the phagocytosed staphylococci. Uptake of unopsonized S. aureus on endothelial cells was not inhibited by treatment of the PMN with pronase or 2-deoxy-D-glucose and was only partially inhibited by cytochalasin B treatment of the PMN. The supporting effect of endothelial cells on the phagocytosis of unopsonized S. aureus was not due to opsonization of the bacteria by immunoglobulin or complement from the endothelial cell surface, nor to coating with fibronectin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bevilacqua M. P., Amrani D., Mosesson M. W., Bianco C. Receptors for cold-insoluble globulin (plasma fibronectin) on human monocytes. J Exp Med. 1981 Jan 1;153(1):42–60. doi: 10.1084/jem.153.1.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Crofton R. W., Diesselhoff-den Dulk M. M., van Furth R. The origin, kinetics, and characteristics of the Kupffer cells in the normal steady state. J Exp Med. 1978 Jul 1;148(1):1–17. doi: 10.1084/jem.148.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eriksen H. O., Espersen F., Clemmensen I. Opsonic activity of fibronectin in the phagocytosis of Staphylococcus aureus by polymorphonuclear leukocytes. Eur J Clin Microbiol. 1984 Apr;3(2):108–112. doi: 10.1007/BF02014326. [DOI] [PubMed] [Google Scholar]
  4. Gudewicz P. W., Beezhold D. H., Van Alten P., Molnar J. Lack of stimulation of post-phagocytic metabolic activities of polymorphonuclear leukocytes by fibronectin opsonized particles. J Reticuloendothel Soc. 1982 Aug;32(2):143–154. [PubMed] [Google Scholar]
  5. Hed J., Stendahl O. Differences in the ingestion mechanisms of IgG and C3b particles in phagocytosis by neutrophils. Immunology. 1982 Apr;45(4):727–736. [PMC free article] [PubMed] [Google Scholar]
  6. Husband A. J., Brandon M. R., Lascelles A. K. Absorption and endogenous production of immunoglobulins in calves. Aust J Exp Biol Med Sci. 1972 Aug;50(4):491–498. doi: 10.1038/icb.1972.41. [DOI] [PubMed] [Google Scholar]
  7. JAMES R. C., MACLEOD C. J. Induction of staphylococcal infections in mice with small inocula introduced on sutures. Br J Exp Pathol. 1961 Jun;42:266–277. [PMC free article] [PubMed] [Google Scholar]
  8. Jaffe E. A., Hoyer L. W., Nachman R. L. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest. 1973 Nov;52(11):2757–2764. doi: 10.1172/JCI107471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jaffe E. A., Mosher D. F. Synthesis of fibronectin by cultured human endothelial cells. J Exp Med. 1978 Jun 1;147(6):1779–1791. doi: 10.1084/jem.147.6.1779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kloster F. E. Complications of artificial heart valves. JAMA. 1979 May 18;241(20):2201–2203. [PubMed] [Google Scholar]
  12. Lee D. A., Hoidal J. R., Clawson C. C., Quie P. G., Peterson P. K. Phagocytosis by polymorphonuclear leukocytes of Staphylococcus aureus and Pseudomonas aeruginosa adherent to plastic, agar, or glass. J Immunol Methods. 1983 Sep 30;63(1):103–114. doi: 10.1016/0022-1759(83)90213-2. [DOI] [PubMed] [Google Scholar]
  13. Lee D. A., Hoidal J. R., Garlich D. J., Clawson C. C., Quie P. G., Peterson P. K. Opsonin-independent phagocytosis of surface-adherent bacteria by human alveolar macrophages. J Leukoc Biol. 1984 Dec;36(6):689–701. doi: 10.1002/jlb.36.6.689. [DOI] [PubMed] [Google Scholar]
  14. Noble W. C. The production of subcutaneous staphylococcal skin lesions in mice. Br J Exp Pathol. 1965 Jun;46(3):254–262. [PMC free article] [PubMed] [Google Scholar]
