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. 1993 Feb;31(2):289–291. doi: 10.1128/jcm.31.2.289-291.1993

Detection of hepatitis C virus RNA: comparison of one-stage polymerase chain reaction (PCR) with nested-set PCR.

D R Gretch 1, J J Wilson 1, R L Carithers Jr 1, C dela Rosa 1, J H Han 1, L Corey 1
PMCID: PMC262751  PMID: 8381810

Abstract

We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens were used. One-stage PCR may be less prone to contamination than nested-set PCR.

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Selected References

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