Figure 1.

Mice immunized with gp75/Sf9 produced antibodies against gp75 in syngeneic melanocytic cells. C57BL/6 mice were immunized with lysates of insect Sf9 cells infected with either recombinant baculovirus expressing syngeneic mouse gp75 (gp75/Sf9) or wt baculovirus (wt/Sf9). Mice were immunized four times subcutaneously with freeze–thawed Sf9 cells, 5 × 10⁶ cells per immunization. Sera from immunized mice were used to immunoprecipitate syngeneic gp75 from [³⁵S]methionine-labeled B16 melanoma lysates. The mAb TA99 was used as a positive control (right lane). Results for five mice in each group are shown (lanes 1–5).