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. 1985 Oct;50(1):310–316. doi: 10.1128/iai.50.1.310-316.1985

Brucella fractions behave as nonspecific mitogens and polyclonal B-cell activators for human lymphocytes.

J P Vendrell, H Rabesandratana, M F Huguet, A Cannat, A Serre
PMCID: PMC262173  PMID: 3876286

Abstract

Two lipid-A-free fractions which were extracted from Brucella melitensis and were designated PI and SF stimulated human unsensitized mononuclear cells to proliferate and to secrete immunoglobulins. Both of these effects were observed in cultures of peripheral blood, tonsils, and cord blood lymphocytes. Neither B cells nor T cells alone proliferated in the presence of these fractions, whereas the proliferative response of T cells plus B cells was largely independent of accessory cells. Polyclonal activation was estimated by counting the cells which secreted immunoglobulins of different isotypes into culture supernatants. This phenomenon was strongly T dependent.

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Selected References

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