  15. Pommier C. G., O'Shea J., Chused T., Yancey K., Frank M. M., Takahashi T., Brown E. J. Studies on the fibronectin receptors of human peripheral blood leukocytes. Morphologic and functional characterization. J Exp Med. 1984 Jan 1;159(1):137–151. doi: 10.1084/jem.159.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Proctor R. A., Prendergast E., Mosher D. F. Fibronectin mediates attachment of Staphylococcus aureus to human neutrophils. Blood. 1982 Apr;59(4):681–687. [PubMed] [Google Scholar]
  17. Reinders J. H., De Groot P. G., Gonsalves M. D., Zandbergen J., Loesberg C., Van Mourik J. A. Isolation of a storage and secretory organelle containing Von Willebrand protein from cultured human endothelial cells. Biochim Biophys Acta. 1984 Jul 20;804(3):361–369. doi: 10.1016/0167-4889(84)90140-x. [DOI] [PubMed] [Google Scholar]
  18. Van de Water L., Destree A. T., Hynes R. O. Fibronectin binds to some bacteria but does not promote their uptake by phagocytic cells. Science. 1983 Apr 8;220(4593):201–204. doi: 10.1126/science.6338594. [DOI] [PubMed] [Google Scholar]
  19. Vandenbroucke-Grauls C. M., Thijssen H. M., Verhoef J. Interaction between human polymorphonuclear leucocytes and Staphylococcus aureus in the presence and absence of opsonins. Immunology. 1984 Jul;52(3):427–435. [PMC free article] [PubMed] [Google Scholar]
  20. Verbrugh H. A., Peters R., Peterson P. K., Verhoef J. Phagocytosis and killing of staphylococci by human polymorphonuclear and mononuclear leucocytes. J Clin Pathol. 1978 Jun;31(6):539–545. doi: 10.1136/jcp.31.6.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Verbrugh H. A., Peterson P. K., Smith D. E., Nguyen B. Y., Hoidal J. R., Wilkinson B. J., Verhoef J., Furcht L. T. Human fibronectin binding to staphylococcal surface protein and its relative inefficiency in promoting phagocytosis by human polymorphonuclear leukocytes, monocytes, and alveolar macrophages. Infect Immun. 1981 Sep;33(3):811–819. doi: 10.1128/iai.33.3.811-819.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Verbrugh H. A., Van Dijk W. C., Peters R., Van Der Tol M. E., Peterson P. K., Verhoef J. Staphylococcus aureus opsonization mediated via the classical and alternative complement pathways. A kinetic study using MgEGTA chelated serum and human sera deficient in IgG and complement factors C1s and C2. Immunology. 1979 Mar;36(3):391–397. [PMC free article] [PubMed] [Google Scholar]
  23. Verhoef J., Peterson P. K., Quie P. G. Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using [3H]thymidine labeled bacteria. J Immunol Methods. 1977;14(3-4):303–311. doi: 10.1016/0022-1759(77)90141-7. [DOI] [PubMed] [Google Scholar]
  24. Verhoef J., Peterson P., Kim Y., Sabath L. D., Quie P. G. Opsonic requirements for staphylococcal phagocytosis. Heterogeneity among strains. Immunology. 1977 Aug;33(2):191–197. [PMC free article] [PubMed] [Google Scholar]
  25. Wright S. D., Silverstein S. C. Receptors for C3b and C3bi promote phagocytosis but not the release of toxic oxygen from human phagocytes. J Exp Med. 1983 Dec 1;158(6):2016–2023. doi: 10.1084/jem.158.6.2016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zimmerli W., Lew P. D., Waldvogel F. A. Pathogenesis of foreign body infection. Evidence for a local granulocyte defect. J Clin Invest. 1984 Apr;73(4):1191–1200. doi: 10.1172/JCI111305. [DOI] [PMC free article] [PubMed] [Google Scholar]

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