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. 1985 Oct;50(1):255–261. doi: 10.1128/iai.50.1.255-261.1985

Experimental enterotoxin-induced Escherichia coli diarrhea and protection induced by previous infection with bacteria of the same adhesin or enterotoxin type.

C M Ahrén, A M Svennerholm
PMCID: PMC262165  PMID: 2864313

Abstract

The diarrheal response to an initial and a second infection with Escherichia coli expressing various enterotoxins (the heat-stable toxin [ST] alone or in combination with the heat-labile toxin [LT]) and colonization factor antigens (CFA/I, CFA/II, or E8775-type) was studied in the reversible tie adult rabbit diarrhea model. An initial infection with high doses (1 X 10(10) to 5 X 10(11) bacteria) of the various strains regularly induced diarrhea which was usually self-limiting (only 7 of 85 animals died). The diarrheal response to equally effective doses of different strains producing both ST and LT (ST/LT) did not differ significantly with serotype or colonization factor antigen. ST/LT-producing strains appeared to induce severe disease more regularly than ST-producing strains carrying the same adhesin. Previous infection with CFA/I-carrying, ST/LT-producing E. coli protected all animals reinfected with an otherwise highly diarrheogenic dose of the same strain as well as against challenge with a CFA/I-carrying, ST/LT-producing strain with different O-, K-, and H-antigens. Fecal excretion of bacteria was also significantly reduced in the protected animals, although not completely eliminated. When only one of the two antigens, CFA/I and LT, was shared by the immunizing and rechallenge strains, partial protection was evident consistent with independent antibacterial (anti-CFA) and antitoxic (anti-LT) immune mechanisms. Oral immunization with purified CFA/I significantly reduced fluid secretion in intestinal loops infected with CFA/I-carrying enterotoxigenic bacteria.

