Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1986 Apr;52(1):337–340. doi: 10.1128/iai.52.1.337-340.1986

Antigenic variation in the phase I lipopolysaccharide of Coxiella burnetii isolates.

T Hackstadt
PMCID: PMC262242  PMID: 3957431

Abstract

Coxiella burnetii isolates from a variety of clinical and geographical sources were screened for antigenic variation of lipopolysaccharides (LPSs) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis coupled with silver staining or immunoblotting. All isolates from chronic Q fever or other sources possessed a phase I-type LPS. These LPSs appeared to fall into three groups based on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile or on reactivity with rabbit anti-C. burnetti antisera. The LPS of one group was identified on isolates from milk, ticks, or primary Q fever. The two remaining groups were found almost exclusively on isolates from human cases of chronic Q fever.

Full text

PDF
337

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ascher M. S., Berman M. A., Ruppanner R. Initial clinical and immunologic evaluation of a new phase I Q fever vaccine and skin test in humans. J Infect Dis. 1983 Aug;148(2):214–222. doi: 10.1093/infdis/148.2.214. [DOI] [PubMed] [Google Scholar]
  2. Baca O. G., Paretsky D. Q fever and Coxiella burnetii: a model for host-parasite interactions. Microbiol Rev. 1983 Jun;47(2):127–149. doi: 10.1128/mr.47.2.127-149.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brezina R., Pospísil V. Study of the antigenic structure of Coxiella burneti. 8. Immunogenicity of phenol-extracted phase I antigenic component. Acta Virol. 1970 Jul;14(4):302–306. [PubMed] [Google Scholar]
  4. Fiset P., Ormsbee R. A., Silberman R., Peacock M., Spielman S. H. A microagglutination technique for detection and measurement of rickettsial antibodies. Acta Virol. 1969 Jan;13(1):60–66. [PubMed] [Google Scholar]
  5. Fiset P., Wike D. A., Pickens E. G., Ormsbee R. A. An antigenic comparison of strains of Coxiella burneti. Acta Virol. 1971 Mar;15(2):161–166. [PubMed] [Google Scholar]
  6. Goldman R. C., Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem. 1980;107(1):145–153. doi: 10.1111/j.1432-1033.1980.tb04635.x. [DOI] [PubMed] [Google Scholar]
  7. Hackstadt T., Peacock M. G., Hitchcock P. J., Cole R. L. Lipopolysaccharide variation in Coxiella burnetti: intrastrain heterogeneity in structure and antigenicity. Infect Immun. 1985 May;48(2):359–365. doi: 10.1128/iai.48.2.359-365.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hackstadt T., Todd W. J., Caldwell H. D. Disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of chlamydiae? J Bacteriol. 1985 Jan;161(1):25–31. doi: 10.1128/jb.161.1.25-31.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kazár J., Brezina R., Palanová A., Tvrdá B., Schramek S. Immunogenicity and reactogenicity of a Q fever chemovaccine in persons professionally exposed to Q fever in Czechoslovakia. Bull World Health Organ. 1982;60(3):389–394. [PMC free article] [PubMed] [Google Scholar]
  11. LUOTO L., BELL J. F., CASEY M., LACKMAN D. B. Q FEVER VACCINATION OF HUMAN VOLUNTEERS. I. THE SEROLOGIC AND SKIN-TEST RESPONSE FOLLOWING SUBCUTANEOUS INJECTIONS. Am J Hyg. 1963 Jul;78:1–15. [PubMed] [Google Scholar]
