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. 1986 Apr;52(1):236–239. doi: 10.1128/iai.52.1.236-239.1986

Ontogeny of macrophage function to release superoxide anion in conventional and germfree mice.

M Mitsuyama, R Ohara, K Amako, K Nomoto, T Yokokura, K Nomoto
PMCID: PMC262225  PMID: 3007361

Abstract

To determine whether the presence of bacterial flora contributes to the ontogenic development of macrophage function, the ability of macrophages to release superoxide anion (O2-) in response to stimulation with phorbol myristate acetate was compared in conventional and germfree mice of various ages after birth. One-week-old conventional mice showed a very low level of O2- release by their macrophages, and gradual increases were observed in 2-, 3-, and 4-week-old mice in an age-dependent manner. Macrophages from germfree mice always showed a significantly lower level of O2- release compared with conventional mice of the same age; however, age-dependent functional development was seen also in the germfree group. The poor level of O2- release by macrophages from adult germfree mice could be restored to more than the level by conventional mice when the mice were conventionalized for 3 weeks. These results suggested that the ontogenic development of macrophage function is not controlled by the presence of bacterial flora but that the full-scale expression of function at each age is under the influence of microflora.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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