Abstract
The present study shows adherence of Treponema denticola L12D and Treponema vincentii RitzA to guinea pig ear epithelial cells in vitro. The number of adhering organisms was positively related with the treponemal concentration and contact time. Incubation of washed T. denticola L12D and T. vincentii RitzA organisms or their culture supernatants with the epithelial cells induced morphological damage and detachment of these cells. In addition, indications for inhibition of epithelial cell proliferation were found. The activity of the supernatants was dose dependent, heat sensitive, and sensitive to sulfhydryl-containing components, suggesting the presence of enzymatic activity that might be of importance in the pathogenicity of these oral treponemes.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Armitage G. C., Dickinson W. R., Jenderseck R. S., Levine S. M., Chambers D. W. Relationship between the percentage of subgingival spirochetes and the severity of periodontal disease. J Periodontol. 1982 Sep;53(9):550–556. doi: 10.1902/jop.1982.53.9.550. [DOI] [PubMed] [Google Scholar]
- Blakemore R. P., Canale-Parola E. Arginine catabolism by Treponema denticola. J Bacteriol. 1976 Nov;128(2):616–622. doi: 10.1128/jb.128.2.616-622.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boehringer H., Taichman N. S., Shenker B. J. Suppression of fibroblast proliferation by oral spirochetes. Infect Immun. 1984 Jul;45(1):155–159. doi: 10.1128/iai.45.1.155-159.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown J. E., Rothman S. W., Doctor B. P. Inhibition of protein synthesis in intact HeLa cells by Shigella dysenteriae 1 toxin. Infect Immun. 1980 Jul;29(1):98–107. doi: 10.1128/iai.29.1.98-107.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitzgerald T. J., Johnson R. C., Miller J. N., Sykes J. A. Characterization of the attachment of Treponema pallidum (Nichols strain) to cultured mammalian cells and the potential relationship of attachment to pathogenicity. Infect Immun. 1977 Nov;18(2):467–478. doi: 10.1128/iai.18.2.467-478.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitzgerald T. J., Miller J. N., Sykes J. A. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. Infect Immun. 1975 May;11(5):1133–1140. doi: 10.1128/iai.11.5.1133-1140.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitzgerald T. J. Pathogenesis and immunology of Treponema pallidum. Annu Rev Microbiol. 1981;35:29–54. doi: 10.1146/annurev.mi.35.100181.000333. [DOI] [PubMed] [Google Scholar]
- Fitzgerald T. J., Repesh L. A., Oakes S. G. Morphological destruction of cultured cells by the attachment of Treponema pallidum. Br J Vener Dis. 1982 Feb;58(1):1–11. doi: 10.1136/sti.58.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frank R. M. Bacterial penetration in the apical pocket wall of advanced human periodontitis. J Periodontal Res. 1980 Nov;15(6):563–573. doi: 10.1111/j.1600-0765.1980.tb00315.x. [DOI] [PubMed] [Google Scholar]
- Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LISTGARTEN M. A. ELECTRON MICROSCOPIC OBSERVATIONS ON THE BACTERIAL FLORA OF ACUTE NECROTIZING ULCERATIVE GINGIVITIS. J Periodontol. 1965 Jul-Aug;36:328–339. doi: 10.1902/jop.1965.36.4.328. [DOI] [PubMed] [Google Scholar]
- Listgarten M. A., Helldén L. Relative distribution of bacteria at clinically healthy and periodontally diseased sites in humans. J Clin Periodontol. 1978 May;5(2):115–132. doi: 10.1111/j.1600-051x.1978.tb01913.x. [DOI] [PubMed] [Google Scholar]
- Maltha J. C., Mikx F. H., Kuijpers F. J. Necrotizing ulcerative gingivitis in beagle dogs. III. Distribution of spirochetes in interdental gingival tissue. J Periodontal Res. 1985 Sep;20(5):522–531. doi: 10.1111/j.1600-0765.1985.tb00836.x. [DOI] [PubMed] [Google Scholar]
- Mikx F. H., Ngassapa D. N., Reijntjens F. M., Maltha J. C. Effect of splint placement on black-pigmented Bacteroides and spirochetes in the dental plaque of beagle dogs. J Dent Res. 1984 Nov;63(11):1284–1288. doi: 10.1177/00220345840630110601. [DOI] [PubMed] [Google Scholar]
- Mikx F. H., van Campen G. J. Microscopical evaluation of the microflora in relation to necrotizing ulcerative gingivitis in the beagle dog. J Periodontal Res. 1982 Nov;17(6):576–584. doi: 10.1111/j.1600-0765.1982.tb01179.x. [DOI] [PubMed] [Google Scholar]
- Olsen I. Attachment of Treponema denticola to cultured human epithelial cells. Scand J Dent Res. 1984 Feb;92(1):55–63. doi: 10.1111/j.1600-0722.1984.tb00860.x. [DOI] [PubMed] [Google Scholar]
- Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saglie F. R., Carranza F. A., Jr, Newman M. G., Cheng L., Lewin K. J. Identification of tissue-invading bacteria in human periodontal disease. J Periodontal Res. 1982 Sep;17(5):452–455. doi: 10.1111/j.1600-0765.1982.tb02024.x. [DOI] [PubMed] [Google Scholar]
- Syed S. A., Loesche W. J. Survival of human dental plaque flora in various transport media. Appl Microbiol. 1972 Oct;24(4):638–644. doi: 10.1128/am.24.4.638-644.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]