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Selected References

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  1. Acres S. D., Isaacson R. E., Babiuk L. A., Kapitany R. A. Immunization of calves against enterotoxigenic colibacillosis by vaccinating dams with purified K99 antigen and whole cell bacterins. Infect Immun. 1979 Jul;25(1):121–126. doi: 10.1128/iai.25.1.121-126.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ahrén C. M., Svennerholm A. M. Synergistic protective effect of antibodies against Escherichia coli enterotoxin and colonization factor antigens. Infect Immun. 1982 Oct;38(1):74–79. doi: 10.1128/iai.38.1.74-79.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bijlsma I. G., de Nijs A., van der Meer C., Frik J. F. Different pig phenotypes affect adherence of Escherichia coli to jejunal brush borders by K88ab, K88ac, or K88ad antigen. Infect Immun. 1982 Sep;37(3):891–894. doi: 10.1128/iai.37.3.891-894.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Black R. E., Merson M. H., Rahman A. S., Yunus M., Alim A. R., Huq I., Yolken R. H., Curlin G. T. A two-year study of bacterial, viral, and parasitic agents associated with diarrhea in rural Bangladesh. J Infect Dis. 1980 Nov;142(5):660–664. doi: 10.1093/infdis/142.5.660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cray W. C., Jr, Tokunaga E., Pierce N. F. Successful colonization and immunization of adult rabbits by oral inoculation with Vibrio cholerae O1. Infect Immun. 1983 Aug;41(2):735–741. doi: 10.1128/iai.41.2.735-741.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DIXON J. M. The fate of bacteria in the small intestine. J Pathol Bacteriol. 1960 Jan;79:131–140. doi: 10.1002/path.1700790116. [DOI] [PubMed] [Google Scholar]
  7. Darfeuille A., Lafeuille B., Joly B., Cluzel R. A new colonization factor antigen (CFA/III) produced by enteropathogenic Escherichia coli O128:B12. Ann Microbiol (Paris) 1983 Jan-Feb;134A(1):53–64. doi: 10.1016/0769-2609(83)90103-5. [DOI] [PubMed] [Google Scholar]
  8. Deetz T. R., Evans D. J., Jr, Evans D. G., DuPont H. L. Serologic responses to somatic O and colonization-factor antigens of enterotoxigenic Escherichia coli in travelers. J Infect Dis. 1979 Jul;140(1):114–118. doi: 10.1093/infdis/140.1.114. [DOI] [PubMed] [Google Scholar]
  9. Deneke C. F., Thorne G. M., Gorbach S. L. Serotypes of attachment pili of enterotoxigenic Escherichia coli isolated from humans. Infect Immun. 1981 Jun;32(3):1254–1260. doi: 10.1128/iai.32.3.1254-1260.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Evans D. G., Evans D. J., Jr, Clegg S., Pauley J. A. Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun. 1979 Aug;25(2):738–748. doi: 10.1128/iai.25.2.738-748.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Evans D. G., Evans D. J., Jr New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infect Immun. 1978 Aug;21(2):638–647. doi: 10.1128/iai.21.2.638-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Evans D. G., Evans D. J., Jr, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977 Nov;18(2):330–337. doi: 10.1128/iai.18.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Evans D. G., Graham D. Y., Evans D. J., Jr Administration of purified colonization factor antigens (CFA/I, CFA/II) of enterotoxigenic Escherichia coli to volunteers. Response to challenge with virulent enterotoxigenic Escherichia coli. Gastroenterology. 1984 Oct;87(4):934–940. [PubMed] [Google Scholar]
  14. Evans D. G., Silver R. P., Evans D. J., Jr, Chase D. G., Gorbach S. L. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. Infect Immun. 1975 Sep;12(3):656–667. doi: 10.1128/iai.12.3.656-667.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gaastra W., de Graaf F. K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev. 1982 Jun;46(2):129–161. doi: 10.1128/mr.46.2.129-161.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Holmgren J., Svennerholm A. M., Ouchterlony O., Anderson A., Walletström G., Westerberg-Berndtsson U. Antitoxic immunity in experimental cholera: protection, and serum and local antibody responses in rabbits after enteral and parenteral immunization. Infect Immun. 1975 Dec;12(6):1331–1340. doi: 10.1128/iai.12.6.1331-1340.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Honda T., Arita M., Miwatani T. Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli: a possible new colonization factor. Infect Immun. 1984 Mar;43(3):959–965. doi: 10.1128/iai.43.3.959-965.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Klipstein F. A., Engert R. F., Clements J. D. Development of a vaccine of cross-linked heat-stable and heat-labile enterotoxins that protects against Escherichia coli producing either enterotoxin. Infect Immun. 1982 Aug;37(2):550–557. doi: 10.1128/iai.37.2.550-557.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Levine M. M., Nalin D. R., Hoover D. L., Bergquist E. J., Hornick R. B., Young C. R. Immunity to enterotoxigenic Escherichia coli. Infect Immun. 1979 Mar;23(3):729–736. doi: 10.1128/iai.23.3.729-736.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Levine M. M., Ristaino P., Marley G., Smyth C., Knutton S., Boedeker E., Black R., Young C., Clements M. L., Cheney C. Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans. Infect Immun. 1984 May;44(2):409–420. doi: 10.1128/iai.44.2.409-420.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Morgan R. L., Isaacson R. E., Moon H. W., Brinton C. C., To C. C. Immunization of suckling pigs against enterotoxigenic Escherichia coli-induced diarrheal disease by vaccinating dams with purified 987 or K99 pili: protection correlates with pilus homology of vaccine and challenge. Infect Immun. 1978 Dec;22(3):771–777. doi: 10.1128/iai.22.3.771-777.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sack R. B., Froehlich J. L. Antigenic similarity of heat-labile enterotoxins from diverse strains of Escherichia coli. J Clin Microbiol. 1977 Jun;5(6):570–572. doi: 10.1128/jcm.5.6.570-572.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Spira W. M., Sack R. B., Froehlich J. L. Simple adult rabbit model for Vibrio cholerae and enterotoxigenic Escherichia coli diarrhea. Infect Immun. 1981 May;32(2):739–747. doi: 10.1128/iai.32.2.739-747.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Svennerholm A. M., Ahrén C. Immune protection against enterotoxinogenic E. coli: search for synergy between antibodies to enterotoxin and somatic antigens. Acta Pathol Microbiol Immunol Scand C. 1982 Feb;90(1):1–6. doi: 10.1111/j.1699-0463.1982.tb01409.x. [DOI] [PubMed] [Google Scholar]
  25. Svennerholm A. M., Ahrén C. Serological subtypes of Escherichia coli colonization factor antigen II. Eur J Clin Microbiol. 1982 Apr;1(2):107–111. doi: 10.1007/BF02014201. [DOI] [PubMed] [Google Scholar]
  26. Thomas L. V., Cravioto A., Scotland S. M., Rowe B. New fimbrial antigenic type (E8775) that may represent a colonization factor in enterotoxigenic Escherichia coli in humans. Infect Immun. 1982 Mar;35(3):1119–1124. doi: 10.1128/iai.35.3.1119-1124.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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