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. 1993 Jan;31(1):97–101. doi: 10.1128/jcm.31.1.97-101.1993

Evaluation of an enzyme immunoassay for detection of cryptococcal capsular polysaccharide antigen in serum and cerebrospinal fluid.

U K Frank 1, S L Nishimura 1, N C Li 1, K Sugai 1, D M Yajko 1, W K Hadley 1, V L Ng 1
PMCID: PMC262628  PMID: 8417038

Abstract

The Premier enzyme immunoassay (Meridian Diagnostics, Inc., Cincinnati, Ohio) was compared with a latex agglutination assay (CALAS; Meridian) for the ability to detect cryptococcal capsular polysaccharide antigen (CrAg) in serum and cerebrospinal fluid (CSF). A total of 594 specimens (471 serum samples and 123 CSF samples) obtained from 430 patients, most of whom were at risk for or had AIDS, were tested in parallel by both systems. Both tests were independently evaluated for their ability to (i) detect CrAg when used as a screening test and (ii) quantitate the CrAg present when used as a titration assay. Chart review to assess clinical outcome after the time of specimen collection was conducted for all patients. When both assays were used as screening assays, 103 serum samples and 18 CSF samples were positive and 356 serum samples and 104 CSF specimens were negative by both assays (97.8% concordance). Thirteen specimens (12 serum samples, 1 CSF sample) gave discrepant screening results. When the tests were used as semiquantitative assays for titer determinations, the CrAg titers determined by the enzyme immunoassay were generally higher than those obtained with the latex agglutination assay. In summary, results obtained with the enzyme immunoassay correlated well with those obtained with the latex agglutination test for screening for the presence of CrAg and for determining the titer of CrAg in serum or CSF.

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Selected References

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  1. BLOOMFIELD N., GORDON M. A., ELMENDORF D. F., Jr DETECTION OF CRYPTOCOCCUS NEOFORMANS ANTIGEN IN BODY FLUIDS BY LATEX PARTICLE AGGLUTINATION. Proc Soc Exp Biol Med. 1963 Oct;114:64–67. doi: 10.3181/00379727-114-28586. [DOI] [PubMed] [Google Scholar]
  2. Bennett J. E., Bailey J. W. Control for rheumatoid factor in the latex test for cryptococcosis. Am J Clin Pathol. 1971 Sep;56(3):360–365. doi: 10.1093/ajcp/56.3.360. [DOI] [PubMed] [Google Scholar]
  3. Boom W. H., Piper D. J., Ruoff K. L., Ferraro M. J. New cause for false-positive results with the cryptococcal antigen test by latex agglutination. J Clin Microbiol. 1985 Nov;22(5):856–857. doi: 10.1128/jcm.22.5.856-857.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chuck S. L., Sande M. A. Infections with Cryptococcus neoformans in the acquired immunodeficiency syndrome. N Engl J Med. 1989 Sep 21;321(12):794–799. doi: 10.1056/NEJM198909213211205. [DOI] [PubMed] [Google Scholar]
  5. Dolan C. T. Specificity of the latex-cryptococcal antigen test. Am J Clin Pathol. 1972 Oct;58(4):358–364. doi: 10.1093/ajcp/58.5.358. [DOI] [PubMed] [Google Scholar]
  6. Gade W., Hinnefeld S. W., Babcock L. S., Gilligan P., Kelly W., Wait K., Greer D., Pinilla M., Kaplan R. L. Comparison of the PREMIER cryptococcal antigen enzyme immunoassay and the latex agglutination assay for detection of cryptococcal antigens. J Clin Microbiol. 1991 Aug;29(8):1616–1619. doi: 10.1128/jcm.29.8.1616-1619.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goodman J. S., Kaufman L., Koenig M. G. Diagnosis of cryptococcal meningitis. Value of immunologic detection of cryptococcal antigen. N Engl J Med. 1971 Aug 19;285(8):434–436. doi: 10.1056/NEJM197108192850804. [DOI] [PubMed] [Google Scholar]
  8. Gray L. D., Roberts G. D. Experience with the use of pronase to eliminate interference factors in the latex agglutination test for cryptococcal antigen. J Clin Microbiol. 1988 Nov;26(11):2450–2451. doi: 10.1128/jcm.26.11.2450-2451.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heelan J. S., Corpus L., Kessimian N. False-positive reactions in the latex agglutination test for Cryptococcus neoformans antigen. J Clin Microbiol. 1991 Jun;29(6):1260–1261. doi: 10.1128/jcm.29.6.1260-1261.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaufman L., Blumer S. Latex-cryptococcal antigen test. Am J Clin Pathol. 1973 Aug;60(2):285–286. doi: 10.1093/ajcp/60.2.285. [DOI] [PubMed] [Google Scholar]
  11. Kaufman L., Blumer S. Value and interpretation of serological tests for the diagnosis of cryptococcosis. Appl Microbiol. 1968 Dec;16(12):1907–1912. doi: 10.1128/am.16.12.1907-1912.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stockman L., Roberts G. D. Corrected version specificity of the latex test for cryptococcal antigen: a rapid, simple method for eliminating interference factors. J Clin Microbiol. 1983 May;17(5):945–947. doi: 10.1128/jcm.17.5.945-947.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wu T. C., Koo S. Y. Comparison of three commercial cryptococcal latex kits for detection of cryptococcal antigen. J Clin Microbiol. 1983 Nov;18(5):1127–1130. doi: 10.1128/jcm.18.5.1127-1130.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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