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. 1993 Jan;31(1):152–154. doi: 10.1128/jcm.31.1.152-154.1993

Detection of Francisella tularensis in blood by polymerase chain reaction.

G W Long 1, J J Oprandy 1, R B Narayanan 1, A H Fortier 1, K R Porter 1, C A Nacy 1
PMCID: PMC262641  PMID: 8417022

Abstract

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

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Selected References

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