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. 1986 Apr;52(1):309–313. doi: 10.1128/iai.52.1.309-313.1986

Inhibition of mitogenesis induced by phytohemagglutinin and Lens culinaris lectin in adherent-cell supernatants treated with protein extract of Mycobacterium tuberculosis.

C Parra, L F Montaño, M Huesca, I Rayón, K Willms, F Goodsaid
PMCID: PMC262235  PMID: 3082760

Abstract

Specific stimulation of T cells by phytohemagglutinin and Lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (CFPE) derived from bacterial cultures of Mycobacterium tuberculosis H37Ra (avirulent) and H37Rv (virulent). The induction of the inhibitory factor was blocked by the presence of hyperimmune antisera to H37Rv or H37Ra CFPE. The inhibitory factor did not seem to be a CFPE reprocessed by the adherent cells. Inhibitory activity was maximal in supernatants of adherent-cell cultures incubated for 48 h; the inhibitory factor was heat labile, and its production was dependent on the concentration of M. tuberculosis CFPE. A mouse monocyte-macrophage cell line, ATCC J774A.1, produced an identical inhibitory factor, thus excluding a non-macrophage-contaminating adherent cell as the source of the factor. This inhibitory factor also interfered with the recognition of phytohemagglutinin and Lens culinaris lectin by T cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J. A., Rao V. S., Mitchell M. S. Systemic bacillus Calmette-Guérin (BCG) activates natural suppressor cells. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5142–5144. doi: 10.1073/pnas.75.10.5142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bhatnagar R., Malaviya A. N., Narayanan S., Rajgopalan P., Kumar R., Bharadwaj O. P. Spectrum of immune response abnormalities in different clinical forms of tuberculosis. Am Rev Respir Dis. 1977 Feb;115(2):207–212. doi: 10.1164/arrd.1977.115.2.207. [DOI] [PubMed] [Google Scholar]
  3. Bona C., Audibert F., Juy D., Chedid L. Cell suppression in PPD-induced blast specific response of human peripheral blood lymphocytes. Clin Exp Immunol. 1976 Nov;26(2):258–266. [PMC free article] [PubMed] [Google Scholar]
  4. Bonnard G. D., Yasaka K., Maca R. D. Continued growth of functional human T lymphocytes: production of human T-cell growth factor. Cell Immunol. 1980 May;51(2):390–401. doi: 10.1016/0008-8749(80)90270-1. [DOI] [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Regulation of cellular and humoral immune responses by T-cell subclasses. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):23–32. doi: 10.1101/sqb.1977.041.01.006. [DOI] [PubMed] [Google Scholar]
  6. Cantor H., Gershon R. K. Immunological circuits: cellular composition. Fed Proc. 1979 Jun;38(7):2058–2064. [PubMed] [Google Scholar]
  7. Daniel T. M., Good R. C., Janicki B. W. Immunoelectrophoresis of Mycobacterium tuberculosis antigens. Comparative analysis of cell extract and culture filtrate antigens. Am Rev Respir Dis. 1975 Nov;112(5):639–644. doi: 10.1164/arrd.1975.112.5.639. [DOI] [PubMed] [Google Scholar]
  8. Ellner J. J., Daniel T. M. Immunosuppression by mycobacterial arabinomannan. Clin Exp Immunol. 1979 Feb;35(2):250–257. [PMC free article] [PubMed] [Google Scholar]
  9. Ellner J. J. Suppressor adherent cells in human tuberculosis. J Immunol. 1978 Dec;121(6):2573–2579. [PubMed] [Google Scholar]
  10. Fischer A., Durandy A., Griscelli C. Role of prostaglandin E2 in the induction of nonspecific T lymphocyte suppressor activity. J Immunol. 1981 Apr;126(4):1452–1455. [PubMed] [Google Scholar]
  11. GOLD A. M., FAHRNEY D. E. The mechanism of reactivation of phenylmethanesulfonyl alpha-chymotrypsin. Biochem Biophys Res Commun. 1963 Jan 18;10:55–59. doi: 10.1016/0006-291x(63)90267-5. [DOI] [PubMed] [Google Scholar]
  12. Goren M. B. Mycobacterial lipids: selected topics. Bacteriol Rev. 1972 Mar;36(1):33–64. doi: 10.1128/br.36.1.33-64.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holden M., Dubin M. R., Diamond P. H. Frequency of negative intermediate-strength tuberculin sensitivity in patients with active tuberculosis. N Engl J Med. 1971 Dec 30;285(27):1506–1509. doi: 10.1056/NEJM197112302852704. [DOI] [PubMed] [Google Scholar]
  14. Katz D. H. Adaptive differentiation of murine lymphocytes: implications for mechanisms of cell--cell recognition and the regulation of immune responses. Fed Proc. 1979 Jun;38(7):2065–2070. [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Leclerc C., Morin A., Deriaud E., Chedid L. Inhibition of human IL 2 production by MDP and derivatives. J Immunol. 1984 Oct;133(4):1996–2000. [PubMed] [Google Scholar]
  17. Mizel S. B. Interleukin 1 and T cell activation. Immunol Rev. 1982;63:51–72. doi: 10.1111/j.1600-065x.1982.tb00411.x. [DOI] [PubMed] [Google Scholar]
  18. Oppenheim J. J., Togawa A., Chedid L., Mizel S. Components of mycobacteria and muramyl dipeptide with adjuvant activity induce lymphocyte activating factor. Cell Immunol. 1980 Mar 1;50(1):71–81. doi: 10.1016/0008-8749(80)90007-6. [DOI] [PubMed] [Google Scholar]
  19. Ralph P., Nakoinz I. Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line. Nature. 1975 Oct 2;257(5525):393–394. doi: 10.1038/257393a0. [DOI] [PubMed] [Google Scholar]
  20. Wadee A. A., Sher R., Rabson A. R. Production of a suppressor factor by human adherent cells treated with mycobacteria. J Immunol. 1980 Sep;125(3):1380–1386. [PubMed] [Google Scholar]

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