  12. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marmion B. P., Ormsbee R. A., Kyrkou M., Wright J., Worswick D., Cameron S., Esterman A., Feery B., Collins W. Vaccine prophylaxis of abattoir-associated Q fever. Lancet. 1984 Dec 22;2(8417-8418):1411–1414. doi: 10.1016/s0140-6736(84)91617-9. [DOI] [PubMed] [Google Scholar]
  14. Munford R. S., Hall C. L., Rick P. D. Size heterogeneity of Salmonella typhimurium lipopolysaccharides in outer membranes and culture supernatant membrane fragments. J Bacteriol. 1980 Nov;144(2):630–640. doi: 10.1128/jb.144.2.630-640.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ORMSBEE R. A., BELL E. J., LACKMAN D. B., TALLENT G. THE INFLUENCE OF PHASE ON THE PROTECTIVE POTENCY OF Q FEVER VACCINE. J Immunol. 1964 Mar;92:404–412. [PubMed] [Google Scholar]
  16. ORMSBEE R. A., PICKENS E. G., LACKMAN D. B. AN ANTIGENIC ANALYSIS OF THREE STRAINS OF COXIELLA BURNETI. Am J Hyg. 1964 Mar;79:154–162. doi: 10.1093/oxfordjournals.aje.a120371. [DOI] [PubMed] [Google Scholar]
  17. Palmer S. R., Young S. E. Q-fever endocarditis in England and Wales, 1975-81. Lancet. 1982 Dec 25;2(8313):1448–1449. doi: 10.1016/s0140-6736(82)91341-1. [DOI] [PubMed] [Google Scholar]
  18. Palva E. T., Mäkelä P. H. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Eur J Biochem. 1980;107(1):137–143. doi: 10.1111/j.1432-1033.1980.tb04634.x. [DOI] [PubMed] [Google Scholar]
  19. Peacock M. G., Philip R. N., Williams J. C., Faulkner R. S. Serological evaluation of O fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis. Infect Immun. 1983 Sep;41(3):1089–1098. doi: 10.1128/iai.41.3.1089-1098.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. STOKER M. G., FISET P. Phase variation of the Nine Mile and other strains of Rickettsia burneti. Can J Microbiol. 1956 May;2(3):310–321. doi: 10.1139/m56-036. [DOI] [PubMed] [Google Scholar]
  21. Samuel J. E., Frazier M. E., Mallavia L. P. Correlation of plasmid type and disease caused by Coxiella burnetii. Infect Immun. 1985 Sep;49(3):775–779. doi: 10.1128/iai.49.3.775-779.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schramek S., Mayer H. Different sugar compositions of lipopolysaccharides isolated from phase I and pure phase II cells of Coxiella burnetii. Infect Immun. 1982 Oct;38(1):53–57. doi: 10.1128/iai.38.1.53-57.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schramek S., Radziejewska-Lebrecht J., Mayer H. 3-C-branched aldoses in lipopolysaccharide of phase I Coxiella burnetii and their role as immunodominant factors. Eur J Biochem. 1985 May 2;148(3):455–461. doi: 10.1111/j.1432-1033.1985.tb08861.x. [DOI] [PubMed] [Google Scholar]
  24. Tobin M. J., Cahill N., Gearty G., Maurer B., Blake S., Daly K., Hone R. Q fever endocarditis. Am J Med. 1982 Mar;72(3):396–400. doi: 10.1016/0002-9343(82)90495-8. [DOI] [PubMed] [Google Scholar]
  25. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  26. Turck W. P., Howitt G., Turnberg L. A., Fox H., Longson M., Matthews M. B., Das Gupta R. Chronic Q fever. Q J Med. 1976 Apr;45(178):193–217. [PubMed] [Google Scholar]
  27. VIVONA S., LOWENTHAL J. P., BERMAN S., BENENSON A. S., SMADEL J. E. REPORT OF A FIELD STUDY WITH Q FEVER VACCINE. Am J Hyg. 1964 Mar;79:143–153. doi: 10.1093/oxfordjournals.aje.a120370. [DOI] [PubMed] [Google Scholar]
  28. Williams J. C., Peacock M. G., McCaul T. F. Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components. Infect Immun. 1981 May;32(2):840–851. doi: 10.1128/iai.32.2.840-851.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES